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Waters offers a wide array of column choices and formats, including but not limited to HPLC, UPLC, UHPLC, GPC, SFC, and SEC.  Select the ideal column for your application, and simplify your methods development, validation, and transfer throughout your organization.

Our fully scalable particle technologies ensure that our LC columns perform with a broad range of chromatographic instrumentation. Depending on the goals of a separation, the instrument platform used, or the sample type, you can choose the most suitable column that is matched to your system’s configuration without adversely affecting the chromatographic result.

Our quality mission is to ensure that the Waters’ Columns you use today are the most reproducible and reliable LC columns available. As a primary manufacturer of silica and hybrid particles, scientists can be assured of consistent column performance, batch-to-batch reproducibility, and product availability over the life of the analytical method.

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MaxPeak Premier Columns

Stop Looking and Start Seeing

MaxPeak Premier Columns are designed to be the most universal column platform for chromatographers who need to reduce variability risks and save time, while increasing recovery and sensitivity. Non-specific adsorption (NSA) of metal sensitive compounds is an unpredictable challenge, leading to long column passivation times, chromatography with large RSDs, and broad peaks that can be a challenge to detect. MaxPeak Premier Columns feature MaxPeak High Performance Surfaces (HPS) which effectively reduce NSA losses due to metal interactions and provide:

  • Reduced column passivation
  • Increased reproducibility
  • Enhanced sensitivity
  • Improved peak shapes

Atlantis Premier Columns

Atlantis Premier Columns

Next-generation hybrid particle and surface technology for increased polar analyte retention

Retaining polar compounds is one of the most challenging chromatographic problems. With the hybrid particle technology in Atlantis Premier BEH C18 AX and the BEH Z-HILIC Columns, those problems are a thing of the past. These reversed-phase, anion-exchanged, mixed-mode, and zwitterionic columns are the next evolution of columns in the Atlantis family, all designed to achieve superior polar compound retention.

Atlantis Premier BEH C18 AX and the BEH Z-HILIC Columns combine a new and innovative chromatographic particle technology with proprietary MaxPeak High-Performance Surfaces (HPS) Technology to boost retention of polar acidic compounds by 50% while eliminating unwanted analyte-surface interactions.

XBridge Premier Columns

XBridge Premier Columns

Particle Stability with the Protection of MaxPeak HPS

XBridge Premier 2.5 µm Columns are the evolution of Waters XBridge 2.5 µm XP (eXtended Performance) columns. They combine the industry leading column stability of Ethylene Bridged Hybrid (BEH) particles with MaxPeak High Performance Surface (HPS) Technology. XBridge Premier Columns are available in a standard column format or a VanGuard FIT (Fully Integrated Technology) column format. The VanGuard FIT Column is an all-in-one guard and column design that’s easy-to-use, and extends column lifetimes when analyzing samples in challenging matrices.

XBridge Premier 2.5 µm Columns are part of the MaxPeak Premier Class of columns. Designed to be the most universal column platform, XBridge Premier Columns prevents undesired analyte/surface interactions by utilizing MaxPeak HPS Technology. MaxPeak HPS is designed to increase analyte recovery, sensitivity, and reproducibility by minimizing analyte/surface interactions that can lead to sample losses. In the MaxPeak Premier Class of columns, non-specific adsorption is dramatically reduced for analytes that may bind to metal surfaces, without sacrificing performance for those that do not.

With a reduction in these undesired interactions, you can expect:

  • Reduced column conditioning and passivation, leading to higher productivity
  • Improved sensitivity and peak shapes, leading to better quality data
  • Increased reproducibility from analysis to analysis, leading to more confidence in analytical results

XSelect Premier Columns

XSelect Premier Columns

Maximize selectivity with problem-solving technologies for a wide range of analytes

Get the best injection to injection reproducibility in your chromatographic separations with Waters XSelect Premier 2.5 µm Columns, which utilize MaxPeak High Performance Surfaces (HPS) Technology. Ideal for the wide range of analytes encountered in method development, XSelect Premier Columns combine modern particle technologies and ligands with the protection of MaxPeak HPS Technology. 

Designed to maximize selectivity, XSelect Premier Columns are built on one of two innovative base particle technologies, Charged Surface Hybrid (CSH) and High Strength Silica (HSS). The integration of MaxPeak HPS allows you to achieve balanced, controlled interactions between your analytes, mobile phase, and the analytical column for the highest performance chromatographic separations.

UPLC Columns

More than 200 combinations of column configurations and chemistries, UPLC Columns combine faster separations with higher resolution and sensitivities by harnessing the full potential of small particles. All Waters UPLC Columns are designed, tested, and guaranteed for routine use in applications up to 18,000 psi (1241 bar).

ACQUITY UPLC Columns

ACQUITY UPLC Columns

Increase speed, sensitivity, and resolution, without compromising data integrity

Increasing sample throughput, achieving unmatched resolution, and improving analyte sensitivity are priorities for your separation goals. With Waters ACQUITY UPLC Columns, you can meet all your method requirements and substantially reduce your analysis time and cost-per-sample. 

Waters ACQUITY UPLC Columns’ sub-2-μm particle chemistries enable chromatographers to harness the separation efficiency improvements at increased flow rates of ultra-performance liquid chromatography (UPLC) technology, thereby improving analyte resolution and sensitivity as well as business productivity and profitability.  

CORTECS Columns

CORTECS Columns

Solid-core columns that live up to their potential.

Your lab needs to develop methods for challenging separations even faster. Increase resolution for even your toughest samples with the enhanced column efficiency of CORTECS solid-core columns, which provide the flexibility and power you need to rapidly separate a wide array of compounds. 

CORTECS Columns are available in reversed-phase and HILIC options across seven column chemistries with different particle characteristics and innovative charged surface modifications for 20% faster separations* and 25% more resolution at low operating back pressures.

VanGuard Column Protection

VanGuard Column Protection

Protect and prolong analytical column lifetime

Even under the best conditions, analytical LC columns deteriorate over time from chemical or particulate contamination. With VanGuard Column Protection products, you can now achieve maximum lifetime and performance from your columns.

