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Microcalorimeters for characterization of biomolecular stability and interactions
MicroCal microcalorimeters are powerful, analytical tools used to study biological systems. These include interactions between molecules as well as changes in conformation such as protein unfolding. The technique is very convenient requiring only minimal assay development and NO labelling. The range includes both Differential Scanning and Isothermal Titration calorimetry.
MicroCal microcalorimeters are used in a wide range of applications from basic research to the discovery and development of small molecule drugs, biotherapeutics and vaccines. Our customers include pharmaceutical, biotech, academic and governmental research institutions worldwide.
MicroCal DSC microcalorimeters are powerful analytical tools for characterizing the stability of proteins and other biomolecules, requiring minimal assay development, no labelling or immobilization. The automation and high sensitivity afforded by MicroCal VP-Capillary DSC systems permits screening of large numbers of samples at low concentrations minimizing the consumption of valuable samples with little hands-on effort.
MicroCal ITC isothermal titration calorimeters are used to study a wide range of biomolecular interactions. The applications range from drug design to fundamental research such as the understanding and regulation of signal transduction pathways. These systems deliver direct, label free and in-solution measurements of binding affinity and thermodynamic parameters in a single experiment. They have high sensitivity, low sample consumption and options for automation to minimize ‘hands on’ time. A choice of instruments ensures that MicroCal ITC calorimeters meet the varied and demanding requirements of today’s life science laboratories.
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Technical Specifications
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Measurement
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Temperature midpoint,Enthalpy,Heat capacity change
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Temperature range
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-10°C to 130°C
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Sample capacity
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576 (six 96 well plates)
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Throughput
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50 samples / 24 h (automated)
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Sample volume
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370 µL
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Cell volume
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130 µL
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Cell material
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Tantalum
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Noise
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0.05 µCal/°C
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Typical Concentration
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0.1 - 2.0 mg/mL
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Maximum scan rate
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240 ºC/h
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Response time
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5 s
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Baseline repeatability
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1.5 µCal/°C
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The MicroCal VP-Capillary DSC is a highly sensitive, fully automated, high-throughput differential scanning calorimeter for characterizing protein and biomolecule stability. The resulting data provide critical guidance for biopharmaceutical development, from protein engineering, through pre-formulation and process development, to formulation of the final product. Unattended operation enables 24-hour working while integrated software streamlines workflow and data analysis, delivering results in hours and driving productivity in biopharmaceutical research.
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Technical Specifications
|
|
|---|---|
|
Measurement
|
Temperature midpoint,Enthalpy,Heat capacity change
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|
Temperature range
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-10°C to 130°C
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Sample capacity
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2 - 5 samples / 8 h (manual)
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Sample volume
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700 µL
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Cell volume
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500 µL
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Cell material
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Tantalum
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Noise
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0.25 µCal/°C
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Typical Concentration
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0.1 - 2.0 mg/mL
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Maximum scan rate
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90 ºC/h
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Response time
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7 s
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Baseline repeatability
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1.25 µCal/°C
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The MicroCal VP-DSC is a highly sensitive, easy-to-use differential scanning calorimeter for the study of samples in solution. It is used to directly measure the intramolecular stability of structured macromolecules such as proteins and nucleic acids as well as the intermolecular stability of complexes such as oligomeric proteins, nucleic acid duplexes and lipid and detergent micellar systems.
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Technical Specifications
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|---|---|
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Measurement
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Enthalpy,Affinity,Entropy,Stoichiometry
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Temperature range
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2°C to 80°C
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Sample capacity
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4 - 8 samples / 8 h
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Sample volume
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2 mL
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Cell volume
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1400 µL
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Injection syringe volume
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300 µL
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Cell material
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Hastelloy
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Noise
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0.05 nCal/°C
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Response time
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20 s
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The MicroCal VP-ITC is a powerful and sensitive, easy-to-use isothermal titration calorimeter that offers the ability to investigate any biomolecular interactions. This fast, convenient, label free and in-solution technique delivers the information needed to characterize the molecular interactions of proteins, antibodies, nucleic acids and other biomolecules. The system is widely used in research environments for lead optimization, hit validation, assessing the driving mechanisms of binding and studying enzyme kinetics and inhibitor design.
