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Microcalorimeters for characterization of biomolecular stability and interactions

MicroCal microcalorimeters are powerful, analytical tools used to study biological systems. These include interactions between molecules as well as changes in conformation such as protein unfolding. The technique is very convenient requiring only minimal assay development and NO labelling. The range includes both Differential Scanning and Isothermal Titration calorimetry.

MicroCal microcalorimeters are used in a wide range of applications from basic research to the discovery and development of small molecule drugs, biotherapeutics and vaccines. Our customers include pharmaceutical, biotech, academic and governmental research institutions worldwide.

MicroCal ITC range - Determine the binding parameters of biomolecules in a single analysis

MicroCal ITC isothermal titration calorimeters are used to study a wide range of biomolecular interactions. The applications range from drug design to fundamental research such as the understanding and regulation of signal transduction pathways. These systems deliver direct, label free and in-solution measurements of binding affinity and thermodynamic parameters in a single experiment. They have high sensitivity, low sample consumption and options for automation to minimize ‘hands on’ time.

MicroCal PEAQ-ITC Automated - Fully automated, hands free ITC for increased productivity

The MicroCal PEAQ-ITC Automated is a configurable, low volume, highly sensitive isothermal titration calorimeter which offers the convenience of unattended operation. It delivers direct, label-free in solution measurement of all binding parameters in a single experiment. Applications include characterizing molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules.

MicroCal PEAQ-ITC - The Gold Standard for measuring affinity and stoichiometry

The MicroCal PEAQ-ITC is a highly sensitive, low volume isothermal titration calorimeter for the label-free in solution study of biomolecular interactions. It delivers direct measurement of all binding parameters in a single experiment and can analyze weak to high affinity binders, using as little as 10µg sample. Semi-automated maintenance minimizes operator intervention and the system is upgradable to the fully automated MicroCal PEAQ-ITC Automated, making it ideal for laboratories where speed, sensitivity and the ability to accommodate higher workloads in the future are paramount.

MicroCal DSC range - Powerful analytical tools for characterizing the stability of proteins and other biomolecules

MicroCal DSC microcalorimeters are powerful analytical tools for characterizing the stability of proteins and other biomolecules, requiring minimal assay development, no labelling or immobilization. The automation and high sensitivity afforded by MicroCal VP-Capillary DSC systems permits screening of large numbers of samples at low concentrations minimizing the consumption of valuable samples with little hands-on effort.

MicroCal PEAQ-DSC Automated - Gold standard protein stability analysis for the regulated environment

The high-throughput, high-sensitivity MicroCal PEAQ-DSC Automated system features software that streamlines workflows through simplified experimental setup and flexible instrument scheduling. Automated data analysis supports the generation of high integrity thermal stability data and delivers compliance with regulatory requirements, whilst allowing easy integration into existing data handling and transfer systems.

MicroCal PEAQ-DSC - Gold standard protein stability analysis for research applications

The high-sensitivity MicroCal PEAQ-DSC system features automated data analysis to support the generation of high integrity thermal stability data and deliver compliance with regulatory requirements, whilst allowing easy integration into existing data handling and transfer systems. Unattended operation of the instrument after sample loading frees operator time.  This complete system requires no additional accessories, reagents or consumables.

 

Available Models

Malvern MircoCal PEAQ-ITC Automated
Technical Specifications
Measurement
Enthalpy,Affinity,Entropy,Stoichiometry
Temperature range
2°C to 80°C
Temperature stability
± 0.00012°C
Sample capacity
384 (four 96 well plates)
Sample volume
370 µL
Cell volume
200 µL
Injection syringe volume
40 µL
Injection volume precision
<1% @ 2µL
Throughput
Up to 42 per 24 hr
Cell material
Hastelloy
Noise
0.15 ncal/s
Response time
8 s

High productivity measurement of multiple binding parameters

The MicroCal PEAQ-ITC Automated is a configurable, low volume, highly sensitive isothermal titration calorimeter which offers the convenience of unattended operation. It delivers direct, label-free in solution measurement of all binding parameters in a single experiment. Applications include characterizing molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules. It can also be used to measure enzyme kinetics

 

Malvern MircoCal PEAQ-ITC
Technical Specifications
Measurement
Enthalpy,Affinity,Entropy,Stoichiometry
Temperature range
2°C to 80°C
Temperature stability
± 0.00012°C
Sample capacity
384 (four 96 well plates)
Sample volume
280 µL
Cell volume
200 µL
Injection syringe volume
40 µL
Injection volume precision
<1% @ 2µL
Throughput
8-12 per 8 hr
Cell material
Hastelloy
Noise
0.15 ncal/s
Response time
8 s

High sensitivity measurement of all binding parameters

The MicroCal PEAQ-ITC Automated is a configurable, low volume, highly sensitive isothermal titration calorimeter which offers the convenience of unattended operation. It delivers direct, label-free in solution measurement of all binding parameters in a single experiment. Applications include characterizing molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules. It can also be used to measure enzyme kinetics

Technical Specifications
Sample capacity
288 (6 × 96-well plates)
Sample volume
325 µL
Typical sample concentration
0.01 mg/mL – 10 mg/mL 1
Sample throughput
≤50 samples/day
Sample storage temperature range
4°C - 40°C
Operating temperature (°C)
+10°C to +28°C
Storage temperature
-20°C to +50°C
Humidity
10% to 70%, non-condensing (10% to 90% for storage)
Ingress Protection (IP) rating
IP21
Power
100-240 V A/C, 50/60 Hz, 70 W (cell), 400 W (max, autosampler), PC as supplied
Certification
CE (EN61010-1), EMC (EN61326-2-1, EN61326-1, FCC, ICES, VCCI), ISO9001:2008
Dimensions (W x D x H)
101 cm × 68 cm × 70 cm
Weight
Approx. 25 kg

Gold standard protein stability analysis for the regulated environment

The MicroCal PEAQ-DSC Automated system delivers high throughput, highly sensitive protein stability analysis with low sample consumption, in an automated, integrated platform for increased productivity.  All cell filling and cleaning functions are fully automated for walk-away operation.