VanGuard column products are optimized to protect and prolong analytical column lifetimes without compromising chromatographic performance. Available in a wide selection of particle sizes and stationary phases across HPLC, UHPLC, and UPLC, VanGuard Column Protection products are ideally suited for the physical and chemical protection of all Waters analytical columns.

Atlantis Premier Columns

Atlantis Premier Columns

Next-generation hybrid particle and surface technology for increased polar analyte retention

Retaining polar compounds is one of the most challenging chromatographic problems. With the hybrid particle technology in Atlantis Premier BEH C18 AX and the BEH Z-HILIC Columns, those problems are a thing of the past. These reversed-phase, anion-exchanged, mixed-mode, and zwitterionic columns are the next evolution of columns in the Atlantis family, all designed to achieve superior polar compound retention.

Atlantis Premier BEH C18 AX and the BEH Z-HILIC Columns combine a new and innovative chromatographic particle technology with proprietary MaxPeak High-Performance Surfaces (HPS) Technology to boost retention of polar acidic compounds by 50% while eliminating unwanted analyte-surface interactions.

nanoACQUITY UPLC Columns

nanoACQUITY UPLC Columns

nanoACQUITY Trap and Separation Columns

nanoACQUITY Columns and trap columns are the previous generation of columns for nano- and microflow LC-MS applications. They are being replaced by the new generation of ACQUITY UPLC M-Class columns:

  • Higher pressure rating (15,000 psi/1,034 bar) enable higher flow rates and thus shorter run times and higher sample turnover.
  • M-Class Columns are compatible with nanoACQUITY UPLC systems.

UHPLC Columns

Our focused selection of UHPLC columns highlight the performance advantages modern UHPLC instrumentation. Limiting your column choices limits your ability to fully develop the potential of your analytical laboratory. Waters’ CORTECS and eXtended Performace (XP) Ultra-High Performance LC columns and sorbents provide capability that will surpass your current and future analytical challenges.

CORTECS Columns

CORTECS Columns

Solid-core columns that live up to their potential.

Your lab needs to develop methods for challenging separations even faster. Increase resolution for even your toughest samples with the enhanced column efficiency of CORTECS solid-core columns, which provide the flexibility and power you need to rapidly separate a wide array of compounds. 

CORTECS Columns are available in reversed-phase and HILIC options across seven column chemistries with different particle characteristics and innovative charged surface modifications for 20% faster separations* and 25% more resolution at low operating back pressures.

XBridge BEH XP Columns

XBridge BEH XP Columns

Universal Column Family

The common base particle for all XBridge BEH HPLC, eXtended Performance (XP) and ACQUITY UPLC Columns provide seamless scalability throughout the available particle sizes (1.7, 2.5, 3.5, 5, and 10 μm). Improve existing HPLC methods 4-fold by selecting a column configuration that provides the correct balance between resolution and sample throughput.

XSelect Columns

XSelect Columns

Achieve unmatched reproducibility with a wide range of selectivity

Quickly and easily develop robust methods compatible with all modern chromatographic detection modes and transfer them from lab to lab with the XSelect Column family.

Designed to help you achieve reproducible and highly selective chromatographic separations, the XSelect Column family features two base particles technologies, Charged Surface Hybrid (CSH) and High Strength Silica (HSS). With XSelect Columns, method development scientists like you can achieve a wide range of selectivities across all application areas, without compromising performance.

VanGuard Column Protection

VanGuard Column Protection

Protect and prolong analytical column lifetime

Even under the best conditions, analytical LC columns deteriorate over time from chemical or particulate contamination. With VanGuard Column Protection products, you can now achieve maximum lifetime and performance from your columns.

VanGuard column products are optimized to protect and prolong analytical column lifetimes without compromising chromatographic performance. Available in a wide selection of particle sizes and stationary phases across HPLC, UHPLC, and UPLC, VanGuard Column Protection products are ideally suited for the physical and chemical protection of all Waters analytical columns.

HPLC Columns

State-of-the-art Reversed-Phase and HILIC HPLC Columns

Waters offers a wide range of analytical and preparative chromatography columns to meet the needs of virtually every application. The Waters Column Advisor recommends the most appropriate column depending on the on the application requirements you need to consider and the column characteristics you are looking for. Separations developed on our analytical columns can be directly scaled up to preparative columns. We supply a wide range of phase chemistries in numerous column dimensions for preparative work to meet your scale-up needs. All chromatography columns meet stringent specifications to ensure excellent performance and column-to-column reproducibility. We also offer a wide range of accessoriesto support these columns.

CORTECS Columns

CORTECS Columns

Solid-core columns that live up to their potential.

Your lab needs to develop methods for challenging separations even faster. Increase resolution for even your toughest samples with the enhanced column efficiency of CORTECS solid-core columns, which provide the flexibility and power you need to rapidly separate a wide array of compounds. 

CORTECS Columns are available in reversed-phase and HILIC options across seven column chemistries with different particle characteristics and innovative charged surface modifications for 20% faster separations* and 25% more resolution at low operating back pressures.

XBridge BEH XP Columns

XBridge BEH XP Columns

Universal Column Family

The common base particle for all XBridge BEH HPLC, eXtended Performance (XP) and ACQUITY UPLC Columns provide seamless scalability throughout the available particle sizes (1.7, 2.5, 3.5, 5, and 10 μm). Improve existing HPLC methods 4-fold by selecting a column configuration that provides the correct balance between resolution and sample throughput.

XSelect Columns

XSelect Columns

Achieve unmatched reproducibility with a wide range of selectivity

Quickly and easily develop robust methods compatible with all modern chromatographic detection modes and transfer them from lab to lab with the XSelect Column family.

Designed to help you achieve reproducible and highly selective chromatographic separations, the XSelect Column family features two base particles technologies, Charged Surface Hybrid (CSH) and High Strength Silica (HSS). With XSelect Columns, method development scientists like you can achieve a wide range of selectivities across all application areas, without compromising performance.

VanGuard Column Protection

VanGuard Column Protection

Protect and prolong analytical column lifetime

Even under the best conditions, analytical LC columns deteriorate over time from chemical or particulate contamination. With VanGuard Column Protection products, you can now achieve maximum lifetime and performance from your columns.

