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This gene codes for one of six actin proteins, playing a crucial role in cell movement, structure, and signaling. It's a key component of the contractile apparatus and is linked to Baraitser-Winter syndrome 1, causing intellectual disability and unique facial features. Many similar gene copies exist in the human genome.

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Available models

Reactivity

Human, Mouse, Rat

Recommended Dilution

WB 1:2000 - 1:20000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200

Antibody Dilute Procedure

Put 5ul AC004 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.

Storage Buffer

Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.

Application Key

Western blotting Immunohistochemistry Immunofluorescence

Positive Samples

293T, HeLa, A-549, NIH3T3, Mouse brain, Rat testis, C6

Cellular Location

Cytoplasm, cytoskeleton

HC(Mouse kidney, Rat kidney, Homo sapiens, Mus musculus)

WB(Mus musculus, Homo sapiens, Crassostrea gigas, Rattus norvegicus, Citrus sinensis, Sus scrofa, Ctenopharyngodon idellus, Cynoglossus semilaevis, chicken eggs, Spodoptera frugiperda, Pinellia ternata, Arabidopsis thaliana, Actinopterygii, Danio rerio , insect , Plutella xylostella, Bombyx mori Linnaeus, Lineage, Canis lupus familiaris, Homo sapiens、Mus musculus, Lampetra japonica, Staphylococcus aureus, mouse, Ictalurus punctatus, Rattus norvegicus,Mus musculus, Homo sapiens,Danio rerio)

IF(Homo sapiens)

IF(Homo sapiens, Mus musculus)

IP(Homo sapiens)

WB(Homo sapiens)

IP(Homo sapiens)

IHC(Mus musculus)

IP(Homo sapiens)

Co-IP(Homo sapiens)

Reactivity

Human, Mouse, Rat, Chicken, Zebrafish, Pig

Recommended Dilution

WB 1:10000 - 1:100000

Storage Buffer

Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.

Application Key

Western blotting

Positive Samples

293T, HeLa, Mouse brain, Mouse testis, Mouse lung, Rat brain, Rat testis, Rat lung

Cellular Location

Cytoplasm, cytoskeleton

WB(Sanghuangporus vaninii, Homo sapiens, Mus musculus, Rattus norvegicus, Bos taurus, Gallus gallus, Homo sapiens,Rattus norvegicus, Bacillus cereus)

IF(Mus musculus)

IP(Mus musculus)

real-time pcr(Homo sapiens)

ABclonalBased in Massachusetts, ABclonal aims to improve the accuracy and precision of life science research for scientists around the world by providing high-quality, personalized biology research reagents and services. Our large catalog of off-the-shelf products can be customized to ensure you can have the antibodies, peptides, and proteins best suited to you.
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