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Pacbio – Short-read Sequencing – Onso System

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Pacbio – Short-read Sequencing – Onso System

The Pacbio – Short-read Sequencing – Onso System is an innovative benchtop short-read DNA sequencing platform with an extraordinary level of accuracy using PacBio sequencing by binding (SBB) technology. Ultra-high Q40+ data quality allows you to break through limits of detection.

The Onso short-read sequencing system is a scalable and flexible benchtop platform that provides an extraordinary level of accuracy through its unique sequencing by binding (SBB™) technology. With inherently lower sequencing noise, the Onso system enables researchers to uncover rare variants previously missed and do so at lower sequencing depths.

The accuracy provided by the Onso system will help drive groundbreaking discoveries in areas such as liquid biopsy testing for minimal residual disease (MRD), antibiotic resistance monitoring in microbial communities, off-target analysis in gene editing experiments, and many other “needle-in-a-haystack” applications where the highest level of sensitivity is desired.

Sequencing Specifications

Onso reagents	Read length	Reads	Output (GB)	Run time	Quality score
200 cycle sequencing kit	2x100 bp 1x200 bp	400—500 M	80—100	32 hours	≥90% Q40
300 cycle sequencing kit	2x150 bp	400—500 M	120—150	48 hours	≥90% Q40

Technical Specifications

Temperature	18–26°C (64–79°F)
Humidity	20–80% relative humidity, non-condensing
Altitude	Below 2,000 m (6,562 ft)
Sequencer dimension (W x D x H)	37 in (94 cm) × 29 in (73.7 cm) × 25.75 in (65.4 cm)
Weight	133.8 kg (295 lb)
Cluster generator dimension (W x D x H)	32 in (81.3 cm) x 22 in (55,9 cm) x 26 in (66 cm)
Weight	43.5 kg (96 lb)
Power requirements	100–240 V at 50–60 Hz
Network connection	≥10 Gbps
ICC operating system	Windows 10 Enterprise
Output file format	FASTQ
Output file size	35–60 GB

Key Features

15× higher accuracy than other benchtop sequencers

SBB chemistry to deliver ultra-high quality, highly accurate, and exceptionally sensitive detection of cfDNAs.

Unprecedented sensitivity

Delivering the ability to identify biomarkers missed by other sequencing platforms.

Q40+ performance provides high quality results at lower read depth than SBS

Less sequencing per sample allows reduced cost and increased throughput.

Seamless workflow integration

Designed with key accessories and the flexibility to integrate with existing short-read tools:

Library conversion kit
PacBio native library prep kits (HMW and fragmented DNA preparation)
Blocking oligos for hybridization capture methods
Library amplification kit
Library quantification kit
Lambda sequencing control library (spike-in library)

A Groundbreaking New Sequencer

PacBio offers revolutionary sequencing technologies that combine the completeness of long reads with the accuracy of short reads to provide the most comprehensive view of genomes, transcriptomes, and epigenomes.