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A µ-Slide with one channel and three wells for culturing cells on a 3D gel matrix with defined flow

Product outline:

  • Create a perfusable cell monolayer on a gel matrix for various flow and shear stress applications
  • Establish and image endothelial barriers under in vivo-like conditions—no artificial filters or membranes
  • Easy handling: samples are placed into the open wells; perfusion is applied after closing the wells

Applications

  • Culture of adherent cells on a 3D gel matrix under flow conditions
  • Transendothelial migration studies under flow conditions
  • Establishment and microscopic read-out of endothelial barrier assays (without any artificial membrane)
  • Simulation of blood vessels with endothelial cell culture on a soft substrate
  • Long-term or short-term cell culture perfusion experiments with defined shear stress and optimal nutrient supply into 3D structures
  • Apical-basal cell polarity assays
  • Cell barrier model assays with apical-basal gradients
  • Cell boundary assays without filters or porous membranes
  • Rolling and adhesion assays using leukocytes
  • Inflammation studies
  • Transport studies

Technical specifications

Outer dimensions (w x l) 25.5 x 75.5 mm²
Number of wells 3
Volume of wells 16 μl
Well dimensions 5.4 mm x 4.0 mm
Well height (without channel) 0.8 mm
Growth area per well 0.21 cm²
Coating area per well 0.34 cm²
Channel width 5.0 mm
Channel volume (without wells) 150 μl
Channel height (without well) 0.6 mm
Adapters Female Luer
Volume per reservoir 60 μl
Top cover ibidi Polymer Coverslip
Bottom ibidi Polymer Coverslip

Slide comparison

µ-Slide Spheroid Perfusion µ-Slide I Luer 3D µ-Slide I Luer
Application
Perfusion of samples Yes Yes Yes
Defined shear stress on cell monolayers No Yes, on gel matrix Yes, on coverslip
Gel matrices for 3D No Yes No
Cell type
Spheroids/organoids Yes, free floating in well Yes, inside gel matrix only No
Suspension cells Yes, free floating in well Yes, inside gel matrix only Yes, in flow suspension only
Adherent cells Yes, on coverslip Yes, inside or on gel matrix Yes, on coverslip
Ibidiibidi is a leading supplier for functional cell-based assays and advanced products for cellular microscopy. ibidi is located in Gräfelfing, Germany, close to Munich, and the US headquarters, ibidi USA Inc., is located in Fitchburg, Wisconsin.
[ibidi, µ-Slide I Luer 3D, Luer 3D]