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A perfusable channel µ-Slide with 3 x 7 wells for long-term spheroid cultivation and high-end microscopy

Product outline:

  • Ideal for the long-term cultivation and perfusion of 3D spheroids or organoids on the Bioinert surface
  • Flexible preparation for perfusion: spheroids can be transferred or created directly in the wells
  • Imaging chamber with excellent optical quality for high-resolution microscopy

Applications

  • Cultivation and high-resolution microscopy of three-dimensional cell aggregates (e.g., spheroids and organoids)
  • For use with the ibidi Pump System or any other pump device with Luer connectors
  • Perfusion of spheroids for fresh medium supply during long-term cultivation and access to spheroid growth kinetics
  • Spheroid generation directly in the wells
  • Spheroid retrieval for downstream processing (e.g., immunofluorescence, histology, and biochemical assays)
  • Perfusion of cell monolayers (adherent cells), suspension cells, or co-cultures
  • Organ-on-a-chip setups with up- and downstream metabolization
  • Live cell imaging and microscopy of 3D cell aggregates
  • Immunofluorescence staining and high-resolution fluorescence microscopy of living and fixed cells and cell aggregates

Technical specifications

Outer dimensions (w x l) 25.5 x 75.5 mm²
Number of channels 3
Number of wells 3 x 7
Volume per well 3.5 μl
Well height (bottom niche) 0.4 mm
Well height (total) 1.3 mm
Well diameter (bottom) 0.8 mm
Channel volume (total) 45 µl
Channel height 0.2 mm
Channel width 1.0 mm
Growth area per well 0.5 mm²
Coating area using 3.5 µl 9.7 mm²
Adapters Female Luer
Volume per reservoir 60 µl

Slide comparison

µ-Slide Spheroid Perfusion µ-Slide I Luer 3D µ-Slide I Luer
Application
Perfusion of samples Yes Yes Yes
Defined shear stress on cell monolayers No Yes, on gel matrix Yes, on coverslip
Gel matrices for 3D No Yes No
Cell type
Spheroids/organoids Yes, free floating in well Yes, inside gel matrix only No
Suspension cells Yes, free floating in well Yes, inside gel matrix only Yes, in flow suspension only
Adherent cells Yes, on coverslip Yes, inside or on gel matrix Yes, on coverslip
Ibidiibidi is a leading supplier for functional cell-based assays and advanced products for cellular microscopy. ibidi is located in Gräfelfing, Germany, close to Munich, and the US headquarters, ibidi USA Inc., is located in Fitchburg, Wisconsin.
[Ibidi, µ-Slide, Spheroid Perfusion, µ-Slide Spheroid Perfusion]