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ProteinSimple - Simple Western Systems - Wes™

Wes™ lets you separate and analyze proteins by size from 2-440 kDa. He also gets you down to pg-level sensitivity with just 3 µL of starting material. Got a lot of samples and no time? No problem. Wes runs up to 25 samples in 3 hours flat and gives you quantitative, size-based data including total protein. Don't wait to discover, get going today with Wes!

Technical Specifications

Description Total Protein Specification ImmunoAssay Specification
Sample Required 0.3-1.2 µg 0.6-1.2 µg
Volume Required 3 µL/well 3 µL/well
Size Range Molecular weight ladder ranges from 12-230 kDa and 66-440 kDa Molecular weight ladder ranges from 12-230 kDa and 66-440 kDa
Sizing CV <10% <10%
Intra-assay CV <15% <15%
Inter-assay CV <20% <20%
Resolution ±10% difference in molecular weight ±10% difference in molecular weight
Quantitation CV <20% <20%
Dynamic Range 2-3 logs 3-4 logs
Sensitivity ng Low pg
Capillary 5 cm, 100 µm, 400 nL 5 cm, 100 µm, 400 nL

Key Features

  • Screen and optimize up to 75 samples in just 3 hours
  • HDR detection profile and analysis Wes produces up to 6 logs of dynamic range for reduced analysis time
  • With Wes you only need 3 µL of sample per capillary, so you can maximize the data you get per experiment
  • Reproducible with achievable intra-assay CV<15%

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ProteinSimpleProteinSimple’s goal is simply to help researchers gain a better understanding of proteins and their role in disease. ProteinSimple has built a unique portfolio of protein analysis tools including systems for traditional protein imaging, systems to probe the structure and purity of protein based therapeutics and systems that provide fresh insight into protein signaling.