VanGuard column products are optimized to protect and prolong analytical column lifetimes without compromising chromatographic performance. Available in a wide selection of particle sizes and stationary phases across HPLC, UHPLC, and UPLC, VanGuard Column Protection products are ideally suited for the physical and chemical protection of all Waters analytical columns.

Symmetry HPLC Columns

Symmetry HPLC Columns

Silica-based columns that exceed HPLC column performance expectations

Reproducible HPLC columns are essential when developing analytical methods for pharmaceutical and biopharmaceutical products. Achieving the same chromatographic results over the lifetime of a new drug product is critical, and no other silica-based LC column brand can match the column-to-column and batch-to-batch reproducibility of the Symmetry family.

Available in column and guard formats, Symmetry Columns are manufactured using high purity silica and tightly controlled manufacturing processes to ensure that you receive a column that exceeds the standards for HPLC column performance.

Atlantis Columns

Atlantis Columns

Reversed-phase and HILIC HPLC Columns for superior polar compound retention

Polar analytes are difficult to retain using traditional reversed-phase (RP) chromatography as they often elute in the void volume of the column. You can solve this challenge today with Atlantis Columns, which retain and separate polar analytes under reversed phase and HILIC conditions and provide quick, accurate, and reproducible quantification with increased retention and sensitivity.

Available in T3, dC18, and the unbonded HILIC chemistries, the Waters Atlantis Column family provides exceptional performance, versatility, retention, and chromatographic stability for polar compounds and balanced retention for broad analyte mixtures. 

SunFire Columns

SunFire Columns

Superior mass loading at low pH

High efficiency at a low pH can be challenging. Waters SunFire HPLC Columns, available in analytical and preparative dimensions, help you do just that with state-of-the-art bonded silica designed for high mass loading, excellent low pH stability, superior peak shapes, and high efficiency.

SunFire Analytical Columns are available in 2.5 µm to 10 µm particle sizes across two reversed-phase column chemistries, C18 and C8, sure to meet your silica-based HPLC column requirements. For preparative applications, in addition to the C18 and C8 chemistries, SunFire silica is offered in 5 and 10 µm particle sizes for preparative normal-phase purification.

XTerra Columns

XTerra Columns

Optimal selectivity at any pH for more robust separations

Historically, limitations of the stationary phase material have imposed restrictions on speed, resolution, pH, temperature, and loading capacity for scientists doing HPLC separations. XTerra HPLC Columns retain the advantages of silica without restrictions on speed, resolution, pH/temperature instability and loading.

XTerra Columns, which use the first generation of Hybrid Particle Technology (HPT), broke these boundaries and enabled scientists like you to realize the full potential of analytical and preparative separations.

Resolve your toughest separation challenges with XTerra Column chemistries, which include C18, C8, and Phenyl chemistries providing a wide range of selectivity.

Nova-Pak Columns

Nova-Pak Columns

Scale-up from analytical to prep, simplified

Reducing solvent consumption while maintaining resolution for complex mixtures is important, but that’s a challenge with larger particle sizes. With the smaller particle sizes and shorter column lengths in Nova-Pak Columns, those problems are a thing of the past. The bonded phases of Nova-Pak Columns are available in 4 µm and 6 µm particle sizes, offering high resolution and faster, more efficient chromatography over columns packed with larger particle sizes.

Nova-Pak Analytical Columns with 4 µm particle size packing offer a range of length options with 75, 100, 150, and 300 mm length steel columns. The semi-preparative Prep Nova-Pak HR Columns are packed with 6 µm particle size packings for unparalleled separation possibilities. The high-efficiency packing of Prep Nova-Pak HR Columns provides faster separations with less solvent for the added advantage of more concentrated fractions—reducing your preparative chromatography cost. To ensure batch-to-batch reproducibility, all Nova-Pak C18, C8, Cyano and Phenyl Columns are packed according to stringent quality control (QC) procedures in Waters cGMP manufacturing facility.

Waters Spherisorb HPLC Columns

Waters Spherisorb HPLC Columns 

HPLC Columns for diverse separation selectivities

When you’re developing your methods, you don’t want to leave anything to chance – especially whether your columns will work. That’s not a problem with Waters Spherisorb Columns, one of the most widely referenced HPLC columns in scientific literature. With more than 2,000 analytical abstracts published using these columns, Spherisorb Columns provide a tremendous range of validated methods and applications to assist in your method development process.

And with a wide range of particle sizes (3, 5, and 10 µm) and high-quality bonded phases for different and unique separation selectivities, there’s sure to be a Waters Spherisorb Column to meet your analytical and preparative column chromatography needs. 

Resolve Columns

Resolve Columns

High resolution spherical packings

Non-endcapped Resolve packings are significantly different from Waters other packing materials in that they typically provide higher retention of polar compounds and complement those of Nova-Pak and µBondapak chemistries. Resolve C18 and silica columns are available in 5 µm and 10 µm spherical packings for applications requiring higher resolution than what is achievable using irregularly shaped chromatographic particles. The non-endcapped silica surface offers a balance of retention for separation of polar compounds.

µBondapak and Bondapak Columns

µBondapak and Bondapak Columns

The original µBondapak C18 packing material

Scale from analytical to prep and separate acidic, basic, and neutral compounds with µBondaPak and BondaPak Columns, the only columns with the original µBondaPak C18 packing material.

While many companies may claim “μBondapak-like” selectivity, none have passed Waters stringent quality control (QC) batch tests. By passing these tests, μBondapak and BondaPak packing materials have ensured reproducibility for Waters customers year after year since 1973 — becoming one of the most widely referenced HPLC column brands.

µPorasil/Porasil Columns

µPorasil/Porasil Columns

Normal-phase separations for a wide array of sample types

μPorasil and Porasil particles were one of the first commercially available, fully porous packing materials used for LC separations. In contrast to the reversed-phase separation ability of μBondapak C18, the non-bonded, silica-based material in μPorasil Columns was produced to provide normal-phase separations for a wide array of sample types.

With a range of different particle size offerings, you have the option of scalability for preparative application with µPorasil column as a QC tool.

