Vaccination is the best approach to protect individuals and progress has been made in their development. In this webinar, we will discuss how the Microfluizer technology and dynamic light scattering can provide a range of useful characterization information for vaccine applications.
The Microfluidizer processors can be used in various applications such as to deliver nucleic acid-based vaccines (i.e. RNA). Two of the more promising methods are to use both viral and non-viral vectors. The Adeno associated viral vectors are one of the more commonly seen viral vectors and these vectors are often harvested from mammalian or insect cells, which can be lysed using a Microfluidizer processor. Non-viral vectors such as lipid-based nanoparticles (LNPs) have several benefits in nucleic based vaccines which includes both the hydrophilic and hydrophobic agents that can be encapsulated together with high efficiency. The LNPs can be coated with inert and biocompatible polymers, prolonging the liposomes’ circulating half-life in the body. Some properties of the LNPs can be adapted to aid vaccine efficacy such as the type of phospholipid used, surface modifiers to increase circulation time in the body and to avoid particles being destroyed by the immune system, and also the processing method (i.e. thin film method using Microfluidizer technology). These properties can affect the final vaccine development such as the particle size, which is particularly important for LNPs, as smaller particles allow better circulation throughout the body.
The Malvern dynamic light scattering technology can aid in the analysis of the intensity fluctuations in a small amount of sample to obtain the size distribution of the sample and to check for aggregates and particle size stability. Particles and molecules from 0.3 nanometers to 10 microns can be analysed by the Malvern Zetasizer. It is an analytical tool that delivers unique and supporting data for the quantification, identification, and characterization of bioparticles.
Who should attend?
Anyone keen to know more about the Microfluidizer and Dynamic light scattering technologies.
Date: Wednesday, 8 July 2020
Time: 2:00p.m. - 3:00p.m. (SGT)
Type: Online Webinar
Bernadette Lim, DKSH Singapore Pte Ltd
📞Tel. (+65) 9632 7282
✉ Email: email@example.com
Microfluidics International Corporation, the manufacturer of Microfluidizer® high shear fluid processors, is a leader in the design and production of laboratory and commercial processing equipment used in the production of micro- and nano-scale materials for pharmaceutical, biotech, chemical and diverse industries. Microfluidizer® technology enables some of the world’s top companies to create superior products, develop intellectual property, improve process efficiency and capitalize on new revenue streams. They provide the standard for nanoemulsion and nanoparticle applications.
Malvern Panalytical technologies enable the measurement of parameters including particle size, shape and concentration, chemical identity, zeta potential, protein charge, molecular weight, mass and conformation, biomolecular interactions and stability, rheological properties, elemental concentrations and crystallographic structures. Highly reliable and robust characterization of these properties is fundamental to predicting how a product will behave during use, to optimizing its performance and achieving manufacturing excellence.
The M110P is a premium "Plug & Play" electric benchtop laboratory homogenizer. Using the Microfluidics fixed-geometry Interaction ChamberTM technology and a ceramic (Zirconia) plunger, the M110P is capable of successfully processing a wide variety of fluids, including oil-in-water emulsions, solids-in-liquid suspensions and performing cell disruption of yeasts and plant cells — much of which is performed with as few as one or two passes.
Zetasizer Ultra is the world’s most capable combined DLS and ELS system, incorporating Non-Invasive Back Scatter (NIBS®) and, uniquely, Multi-Angle Dynamic Light Scattering (MADLS) technology for the measurement of particle and molecular size. NIBS provides the versatility and sensitivity to measure over a wide concentration range, while MADLS permits a higher resolution view into your sample’s size distribution for those critical measurements.
An extension to MADLS affords the ability to directly analyze particle concentration. The measurement of particle concentration is calibration-free, suitable for a wide range of materials, requires no or little dilution, and is quick to use – all of which make it ideal as a screening technique. This is a unique capability of the Zetasizer Ultra which can even be applied to samples such as viruses and VLPs, which were previously very challenging to measure.