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Technical Specifications
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|
|---|---|
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Measurement
|
Enthalpy,Affinity,Entropy,Stoichiometry
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Temperature range
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2°C to 80°C
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Temperature stability
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± 0.00015°C
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Sample capacity
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8 - 12 samples / 8 h
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Sample volume
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280 µL
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Cell volume
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200 µL
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Injection syringe volume
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40 µL
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Injection volume precision
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<1% @ 2µL
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Equilibrium time
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< 6 min (from 25°C to 5°C)
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Cell material
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Hastelloy
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Noise
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0.2 ncal/s
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Response time
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10 s
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The MicroCal iTC200 is a highly sensitive, low volume isothermal titration calorimeter for the label-free in solution study of biomolecular interactions. It delivers direct measurement of all binding parameters in a single experiment and can analyze weak to high affinity binders, using as little as 10 µg sample. Semi automated maintenance minimizes operator intervention and the system is upgradable to the fully automated MicroCal Auto-iTC200, making it ideal for laboratories where speed, sensitivity and the ability to accommodate higher workloads in the future are paramount.
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Technical Specifications
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|---|---|
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Measurement
|
Enthalpy,Affinity,Entropy,Stoichiometry
|
|
Temperature range
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2°C to 80°C
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Temperature stability
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± 0.00012°C
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Sample capacity
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384 (four 96 well plates)
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Sample volume
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370 µL
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Cell volume
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200 µL
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Injection syringe volume
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40 µL
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Injection volume precision
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<1% @ 2µL
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Throughput
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Up to 42 per 24 hr
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Cell material
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Hastelloy
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Noise
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0.15 ncal/s
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Response time
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8 s
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The MicroCal PEAQ-ITC Automated is a configurable, low volume, highly sensitive isothermal titration calorimeter which offers the convenience of unattended operation. It delivers direct, label-free in solution measurement of all binding parameters in a single experiment. Applications include characterizing molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules. It can also be used to measure enzyme kinetics
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Technical Specifications
|
|
|---|---|
|
Measurement
|
Enthalpy,Affinity,Entropy,Stoichiometry
|
|
Temperature range
|
2°C to 80°C
|
|
Temperature stability
|
± 0.00012°C
|
|
Sample capacity
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384 (four 96 well plates)
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|
Sample volume
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280 µL
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Cell volume
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200 µL
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Injection syringe volume
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40 µL
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Injection volume precision
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<1% @ 2µL
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Throughput
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8-12 per 8 hr
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Cell material
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Hastelloy
|
|
Noise
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0.15 ncal/s
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Response time
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8 s
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The MicroCal PEAQ-ITC Automated is a configurable, low volume, highly sensitive isothermal titration calorimeter which offers the convenience of unattended operation. It delivers direct, label-free in solution measurement of all binding parameters in a single experiment. Applications include characterizing molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules. It can also be used to measure enzyme kinetics
Measurements of binding affinity and thermodynamic parameters in a single experiment
High sensitivity with options for automation to minimize ‘hands on’ time
Low sample consumption
Malvern Instruments MicroCal DSC team introduce what Differential Scanning Calorimetry (DSC) is and why it is the gold standard in characterizing the stability of a protein or other biomolecules.
The Malvern MicroCal team details how Differential Scanning Calorimetry (DSC) works and why you should be using it for testing protein stability.
Malvern Panalytical was formed by the merger of Malvern Instruments Limited and PANalytical B.V. on 1st January 2017, has headquarters in both Almelo (the Netherlands), and in Malvern (UK), and employs over 2,000 people worldwide. The combined entity is a strong player and innovator in the materials characterization market and will leverage the strengths of the individual companies in their end markets, ranging from building materials to pharmaceuticals and from metals and mining to nanomaterials.