 

Key Features

  • Gold standard stability-indicating technique requiring minimal assay development 
  • Direct and label-free measurement of biomolecular native-state stability in solution 
  • Standard 96-well plate format for high capacity and easy loading, with thermostatically-controlled storage of up to 6 plates
  • Fully integrated autosampler enables unattended analysis of up to 50 samples per day
  • Automated cell filling and cell cleaning functions permit unattended operation 
  • Measurement of very tight binding constants, up to 1020M-1
  • Powerful MicroCal PEAQ-DSC software minimizes typical data analysis time
  • PEAQ-Compliance: The system is available with Malvern Access Controller (MAC) to limit access to user-defined SOPs and data analysis features. Data can be displayed using the Report Generator with user details and the ability to sign using electronic signatures to facilitate the integration of the workflow into your company’s quality system and aid compliance with 21 CFR Part 11 and Annex 11 regulations. This is provided as an optional accessory
  • PEAQ-Performance: Automatically detects and validates system readiness for optimal performance
  • PEAQ-Smart (including PEAQ-Finder): For SOP-based operation and data analysis.  Provides new algorithms to detect even subtle peaks and shoulders, facilitating the automatic, non-subjective identification of multiple transitions, such as those seen in multi-domain proteins
  • PEAQ-Compare: For the quantitative comparison of DSC traces for comparability studies, ideal for batch-to-batch and biosimilarity studies
  • Network-ready: email updates sent during the analysis to keep you informed of assay progress
Technical Specifications
Sample volume
250 µL (manual filling)
Typical sample concentration
0.01 mg/mL – 10 mg/mL 1
Sample throughput
≤6 samples analyzed/8h
Operating temperature (°C)
+10°C to +28°C
Storage temperature
-20°C to +50°C
Humidity
10% to 70%, non-condensing (10% to 90% for storage)
Ingress Protection (IP) rating
IP21
Power
100-240 V A/C, 50/60 Hz, 70 W (cell), PC as supplied
Certification
CE (EN61010-1), EMC (EN61326-2-1, EN61326-1, FCC, ICES, VCCI), ISO9001:2008
Dimensions (W x D x H)
20 cm × 44 cm × 19 cm
Weight
8.2 kg
1 Sample dependent

Gold standard protein stability analysis for research applications

The MicroCal PEAQ-DSC system provides highly sensitive, easy-to-use microcalorimetry that helps reduce the time and cost associated with stability testing and comparability analysis.  The MicroCal PEAQ-DSC is a manual instrument with a cleaning device, which can be upgraded to an automated version upon request.

 

Key Features

  • Gold standard stability-indicating technique requiring minimal assay development
  • Direct and label-free measurement of biomolecular native-state stability in solution 
  • Measurement of very tight binding constants, up to 1020M-1
  • Manual system with pipette and cleaning device
  • Powerful MicroCal PEAQ-DSC software minimizes typical data analysis time
  • PEAQ-Compliance: The system is available with Malvern Access Controller (MAC) to limit access to user-defined SOPs and data analysis features. Data can be displayed using the Report Generator with user details and the ability to sign using electronic signatures to facilitate the integration of the workflow into your company’s quality system and aid compliance with 21 CFR Part 11 and Annex 11 regulations. This is provided as an optional accessory
  • PEAQ-Performance: Automatically detects and validates system readiness for optimal performance
  • PEAQ-Smart (including PEAQ-Finder): For SOP-based operation and data analysis.  Provides new algorithms to detect even subtle peaks and shoulders, facilitating the automatic, non-subjective identification of multiple transitions, such as those seen in multi-domain proteins
  • PEAQ-Compare: For the quantitative comparison of DSC traces for comparability studies, ideal for batch-to-batch and biosimilarity studies
  • Network-ready: email updates sent during the analysis to keep you informed of assay progress
  • Measurements of binding affinity and thermodynamic parameters in a single experiment

  • High sensitivity with options for automation to minimize ‘hands on’ time

  • Low sample consumption

Learn about Microcal Range with DKSH expert

What Is Differential Scanning Calorimetry (DSC)?

Malvern Instruments MicroCal DSC team introduce what Differential Scanning Calorimetry (DSC) is and why it is the gold standard in characterizing the stability of a protein or other biomolecules.

The Malvern MicroCal team details how Differential Scanning Calorimetry (DSC) works and why you should be using it for testing protein stability.

  • Biopharmaceutical and Biotechnology
  • Chemical
  • Medical
  • Pharmaceutical
Malvern PanalyticalMalvern Panalytical was formed by the merger of Malvern Instruments Limited and PANalytical B.V. on 1st January 2017, has headquarters in both Almelo (the Netherlands), and in Malvern (UK), and employs over 2,000 people worldwide. The combined entity is a strong player and innovator in the materials characterization market and will leverage the strengths of the individual companies in their end markets, ranging from building materials to pharmaceuticals and from metals and mining to nanomaterials.
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