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The Pacbio – Short-read Sequencing – Onso System is an innovative benchtop short-read DNA sequencing platform with an extraordinary level of accuracy using PacBio sequencing by binding (SBB) technology. Ultra-high Q40+ data quality allows you to break through limits of detection.

The Onso short-read sequencing system is a scalable and flexible benchtop platform that provides an extraordinary level of accuracy through its unique sequencing by binding (SBB™) technology. With inherently lower sequencing noise, the Onso system enables researchers to uncover rare variants previously missed and do so at lower sequencing depths.

The accuracy provided by the Onso system will help drive groundbreaking discoveries in areas such as liquid biopsy testing for minimal residual disease (MRD), antibiotic resistance monitoring in microbial communities, off-target analysis in gene editing experiments, and many other “needle-in-a-haystack” applications where the highest level of sensitivity is desired.

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Sequencing Specifications

Onso reagents Read length Reads Output (GB) Run time Quality score
200 cycle sequencing kit 2x100 bp
1x200 bp
400—500 M 80—100 32 hours ≥90% Q40
300 cycle sequencing kit 2x150 bp 400—500 M 120—150 48 hours ≥90% Q40

Technical Specifications

Temperature 18–26°C (64–79°F)
Humidity 20–80% relative humidity, non-condensing
Altitude Below 2,000 m (6,562 ft)
Sequencer dimension (W x D x H) 37 in (94 cm) × 29 in (73.7 cm) × 25.75 in (65.4 cm)
Weight 133.8 kg (295 lb)
Cluster generator dimension (W x D x H) 32 in (81.3 cm) x 22 in (55,9 cm) x 26 in (66 cm)
Weight 43.5 kg (96 lb)
Power requirements 100–240 V at 50–60 Hz
Network connection ≥10 Gbps
ICC operating system Windows 10 Enterprise
Output file format FASTQ
Output file size 35–60 GB

Key Features

SBB chemistry to deliver ultra-high quality, highly accurate, and exceptionally sensitive detection of cfDNAs.

Delivering the ability to identify biomarkers missed by other sequencing platforms.

Less sequencing per sample allows reduced cost and increased throughput.

Designed with key accessories and the flexibility to integrate with existing short-read tools:

  • Library conversion kit
  • PacBio native library prep kits (HMW and fragmented DNA preparation)
  • Blocking oligos for hybridization capture methods
  • Library amplification kit
  • Library quantification kit
  • Lambda sequencing control library (spike-in library)

A Groundbreaking New Sequencer

PacBioPacBio offers revolutionary sequencing technologies that combine the completeness of long reads with the accuracy of short reads to provide the most comprehensive view of genomes, transcriptomes, and epigenomes.