Waters PAH Columns

Waters PAH Columns

Improved Analysis of PAH Compounds

Polyaromatic hydrocarbons (PAHs) are among the most frequently monitored environmental contaminants. Standard and official methods for the analysis of PAHs are found in compendia for air, drinking water, wastewater, solid waste, and food analysis. Many of these methods specify HPLC, usually with UV and fluorescence detection, as the recommended analytical procedure. Waters PAH columns are optimized for the HPLC analysis of PAHs. These columns achieve baseline resolution and excellent peak symmetry for 16 different target analytes in less than 25 minutes, while employing a simple water/acetonitrile binary gradient method. The resolving power of the PAH columns provides superior peak shape identification and quantitation for PAHs. They come in seven different dimensions (including a capillary format) and two particle sizes.

Protein-Pak Columns

Protein-Pak Columns

Analysis and lab scale isolation of proteins or polypeptides

Protein-Pak LC Columns contain either well-performing silica or methacrylate-based, ion-exchange, size-exclusion, and hydrophobic interaction, and affinity column chemistries for the separation, lab-scale purification, and characterization of proteins and other biomolecules.

The Protein-Pak HPLC and UPLC Column family includes:

  • Hi Res HIC columns
  • Hi Res ion-exchange (IEX) columns
  • Ion-exchange glass columns
  • Affinity epoxy-activated columns

ACQUITY APC Columns

Advanced Polymer Chromatography

ACQUITY APC™ Columns deliver advanced sorbent technology for separating complex synthetic polymer and macromolecular species. They deliver superior performance and reliability for high resolution polymer characterization compared to conventional GPC columns. ACQUITY APC Columns improve advanced polymer chromatography by:

  • Improving Bed Stability. ACQUITY APC Columns eliminate the shrinking and swelling that often occurs with polymeric stationary phases upon solvent switching.
  • Maximizing Efficiency. The optimized pore volumes and particle sizes provide unmatched separation speed, resolution and chromatographic efficiency.
  • Providing Industry Leading Reproducibility. Each step of our column manufacturing process is tightly controlled to deliver exceptional product reliability.

ACQUITY APC Columns

Bioseparations Columns & Consumables

Innovating Solutions to Help Solve Your Toughest Challenges

Our scientists, engineers, and application chemists continue to develop innovative columns and sample preparation consumables that support HPLC, UPLC, UHPLC, LC-MS analyses or lab-scale purification of oligonucleotides, amino acids, peptides, proteins and glycans. Our promise is to provide you with:

  • Solutions you can count on: Reproducible and robust columns and systems, QC tested the way you use it
  • Results you can trust: Wide range of technical expertise/application notes, Data integrity with our informatics solutions
  • Flexibility that you require: Scalability/Transferability (UPLC/UHPLC/HPLC), Easy-to-use automation

AccQ•Tag Ultra, AccQ•Tag and Pico•Tag Columns

AccQ•Tag Ultra, AccQ•Tag and Pico•Tag Columns

UPLC, UHPLC, and HPLC-Based Methods for Accurate Amino Acid Composition Analysis

Waters AccQ•Tag and Pico•Tag technologies enable your laboratory to obtain accurate amino acid composition from protein/peptide hydrolysates, physiologic fluids, feeds, foods, pharmaceutical preparations, and myriad other samples using traditional HPLC or UHPLC  instrumentation. The Waters AccQTag Ultra Column is designed for use on Waters ACQUITY UPLC platforms to deliver superior amino acid component resolution and in less time compared to use of more traditional HPLC or UHPLC methods.

AccQ•Tag and Pico•Tag products include:

  • Pre-column derivatization reagents
  • Dedicated reversed-phase columns QC tested in application
  • HPLC / UHPLC or UPLC based eluents and standards
  • Documented methods

Amino Acid Analysis

Amino Acid Analysis

Amino Acid Analysis

Determination and control of critical nutrients in cell culture is important to maximize protein yield and provide a consistent manufacturing process. The Waters UPLC®Amino Acid Analysis (AAA) Solution is a turnkey solution that provides accurate, high throughput amino acid analysis.

The UPLC Amino Acid Analysis Solution meets the specific demands of analyzing amino acids for protein characterization, cell culture monitoring, and nutritional analysis of foods and feeds. This end-to-end system is designed to deliver the accuracy and reliability you need to be successful.

Amino Acid Analysis Standards & Kits

Amino Acid Analysis Standards & Kits

AccQ•Tag Ultra, AccQ•Tag Ultra - Automation, and AccQ•Tag Sample Preparation for Accurate Amino Acid Composition Analysis

The amino acid sequence is the primary structure of a protein and it is unique to a particular peptide or protein. Determining amino acid composition in protein/peptide hydrolysates, foods, feeds, and pharmaceutical preparations is important. Amino acids are also key ingredients in the cell culture medium used to prepare recombinant proteins. It is often desirable to monitor on a regular basis the consumption of individual amino acids reaction in the bioreactor so the conditions in the bioreactor can be optimized.

Accurately separating, identifying, and quantitating amino acids is challenging. Waters provides a number of solutions that use pre-column derivatization so that amino acids can be easily analyzed. Kits are available in both manual and automated configurations.

BioResolve RP mAb Polyphenyl Consumables

BioResolve RP mAb Polyphenyl Consumables

Reversed-Phase Solutions for Intact and Ides Digested mAbs

An innovative solution for the analytical characterization of intact protein analysis of mAbs and ADCs. It includes a column chemistry that can be used on UPLC, UHPLC, and HPLC platforms; a relevant application-specific standard; and more information and support in the box. The benefits include:

  • Improved peak capacity for better component resolution
  • Minimal injection-to-injection carryover for increased confidence of analyses
  • Ability to operate at lower temperatures for less on-column sample degradation
  • Excellent performance regardless of LC system used
  • Ensured column-to-column performance by being QC tested with a NIST-based, mAb subunit standard

BioResolve SCX mAb Consumables

BioResolve SCX mAb Consumables

Cation-Exchange Solutions for mAb Charge Variant Analysis

An inclusive solution (columns, standards, and mobile-phase concentrates) for characterizing, monitoring, and quality control testing charge variants of mAb-based therapeutics from discovery and development through manufacturing. Scientists can easily obtain desired data using defined methods on any LC platform without sacrificing performance. The benefits include:   

