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Confocal microscopes from Leica Microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented precision in three-dimensional imaging and exact examination of subcellular structures and dynamic processes.

Modular concept of our confocal microscopes

High-speed imaging supplies the data for a wide range of integrated analytical techniques. Our confocal microscopes are based on a modular concept that enables flexible upgrading and integration of innovative technology all the way to the nano range with STED 3X.

Available models

Super-resolution live cell imaging in multicolor SP8 LIGHTNING

The SP8 LIGHTNING confocal microscope allows you to make proper and detailed observations of fast biological processes. Your experimental work will have the benefit of super-resolution, high-speed imaging, and the capability to image multiple fluorescent markers simultaneously.

Thanks to the exclusive detection concept of LIGHTNING, you can trace the dynamics of multiple molecules, even those expressed at low levels, simultaneously over long recording times in living specimens.

The SP8 LIGHTNING confocal microscope offers you the following performance advantages

  • truly simultaneous multicolor imaging in super-resolution down to 120 nm
  • live specimen imaging thanks to fast acquisition rates
  • sample protection thanks to low phototoxicity

The SP8 LIGHTNING confocal microscope opens up unique experimental options to inspire your research!

Lifetime imaging in an instant SP8 FALCON

SP8 FALCON (FAst Lifetime CONtrast) is the future of functional imaging. Harness the power of fluorescence lifetime to investigate cellular physiology and explore dynamics in living cells. SP8 FALCON is the first truly integrated solution for Fluorescence Lifetime Imaging (FLIM) and delivers benchmark results at least 10x faster than conventional systems.

SP8 FALCON adds a new dimension of contrast to your imaging, opening the door to biosensing and tracking of interactions between proteins. FLIM information is now available for all modalities of the SP8 platform.

Now you can:

  • Follow fast molecular interactions via FLIM-FRET(Förster Resonance Energy Transfer)
  • Use biosensors to detect changes in metabolic state and microenvironment
  • Apply lifetime contrast to separate multiple fluorophores
  • Acquire fluorescence lifetime data with minimal training

Maximum information from each specimen LIGHTNING

Maximize the information you extract from your specimen and get in-depth answers to scientific questions with the LIGHTNING detection package for image information extraction.

LIGHTNING fully automatically detects the finest structures and details, which are otherwise simply not visible. The key technology of LIGHTNING is an adaptive process for extraction of hidden information in the image. Unlike traditional technologies, that use a global set of parameters for the full image, LIGHTNING calculates an appropriate set of parameters for each voxel to uncover every detail with the highest fidelity.

What’s more, the LIGHTNING detection concept extends the TCS SP8 confocal microscope by simultaneous multicolor super-resolution imaging at the full original speed.

With LIGHTNING, in one click you

  • reveal the most information from your images and resolve nanoscale structures down to 120 nm
  • obtain real-time results even for ambitious experimental conditions
  • maintain resolution and speed for multi-channel data acquisition

Deep In Vivo Explorer SP8 DIVE

The SP8 DIVE Deep In Vivo Explorer is the first spectrally tunable solution for multicolor, multiphoton (MP) imaging.

The SP8 DIVE provides spectral freedom. Equipped with 4Tune, a tunable, non-descanned detection system, the SP8 DIVE offers you unlimited flexibility and enables you to develop new multicolor deep in vivo experiments.

The SP8 DIVE provides maximum penetration depth. The new Vario Beam Expander can be tuned for improved penetration depth depending on the animal model. Capture twice as much of the fluorescence signal with the 4Tune detection. Achieve more contrast and depth for multicolor, deep in vivo imaging.

The SP8 DIVE gets the best out of your experiment!

STED Nanoscopes Leica TCS SP8 STED

Nanoscopy has revolutionized the study of subcellular architecture and dynamics and is on its way to becoming the new gold standard in fluorescence imaging (Nobel Prize for Chemistry 2014).

The fully integrated STED (STimulated Emission Depletion) systems Leica TCS SP8 STED 3X and Leica TCS SP8 STED ONE meet the requirements of daily research and provide fast, intuitive, and purely optical access to structural details far beyond the diffraction limit. 
Resolution becomes tunable in x, y and z.

LightSheet microscope Leica TCS SP8 DLS

Benefit from two dedicated imaging systems in one: With the Leica TCS SP8 DLS (Digital LightSheet) you get a full confocal with gentle single plane illumination in one microscope. Through the invention of the TwinFlect mirror, we turned our confocal platform Leica TCSSP8 into an easy-to-use, versatile light sheet microscope.

CARS Microscope - Label Free Imaging Leica TCS SP8 CARS

Staining is unnecessary when imaging specimens with the TCS SP8 CARS (Coherent anti-Stokes Raman Scattering) confocal microscope. The CARS technology exploits image contrast arising from characteristic vibrational states of different molecules within a specimen. As no labeling is required, the specimen remains almost unaffected from preparation and imaging.

