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iCE™ platforms give you reproducible, quantitative analysis of identity, purity, and heterogeneity profiles for your therapeutic proteins. And they do it fast—we're talking results in 15 minutes or less per sample! You'll also get high resolution, full-on automation, and none of the hassles that come with other IEF techniques. Maurice platforms also automate size separations for you, so you'll get robust and quantitative analysis for your monoclonal antibodies too. Get past your cIEF and CE-SDS characterization road-blocks with iCE!

iCE Systems for every application

Maurice iCE Systems
Features
cIEF Specification
CE-SDS Specification
Minimum Sample Volume
50 µL
50 µL
Sample Delivery
Vacuum
Electrokinetic
Typical Separation Time
6–10 min (molecule-dependent)
Reduced IgG: 25 min, Non-reduced IgG: 35 min
Detection Capability
UV Absorbance at 280 nm Fluorescence: Ex 280 nm, Em 320–450 nm
UV Absorbance at 220 nm
ypical Voltage
Pre-focusing: 1500 V, focusing: 3000 V
Separation: 5750 V
Sample Injections per Cartridge
100 guaranteed, 200 maximum
100 guaranteed, 200 maximum
Maximum Sample Injections per Batch
100
48
pI/Size Range
2.85–10.45
10–240 kDa
pI/Sizing CV
1%
≤2%
CV for Peaks >10% Composition
≤5% (Intra-batch), ≤6% (Inter-batch)
N/A
Relative Migration Time CV
N/A
<1% for reduced IgG
pI/Sizing Resolution
0.05 pI units (for wide range 3–10 ampholytes)
≥1.5 for NGHC/HC IgG Standard
Dynamic Range
2 logs
2 logs
Linearity
>0.995
>0.995
Sensitivity (LOD)
0.7 µg/mL (Native fluorescence) 3.0 µg/mL (Absorbance) (Values based on a monoclonal antibody)
0.3 µg/mL (Value based on Internal Standard)
Sample Tray Options
96-well plates or 48 vials
96-well plates or 48 vials
Maurice S. iCE Systems
Features
CE-SDS Specification
Minimum Sample Volume
50 µL
Sample Delivery
Electrokinetic
Typical Separation Time
Reduced IgG: 25 min, Non-reduced IgG: 35 min
Detection Capability
UV Absorbance at 220 nm
Typical Voltage
Separation: 5750 V
Sample Injections per Cartridge
100 guaranteed, 200 maximum
Maximum Sample Injections per Batch
48
Size Range
10–240 kDa
Sizing CV
≤2%
Relative Migration Time CV
<1% for reduced IgG
Sizing Resolution
≥1.5 for NGHC/HC IgG Standard
Dynamic Range
2 logs
Linearity
>0.995
Sensitivity (LOD)
0.3 µg/mL
(Value based on Internal Standard)
(Value based on Internal Standard)
Sample Tray Options
96-well plates or 48 vials
Maurice C. iCE Systems
Features
cIEF Specification
Minimum Sample Volume
50 µL
Sample Delivery
Vacuum
Typical Separation Time
6–10 min (molecule-dependent)
Detection Capability
UV Absorbance at 280 nm
Fluorescence: Ex 280 nm, Em 320–450 nm
Fluorescence: Ex 280 nm, Em 320–450 nm
Typical Voltage
Pre-focusing: 1500 V, focusing: 3000 V
Sample Injections per Cartridge
100
Maximum Sample Injections per Batch
100 guaranteed, 200 maximum
pI Range
2.85–10.45
pI CV
1%
CV for Peaks >10% Composition
≤5% (Intra-batch), ≤6% (Inter-batch)
pI Resolution
0.05 pI units (for wide range 3–10 ampholytes)
Dynamic Range
2 logs
Linearity
>0.995
Sensitivity (LOD)
0.7 µg/mL (Native fluorescence)
3.0 µg/mL (Absorbance)
3.0 µg/mL (Absorbance)
(Values based on a monoclonal antibody)
(Values based on a monoclonal antibody)
Sample Tray Options
96-well plates or 48 vials
Maurice iCE3 Systems
Features
Description
Sample Volume/Run
12-17 µL
Sample Delivery
Alcott 720 Autosampler or PrinCE Next Microinjector
Typical Run Time
10-15 minutes
Detection
UV absorption at 280 nm
Focusing Voltage
600 V/cm
Dimensions
66 cm H x 28 cm W x 31 cm D
Weight
20 kg (45 lbs)
Power
120-230 V AC, 50/60 Hz

Key Features

  • Ideal for cIEF and CE-SDS: PI and charge heterogeneity analysis data in less than 10 minutes—in both absorbance and native fluorescence detection modes and with sensitivity of detection down to 0.7 µg/mL. Their size applications have the high res and wide molecular weight range you need and they're done in 35 minutes.
  • User friendl: Just pop in a ready-to-go cartridge, drop in your sample vials or a 96-well plate, and hit start
  • Time savings: Your cIEF and CE-SDS methods are done in a day. You can develop platform methods for multiple molecules too. No maintenance and clean-up needed between the two applications.
  • Highly accurate and reliable:  Reproducible results with tight CVs. Data is reliable across samples, users, instruments or labs.
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ProteinSimpleProteinSimple’s goal is simply to help researchers gain a better understanding of proteins and their role in disease. ProteinSimple has built a unique portfolio of protein analysis tools including systems for traditional protein imaging, systems to probe the structure and purity of protein based therapeutics and systems that provide fresh insight into protein signaling.
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