  • Ability to reliably separate, with accurate charge variant quantitation, a wide range of mAbs using optical or LC-MS compatible mobile phases
  • Excellent batch-to-batch and column-to-column performance consistency
  • LC system (HPLC, UHPLC, UPLC) agnostic, doesn't compromise required separation performance
  • Waters, NIST-based, mAb Charge Variant Standard is available to help confirm LC system and column performance
  • QR-code enabled to help obtain relevant information anytime and anywhere

BioResolve SEC mAb Consumables

BioResolve SEC mAb Consumables

SEC Solutions for mAb Aggregate, Monomer, and Fragment/Clip Analyses

A solution purposely designed for organizations involved in the research and/or development of monoclonal antibody (mAb) drugs that require accurate quantitation of mAb aggregates (HMWS), monomers, and fragments/clips (LMWS); providing reliable, high-quality, SEC analytical data which addresses internal and regulatory concerns regarding these biotherapeutics. The BioResolve SEC mAb Columns and Guards provide:

  • Out-of-the-box performance
  • Tested with a relevant mAb size variant standard to help ensure consistent column-to-column and batch-to-batch performance, regardless of LC platform used
  • QR-code enabled to help obtain relevant information anytime and anywhere

HILIC for Large Biomolecules

HILIC for Large Biomolecules

An alternative, high resolution separation technology

Hydrophillic Interaction Chromatography (HILIC) has been widely used to separate small polar compounds, yet its application to large biomolecules, other than released glycans, has been surprisingly limited. With Waters widepore ACQUITY UPLC Glycoprotein BEH Amide 300 Å, 1.7 μm Column and new ideas on separation methods, it is now possible to use HILIC to glean previously unattainable information from intact proteins (with or without glycosylation), protein fragments, and complex, released glycans.

HILIC for Released Glycans

HILIC for Released Glycans

Robust LC or LC-MS Analyses of Biotherapeutic Glycoproteins

The analysis of complex N- and 0-linked glycans composed of frequently similar and repeating sugar moieties requires a highly discriminating chromatographic method. Hydrophilic liquid interaction chromatography (HILIC), using UPLC, HPLC, or UHPLC columns containing Waters amide bonded, Ethylene Bridged Hybrid (BEH) Technology particles separates compounds based on differing hydrophilicity.

Mixed Mode for Released Glycans

Mixed Mode for Released Glycans

Orthogonal Selectivity to HILIC Amide Columns

The separation of neutral-to-highly acidic released N-glycans by charge is valuable which quickly groups N-glycan species with different terminal residues and provides an understanding of the amount of sialyation and phosphorylation that is important for efficacy and safety of protein-based drugs. The Mixed-Mode C18 AX Chemistry Solution offers:

  • A reversed-phase/anion-exchange separation for supreme resolution
  • MaxPeak High Performance Surfaces (HPS) Technology that reduces analyte surface interactions, thereby improving recovery, sensitivity, and reproducibility
  • MS-compatibility
  • Suitability for multiple labels
  • An easy-to-use MS-grade mobile-phase concentrate
  • RapiFluor-MS Sialylated Glycan Performance Test Standard for benchmarking

Sialic Acid and Monosaccharide Analyses

Sialic Acid and Monosaccharide Analyses

Orthogonal Techniques for Quantitation

Evidence for reliable and consistent glycosylation of glycoprotein therapeutics is typically obtained through LC or LC-MS analysis of released N-linked glycans. However, drug discovery and development organizations, as well as regulatory agencies, frequently find value using orthogonal techniques to determine and/or confirm quantitative levels of sialic acids and monosaccharides contained in a therapeutic protein due to potential effect on biological activity and stability of the drug.

Waters offers UPLC, UHPLC, and HPLC-based C18 columns and defined application conditions that can assist scientists with the analysis and quantitation of these important glycan derived components.

Glycan Standard & Kits

Glycan Standard & Kits

Specially formulated and high-quality

Waters Glycan Standards and Kits are for released N-glycan labeling.  We offer standards in a wide variety of labels, 2-AB, 2-AA and RapiFluor-MS.  In addition, we offer complete kits for both 2-AB and RapiFluor-MS labeling in a variety of  sample sizes for low throughput to high throughput.

GlycoWorks RapiFluor-MS N-Glycan Kit

GlycoWorks RapiFluor-MS N-Glycan Kit

Reimagine Released N-Glycan Analysis

Waters has developed the GlycoWorks RapiFluor-MS N-Glycan Kit. This technology enables unprecedented fluorescent and mass spectrometric performance for glycan detection while also improving the throughput of N-glycan sample preparation.

  • 30-minute, 3-step streamlined workflow
  • Increased fluorescence quantification and supreme mass spectral response
  • One label that provides valuable information from characterization to routine monitoring

Automation Connected Devices and Accessories

Automation Connected Devices and Accessories

Scientific research demands the highest levels of reproducibility and traceability. Discover the ecosystem of connected devices that Andrew Alliance is building with partner organizations to support laboratory workflows.

  • Connected to OneLab by WiFi/ethernet for remote control and tracking
  • Can be used manually in combination with Pipette+ or directly with Andrew+ (like a Domino)
  • Easy to install with virtual support

Automation Workflow Kits and Scripts

Automation Workflow Kits and Scripts

Realize the full potential of your automation workstations with Waters sample preparation kits. Compatible with a variety of industry-standard platforms, seamlessly automate your Waters workflow to gain laboratory efficiency without encountering the issues inherent in scripting complex manual methods. 

  • Transition to routine, high-throughput analysis with Waters easily customizable, application-specific base scripts for Andrew Alliance, Hamilton, and Tecan platforms
  • Automation-ready application kits designed for various automation platforms
  • Verified platform scripts and detailed user guides to ensure seamless deployment and robust analytical method performance 

Otto SPEcialist Positive Pressure Manifold

Otto SPEcialist Positive Pressure Manifold

Improve SPE Reproducibility, Method Transfer, and Processing Efficiency

The Otto SPEcialist Positive Pressure Manifold is designed to further improve method robustness and sample reproducibility for solid-phase extraction (SPE). The semi-automated system, controlled by an interfaced tablet, and its easy-to-use software allows the same method to be used across multiple days, users, labs, and assays, removing the variability of manual extraction systems. The Otto SPEcialist is compatible to use with µElution and macro elution plates; and 1 cc, 3 cc, and 6 cc cartridges.

