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The PN5300 automatic sample injector includes the latest state-of-the-art auto injector technology, such as a double needle with positive headspace pressure, extensive wash routines for minimal carry over and three injection modes including micro liter pick-up mode for zero sample loss, huge injection range and full biocompatibility. Offering space for two deep well, shallow well or vial rack adapter plates, the PN5300 is ideally suited to be integrated with a wide range of different FFF systems. The newly designed Peltier cooling/heating keeps samples stable in a closed environment for maximum reproducibility. The temperature range from 4°C up to 40°C prevents degradation, evaporation or precipitation of sample during storage and processing with any FFF system.