Users benefit from its ability to image, at high speed and resolution, a wide range of live specimens, including cells, tissues, and even small organisms. To achieve this performance, the microscope offers visible, infrared (IR), and ultraviolet (UV) lasers, second harmonic generation (SHG), and CARS.

Due to its flexibility and convenient handling, the TCS SP8 CARS is a superb instrument for research institutes and multi-user facilities.

White Light Laser Confocal Microscope Leica TCS SP8 X

The white light laser source of the Leica TCS SP8 X perfectly matches the wavelength of any fluorophore. Up to eight excitation lines can be used – simultaneously. By tuning both excitation and detection, complete excitation emission spectra can be acquired.

With this spectral information, any dye can be optimally excited with minimum cross-excitation and specimen damage. In addition, the pulsed White Light Laser (WLL) supplies excitation light for fluorescence lifetime measurements.

FLIM provides information about the direct environment of a molecule, like local pH or binding to another molecule.

High-resolution Spectral Confocal for Daily Research and Routine Examinations Leica TCS SPE

The Leica TCS SPE confocal is a true point-scanning, spectral system at an affordable price for fluorescence imaging of live or fixed cells.

Providing all of the features needed for routine confocal techniques,it offers excellent quality imaging. The system is easy to use, and first results are quickly achieved, even by confocal novices.  The common Leica LAS X interface facilitates direct operation.

With its small footprint, the Leica TCS fits in every laboratory at common room conditions. The Leica TCS SPE is the only confocal in its class that provides true spectral detection suitable for lambda scan.

Hybrid Detector Leica HyD

Photon counting or imaging? Low light or bright fluorescence? High speed or crisp images? You don‘t need to compromise. The Leica HyD hybrid detector for confocal imaging with the Leica TCS SP8 offers unparalleled contrast for brilliant, publication-ready images out of the box. The Leica HyD sets the standard for super-sensitive imaging.

High Content Screening Automation Leica HCS A

Breakthrough discoveries can happen when you are in the right place at the right time. Leica HCS A can speed up the process of discovery through high content screening automation, HCS A. The integration of Leica HCS A into the confocal and widefield systems of Leica Microsystems allows you to standardize biological applications for rapid and reproducible results.

Combine the flexibility of a point-scanning confocal with the high speed of a camera-based widefield system in the same assay to save time. Compensation for specimen drift, single object tracking and immersion fluid are taken care of for high quality raw data.

Leica HCS A supports microscope platforms of Leica Microsystems: Leica TCS SP8, Leica TCS SPE and LAS X Widefield Systems.

Regardless of which platform you choose Leica HCS A can mark the start of understanding of your biological question, too.

Motorized Correction Collar for the Best Optical Performance in 3D Leica motCORR Objectives

The motorized correction collar of the Leica motCORR™ objectivessimplifies the workflow by quickly adjusting the objective lenses to varying coverglass thickness, changes in temperature, and specimen inhomogeneities.

The optical performance of high numerical aperture water immersion objectives is highly sensitive to refractive index mismatches caused by such variations. Precise adjustment of the correction collar ensures that optimal resolution, signal intensity and penetration depth is restored.

Leica motCORR™ objectives provide superior color correction and field flatness based on Leica’s Plan Apochromatic (PL APO) optics for the best imaging results.

3D Optical Surface Metrology System Leica DCM8

Designed to help you maximize efficiency, Leica DCM8 unites the advantages of High Definition confocal microscopy with interferometry into one versatile, dual-core system. Ultra-fast analysis is ensured thanks to one-click mode selection, sophisticated software and HD confocal scanning without moving parts.

Smart Monitoring Option RemoteCare

RemoteCare is a smart monitoring and service option for your microscope. It tracks the most important operating conditions and indicates early if something is off from the standard or expected result.

  • This smart monitoring prevents downtimes and ensures that the instrument is up and running.
  • If a service case happens to occur, your Leica Service team can solve it remotely in more than 30% of the cases thanks to information made readily available by RemoteCare.
  • For all cases, the documented data gives us the relevant information needed to get the instrument back in operation quickly.

RemoteCare is safe. It utilizes state-of-the-art Device Relationship Management technology to securely communicate in real time with Leica’s Remote monitoring platform across an encrypted internet connection.

RemoteCare transmits only diagnostic information about the instrument which helps us to provide you with faster service.

Leica Microsystems

Leica Microsystems develops and manufactures microscopes and scientific instruments for the analysis of microstructures and nanostructures.

Widely recognized for optical precision and innovative technology, the company is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning and super-resolution microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopy.

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