Enzymate Pepsin Column

Enzymate Pepsin Column

Faster, more efficient online protein digestion

Fast, efficient, and reproducible online pepsin digestion is critical in experiments that require more peptide coverage. With the Waters Enzymate BEH Pepsin Column, you can easily incorporate online protein digestion into existing HDX- MS workflows.

The Enzymate BEH Pepsin Column efficiently digests intact proteins into peptides in an online HDX system, typically in about 30 seconds. The peptic peptides are then retained on a trapping column and refocused onto a sub-2-μm ACQUITY UPLC Column for elution into high-resolution mass spectrometry.

MassPREP On-Line Desalting Cartridges

MassPREP™ on-line desalting cartridges are an effective tool for desalting acidic, basic, and large globular proteins.

The MassPREP desalting strategy provides an easy, fast (less than 3 minutes), and reproducible approach for the effective desalting of proteins stored in physiological salt buffers prior to high resolution ESI-MS structural analysis.

  • Effectively desalts proteins yielding improved ESI-MS results
  • Fast on-line method for high-throughput applications
  • Excellent protein recoveries and no detectable carryover
  • More than 100 injections from a single cartridge

MassPREP Micro Desalting Cartridges

MassPREP micro desalting cartiridges contains a polymer-based, reversed-phase packing material for desalting protein samples prior to mass spectrometry. The phenyl-phase material in these on-line devices successfully retains proteins, allowing the salts to be washed to waste prior to elution of the desalted pro­tein into the mass spectrometer. With an optimized LC/MS method, you may have cycle times as low as 4 minutes.

Nano- and Microflow LC-MS Columns

Nano- and Microflow LC-MS Columns

Separation and Trap Columns

Waters nano- and microflow LC-MS columns are specifically designed for low-dispersion nano LC systems.

The selected stationary phases fully leverage the separation power of sub-2-µm particle technology. This delivers the efficiency and selectivity required for complex separations such as discovery of low abundance species, peptide mapping, protein identification, and biomarker discovery.

  • Leverage the efficiency of sub-2-µm particle technology at high flow rates
  • Stationary phases with a variety of characteristics suitable for biomolecule separations
  • Easy interface with LC-MS systems

nanoACQUITY UPLC Columns

nanoACQUITY UPLC Columns

nanoACQUITY Trap and Separation Columns

nanoACQUITY Columns and trap columns are the previous generation of columns for nano- and microflow LC-MS applications. They are being replaced by the new generation of ACQUITY UPLC M-Class columns:

  • Higher pressure rating (15,000 psi/1,034 bar) enable higher flow rates and thus shorter run times and higher sample turnover.
  • M-Class Columns are compatible with nanoACQUITY UPLC systems.

Peptide Standards and Kits

Peptide Standards and Kits

Saving Valuable Time

Waters peptide standards and kits ensure productivity through reduction of instrument downtime.  These products also assist in the development, validation, and troubleshooting peptide applications.

  • Consistent Chromatography:  Recommended to be used on a regular basis for monitoring column and system performance
  • Quality:  Manufactured with strict quality control for batch-batch reproducibility
  • Ease of Use: Packaged in Waters vials for direct solubilization

DNA/RNA Oligonucleotide Separations

DNA/RNA Oligonucleotide Separations

Addressing Challenges in LC and LC-MS Based Analyses

Reversed-phase and ion-exchange chromatography for the analysis of synthetic oligonucleotides and related DNA/RNA species is well documented in their ability to purify and analyze these heterogeneous entities (e.g., full-length oligonucleotide product from undesired shorter and longer length sequences). With increased use of MS detection, Waters introduced the XTerra C18 family of "hybrid particles" that are MS compatible with increased high pH tolerance based on various application needs. Waters second-generation of XBridge C18 hybrid columns were introduced to expand performance characteristics.

  • Scalable offering in 1.7 μm UPLC particles,  2.5 μm HPLC/UHPLC particles, and 5 µm lab-scale preparative particles in various column dimensions.
  • Increased confidence in results: MaxPeak Premier Columns with MaxPeak HPS are available for those who have more challenging samples that are prone to metal affinity; providing improved sensitivity, recovery, reproducibility, and less time-consuming column conditioning. ACQUITY Premier and XBridge Premier Oligonucleotide Columns are available in 1.7 µm and 2.5 µm particle sizes.
  • Exceptional sample resolution and superior column life.
  • Confidence in results: All columns are QC tested with relevant application standards to help ensure consistent batch-to-batch and column-to-column performance.

Oligonucleotides Standards & Kits

Oligonucleotides Standards & Kits

Quality Control Reference Materials

Oligonucletotide separation columns deliver exceptional sample resolution and superior column life.  Now this chemistry has a powerful counterpart, application specific standards developed with the same methods used to run the column chemistry.  Designed in-house by our scientific experts, the standards in this portfolio are developed specifically with the column and system technologies.    

These standards are typically the same standards our quality control department uses to test the columns.  They are now offered directly to our customers to save valuable sample preparation and development time.

Peptide Reversed-Phase Columns

Peptide Reversed-Phase Columns

Reversed-Phase Chromatography

Reversed-phase chromatography using ion-pairing reagents such as TFA and formic acid can deliver highly resolved separations of complex peptide mixtures, (e.g., tryptic protein digests or lengthy synthetic peptide sequences) whose sequences may differ by a single amino acid. In general, the hydrophobicity of the peptide determines the elution order, with the least hydrophobic peptides eluting first.  

Factors such as particle composition (silica vs hybrids), pore size (130 Å vs 300 Å), ligand density, as well as separation conditions (e.g., gradient duration, separation temperature, flow rate) all play an important role in obtaining a separation that meets application requirements.

As with other bioseparations, when using smaller particle sizes, UPLC/UHPLC column offerings provide superior component resolution and in less time compared to HPLC-based columns for this demanding application.

