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Product outline:

  • A µ-Slide used to investigate angiogenesis in tube formation assays. Also ideal for 3D cell culture and immunofluorescence staining.
  • Complete solution for tube formation experiments, requiring only a few steps from sample preparation to image analysis
  • Brilliant visualization without meniscus formation, and with all cells in one focal plane
  • Can be used with a broad range of gels (e.g., Matrigel®, collagen, and agarose)
  • Cost-effective experiments, requiring only 10 µl of gel per well

Applications

  • Tube formation assays
  • 3D cell culture
  • Immunofluorescence staining
  • Sprouting assays
  • Live cell imaging of attached cells

Technical features

µ-Slide Angiogenesis
  • Standard slide format
  • Closely fitting lid to reduce evaporation
  • 4 mm well within a 5 mm well
  • Geometry enables pipetting of a homogeneous 0.8 mm thick gel layer*
  • Provides homogeneous cell growth
  • Compatible with staining and fixation
  • Excellent optical properties for microscopy
  • Compatible with multi-channel pipettes
  • Made of biocompatible plastic material: no glue, no leaking
  • Suitable for use with various types of gels* (e.g., Matrigel®, collagen, and agarose)
  • Principle for Wound Healing and Migration Assays

*The gel matrix is not included with this product.

Every Cell in Focus: The Principle of the µ-Slide Angiogenesis

The μ-Slide Angiogenesis has a specialized geometry for the easy, convenient, and reproducible conduction of tube formation assays. It is also ideal for sprouting assays, immunofluorescence staining, and general 3D cell culture.

After the gel has been pipetted into the inner well and given time to solidify, the cells can be seeded on top of it for tube formation analysis. Due to the “well-in-a-well” technology, the amount of gel needed is reduced to only 10 μl per well, which is 10% of the amount used in regular multiwell plates.

Further, no meniscus is formed. This ensures the formation of a uniformly thick gel matrix on which all cells are in one optical plane, creating reproducible cell culture conditions. The µ-Slide Angiogenesis can be used with all common hydrogel matrices, such as Matrigel®, collagen gels, agarose gels, and hyaluronic acid gels.

With its 15 wells, the µ-Slide Angiogenesis is designed for low throughput assays. For large scale applications, ibidi provides the μ-Plate Angiogenesis 96 Well.

Tube Formation Assays Using the µ-Slide Angiogenesis

Ibidiibidi is a leading supplier for functional cell-based assays and advanced products for cellular microscopy. ibidi is located in Gräfelfing, Germany, close to Munich, and the US headquarters, ibidi USA Inc., is located in Fitchburg, Wisconsin.
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