In HPLC, components of a mixture are carried through the stationary phase by the flow of a mobile phase and separation is based on differences in migration rates among the sample components. Therefore, the nature of your analytes defines not only the method but also the HPLC system.
Main characteristics of the analytes
The elutropic series defines the solvent strength for the most often used chromatography modes normal phase and reversed phase.
Key Features
Application area
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HPLC
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---|---|
Pump system
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Gradient
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Gradient
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Quaternary LPG
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Injection
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Autosampler
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Detector
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Diode array detection DAD
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Software
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ClarityChrom, Mobile Control
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Pressure
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862 bar
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Column thermostat
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Yes
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Eluent delivery
The Pump P 6.1L with LPG configuration has a new LPG valve for precise mixing efficiency of up to four eluents. The integrated degasser prevents air bubbles in the system. A mixing chamber is mounted on each Pump P 6.1L. This can be adapted to the respective application as required.
Sample Injection
The Autosampler 6.1L can inject 0.1 – 5000 µl to have a large flexibility. Using standard vials or two microtiter plates will give you a maximum sample capacity of up to 768 samples or 108 vials.
Column thermostating
The Column thermostat CT 2.1 is suitable for column thermostatization above and below room temperature. The instrument operates with a microprocessor controlled Peltier element for exact temperature settings.
Chromatography data system
KNAUER ClarityChrom® is a powerful, yet easy-to-use chromatography software for instrument control, data acquisition and data processing. It is an economical solution compared to other more complex chromatography software while still offering FDA 21 CFR Part 11 compliance.
Detection
The detector DAD 2.1L features a novel light path and flow cell architecture with improved handling and excellent performance. This Diode array detector can be used in the wavelength range of 190-700 nm.