Peptide Ion-Exchange Columns

Peptide Ion-Exchange Columns

Ion-Exchange Chromatography

Ion-exchange chromatography separates peptides based on charge differences and is used when a different / orthogonal separation mechanism is desired vs the more traditional use of  reversed-phase chromatography.

Separations are most commonly performed using a gradient of increasing salt concentration with the smaller, less charged peptide species eluting prior to the longer peptide fragments.   Unlike reversed-phase peptide separtions, eluent pH can significantly affect the separation so method development is required in order obtain satisfactory chromatography.

As with other bioseparations, use smaller particle size, UPLC/UHPLC offerings provide superior component resolution and in less time compared to our HPLC-based columns.

Peptide SEC Columns

Peptide SEC Columns

Size-Exclusion Chromatography

Size exclusion, also known as gel filtration, chromatography separates proteins based on their sizes (hydrodynamic radii) in solution and not by absolute molecular weight with larger sized species eluting before smaller peptides.  The primary driving mechanism for this isocratic based technique is based on the pore size and volume of the SEC particles used in the packed column.   SEC columns containing comparatively larger pore size particles are selected as the molecular weight of the peptides of interest increase.

As with other bioseparations, use smaller particle size, UPLC/UHPLC offerings provide superior component resolution and in less time compared to our HPLC-based columns for this demanding application.

RapiGest SF Surfactant

RapiGest SF Surfactant

Enhance the quality of in-solution enzymatic protein digestions

Labs around the globe are trying to simplify digestion protocols and improve throughput of analysis. Waters patented RapiGest SF Surfactant was designed with your lab’s efficiency in mind, significantly decreasing proteolytic digestion time, and enabling same-day digestions with mass spectrometry (MS) compatibility.

RapiGest SF radically enhances the speed and percent recovery of protein enzymatic digestions. This patented anionic surfactant accelerates the in-solution production of peptides generated by proteases, such as Trypsin, Asp-N, Glu-C, Lys-C, and PNGase F. Many hydrophobic proteins are resistant to proteolysis because their cleavage sites are inaccessible to endoproteases. RapiGest SF facilitates the digestion of proteolytically resistant proteins and increases the solubility of hydrophobic proteins and peptides. RapiGest SF is an MS-compatible mild protein denaturant that helps solubilize and unfold proteins, making them more amenable to cleavage without denaturing or inhibiting common proteolytic enzymes like conventional denaturants. This enables use of lesser amounts of expensive endoproteases.

Protein Standards

Protein Standards

Specially Formulated

Waters Protein Standards and Protein Mixes are specially formulated and designed to aid in the evaluation and benchmarking of columns designed and used for protein separations. Waters line of Protein Standards provide:

  • Consistent Chromatography:  Recommended to be used on a regular basis for monitoring column and system performance
  • Quality:  Manufactured with strict quality control for batch-batch reproducibility
  • Ease of Use:  Packaged in Waters Vials for direct solubilization

Oasis Sample Extraction Products

Oasis Sample Extraction Products

Faster, cleaner, and simpler sample preparation starts here

Laboratories worldwide would like simple sample preparation for robust method development. Get method development options with Waters Oasis sample extraction products, which come in a broad range of sorbents and an array of formats. With Oasis products, you can meet a wide variety of sample preparation needs, from simple and fast sample matrix clean-up to more complex sample preparation challenges.

Oasis Sample Extraction Products deliver unmatched purity, consistency, and quality for sample analysis. Available in seven different solid-phase extraction (SPE) chemistries, Oasis sorbents provide the range of options you need for method development.

Protein Ion-Exchange Columns

Protein Ion-Exchange Columns

Ion-Exchange Chromatography

IEX separations are most commonly performed using gradients of increasing salt, changing pH, or simultaneous salt increases and pH changes with less charged protein species eluting prior to more highly charged molecules. Based on protein type and separation pH, either an anion or cation exchanger is selected for the separation. In addition, gradient duration, buffer composition and pH, flow rate, as well as separation temperature, all play an important part in obtaining desired protein separations.

Protein SEC Columns

Protein SEC Columns

Size-Exclusion Chromatography

Size-exclusion chromatography (SEC), also known as gel filtration, separates proteins based on their sizes (hydrodynamic radii versus absolute molecular weight) in solution with larger sized species eluting before smaller proteins. The primary driving mechanism for this isocratic based technique is based on the pore size and volume of the SEC particles used in the packed column. As the molecular weight of the proteins of interest increases, SEC columns containing comparatively larger pore size particles are selected. Why choose BEH SEC column technology?

  • Less ionic interactions with stable Diol-coating for confidence in recovery and reproducibility
  • Unlike silica-based particles, BEH is pH stable >pH 10 for method flexibility
  • Outstanding column lifetime
  • Scalable chemistries for method transfer
  • Pore size options include 125 Å, 200 Å, and 450 Å depending on the protein of interest
  • Synthesized batches are QC tested with relevant samples to help ensure consistency

ProteinWorks

ProteinWorks

Reproducible and Standardized Protein Quantification with a Simplified Kit-Based Approach

Routinely achieve reproducible, accurate, and sensitive protein quantification with ProteinWorks, Waters new family of protein bioanalysis sample preparation kits. ProteinWorks eXpress Digest Kits simplify and accelerate protein digestion, streamlining, and standardizing the traditionally complex pre-analytic workflow for LC-MS protein quantification via the surrogate peptide approach.  The ProteinWorks uElution SPE Kit is designed for post-digestion clean-up, increasing assay sensitivity, and improving system robustness by removing excess digest reagents, phospholipids, and other plasma and serum components. With ProteinWorks, achieve:

  • Simplified sample preparation with pre-measured, lot-traceable reagents
  • Reproducible and accurate results for several monoclonal antibodies
  • Reduced time to results and cost of analysis

GPC & SEC Columns

For over 50 years, Waters has been the market leader in LC-based GPC and SEC analysis, providing the highest quality columns and applications support for both organic- and aqueous-based size separations. The names gel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel filtration chromatography (GFC) are commonly used terms to describe a liquid chromatographic technique that separates compounds based on their relative different sizes in solution. This isocratic-based separation is performed by injecting sample onto a carefully packed column(s) containing particles of defined pore size with the larger molecular weight species eluting before those compounds of smaller molecular weight (i.e., larger sized species elute prior to smaller sized compounds). Chromatographers with different backgrounds and application needs tend to refer to these same size-based separations by different terms. In general, the term GPC is used for those analyzing plastics or synthetic polymers (organic or water-soluble) using either organic or aqueous containing eluents. By comparison, SEC is more commonly used to describe size-based separations of biological compounds such as peptides and proteins.

