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Product outline:

  • Investigate chemotaxis of fast or slow migrating adherent cells and non-adherent cells in 2D or 3D gel matrices
  • Real-time chemotaxis measurement in real-time in a 2D or 3D environment
  • Stable chemotactic gradients for long-term experiments
  • Reproducible results with reliable and user-independent data

Applications

  • 2D and 3D chemotaxis assays with fast or slow migrating cells
  • Live cell imaging using inverted microscopy
  • Chemotaxis of neutrophils, lymphocytes, and monocytes
  • 3D chemotaxis of leukocytes or cancer cells in an ECM-like matrix
  • Invasion assays of tumour cells in Matrigel™
  • Chemotaxis measurements of adherent and non-adherent cells

Technical specifications

Outer dimensions (w x l) 25.5 x 75.5 mm²
Chemotaxis chambers on slide 3
Volume per chamber 120 µl
Observation area 2x1 mm²
Coating area per chamber Coating area per chamber
- When coating full chamber - When coating full chamber
- When coating observation area only
3.50 cm2 3.50 cm2
0.27 cm2 0.27 cm2
Distance between chambers 18.5 mm
Total height with plugs 12 mm
Volume chemoattractant 30 µl
Bottom ibidi Polymer Coverslip

Technical features

µ-Slide Chemotaxis
  • Chamber geometry optimized for cells in 2D and 3D matrices
  • Defined linear gradient with long-term stability
  • Homogeneous cell distribution at the experimental starting point
  • 3 chambers on one slide for parallel assays
  • Ideal for collagen gels, hydrogels, Matrigel™, or similar aqueous gels
  • Ready to use, i.e., no assembly required
  • Lettered and numbered chambers and reservoirs

Chemotaxis Assays Using the ibidi µ-Slide Chemotaxis

Ibidiibidi is a leading supplier for functional cell-based assays and advanced products for cellular microscopy. ibidi is located in Gräfelfing, Germany, close to Munich, and the US headquarters, ibidi USA Inc., is located in Fitchburg, Wisconsin.
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