Protein SEC Columns

Protein SEC Columns

Size-Exclusion Chromatography

Size-exclusion chromatography (SEC), also known as gel filtration, separates proteins based on their sizes (hydrodynamic radii versus absolute molecular weight) in solution with larger sized species eluting before smaller proteins. The primary driving mechanism for this isocratic based technique is based on the pore size and volume of the SEC particles used in the packed column. As the molecular weight of the proteins of interest increases, SEC columns containing comparatively larger pore size particles are selected. Why choose BEH SEC column technology?

  • Less ionic interactions with stable Diol-coating for confidence in recovery and reproducibility
  • Unlike silica-based particles, BEH is pH stable >pH 10 for method flexibility
  • Outstanding column lifetime
  • Scalable chemistries for method transfer
  • Pore size options include 125 Å, 200 Å, and 450 Å depending on the protein of interest
  • Synthesized batches are QC tested with relevant samples to help ensure consistency

GPC & SEC Columns

GPC & SEC Columns

Gel permeation chromatography (GPC) is a chromatographic technique that separates molecules based on their relative size in solution. As such, gel permeation and size exclusion chromatography (SEC) are synonymous terms along with gel-filtration chromatography (GFC) and gel chromatography. However, the terms "gel permeation chromatography” or “GPC” are often used by scientists who analyze polymers (plastics or other synthetic polymers) using either organic or aqueous eluents. In some cases, GFC may be used to refer to GPC analysis for aqueous soluble polymers (e.g., polysaccharides, cationic, anionic, and amphoteric polymers). Waters offers GPC/SEC columns for both organic and aqueous polymer separations, and SEC columns for monoclonal antibodies, proteins, peptides, and other biologics. 

Nano and Micro LC Columns

Columns designed for reliable nano, capillary, and microscale UPLC separations

Waters nano columns offer purposefully-designed hardware to meet the different demands of today's nanoflow LC and LC-MS separations. The UPLC Column delivers superior chromatographic resolution and fully leverages the separation power of small sub-2-µm particles. The selected stationary phases for nano-LC and trapping columns provide an advantage developing fast and efficient separations for complex peptide and protein separations and other sample limited analyses.

  • ACQUITY UPLC M-Class Columns (rated for 15,000 psi  [1,000 bar]) allow for faster separations and are compatible with the nanoACQUITY UPLC system.
  • The iKey Separation Device is an enabling technology behind the ionKey/MS System. It eliminates the need for traditional fittings and greatly simplifies the microflow-LC user experience.

Nano- and Microflow LC-MS Columns

Nano- and Microflow LC-MS Columns

Separation and Trap Columns

Waters nano- and microflow LC-MS columns are specifically designed for low-dispersion nano LC systems.

The selected stationary phases fully leverage the separation power of sub-2-µm particle technology. This delivers the efficiency and selectivity required for complex separations such as discovery of low abundance species, peptide mapping, protein identification, and biomarker discovery.

  • Leverage the efficiency of sub-2-µm particle technology at high flow rates
  • Stationary phases with a variety of characteristics suitable for biomolecule separations
  • Easy interface with LC-MS systems

ionKey/MS

ionKey/MS

Enhanced MS with the Turn of a Key

The ionKey/MS System redefines ultimate performance by integrating microflow UPLC directly into the source of the mass spectrometer.  This provides an increased level of sensitivity, chromatographic performance, and ease-of-use that cannot be achieved any other way.

  • The 150 µm I.D. iKey Separation Device achieves up to 40X increase in sensitivity compared to 2.1 mm UPLC columns
  • The 300 µm I.D. iKey HT Separation Device allows higher sample throughput with short cycle times
  • Simplified microflow LC-MS user experience
  • UPLC separations with sub-2-µm particle technology
  • Robust, reproducible, and reliable microflow LC-MS analyses

nanoACQUITY UPLC Columns

nanoACQUITY UPLC Columns

nanoACQUITY Trap and Separation Columns

nanoACQUITY Columns and trap columns are the previous generation of columns for nano- and microflow LC-MS applications. They are being replaced by the new generation of ACQUITY UPLC M-Class columns:

  • Higher pressure rating (15,000 psi/1,034 bar) enable higher flow rates and thus shorter run times and higher sample turnover.
  • M-Class Columns are compatible with nanoACQUITY UPLC systems.

Preparative (Purification) HPLC Columns

Preparative (Purification) HPLC Columns

Preparative (Purification) HPLC Columns

Bridging the Gap from Analytical to Prep

As a medicinal chemist working on a challenging drug discovery project, or as purification scientist attempting to maximize yield, your background in chromatographic separations has meant that up until recently you have mainly worked with other chemists to successfully isolate compounds. However, the decline in the number of drugs approved for release onto the market in recent years has put pressure on you and your team to refine the drug discovery process or better refine your purification process and use more effective methods to increase throughput.

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WatersWaters Corporation, the world's leading specialty measurement company, has pioneered chromatography, mass spectrometry and thermal analysis innovations serving the life, materials and food sciences for nearly 60 years. With approximately 7,000 employees worldwide, Waters operates directly in 31 countries, including 15 manufacturing facilities, and with products available in more than 100 countries. Waters Corporation's businesses include Waters, TA Instruments, VICAM, ERA, and Nonlinear Dynamics. Waters creates business advantages for laboratory-dependent organizations by delivering ultra performance liquid chromatography (UPLC), high-performance liquid chromatography (HPLC), chromatography columns and chemistry products, mass spectrometry systems, laboratory informatics solutions, and comprehensive service programs to enable significant advancement in healthcare delivery, environmental management, food safety, and water quality.
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