Microcalorimeters for characterization of biomolecular stability and interactions
MicroCal microcalorimeters are powerful, analytical tools used to study biological systems. These include interactions between molecules as well as changes in conformation such as protein unfolding. The technique is very convenient requiring only minimal assay development and NO labelling. The range includes both Differential Scanning and Isothermal Titration calorimetry.
MicroCal microcalorimeters are used in a wide range of applications from basic research to the discovery and development of small molecule drugs, biotherapeutics and vaccines. Our customers include pharmaceutical, biotech, academic and governmental research institutions worldwide.
MicroCal ITC isothermal titration calorimeters are used to study a wide range of biomolecular interactions. The applications range from drug design to fundamental research such as the understanding and regulation of signal transduction pathways. These systems deliver direct, label free and in-solution measurements of binding affinity and thermodynamic parameters in a single experiment. They have high sensitivity, low sample consumption and options for automation to minimize ‘hands on’ time.
The MicroCal PEAQ-ITC Automated is a configurable, low volume, highly sensitive isothermal titration calorimeter which offers the convenience of unattended operation. It delivers direct, label-free in solution measurement of all binding parameters in a single experiment. Applications include characterizing molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules.
The MicroCal PEAQ-ITC is a highly sensitive, low volume isothermal titration calorimeter for the label-free in solution study of biomolecular interactions. It delivers direct measurement of all binding parameters in a single experiment and can analyze weak to high affinity binders, using as little as 10µg sample. Semi-automated maintenance minimizes operator intervention and the system is upgradable to the fully automated MicroCal PEAQ-ITC Automated, making it ideal for laboratories where speed, sensitivity and the ability to accommodate higher workloads in the future are paramount.
MicroCal DSC microcalorimeters are powerful analytical tools for characterizing the stability of proteins and other biomolecules, requiring minimal assay development, no labelling or immobilization. The automation and high sensitivity afforded by MicroCal VP-Capillary DSC systems permits screening of large numbers of samples at low concentrations minimizing the consumption of valuable samples with little hands-on effort.
The high-throughput, high-sensitivity MicroCal PEAQ-DSC Automated system features software that streamlines workflows through simplified experimental setup and flexible instrument scheduling. Automated data analysis supports the generation of high integrity thermal stability data and delivers compliance with regulatory requirements, whilst allowing easy integration into existing data handling and transfer systems.
The high-sensitivity MicroCal PEAQ-DSC system features automated data analysis to support the generation of high integrity thermal stability data and deliver compliance with regulatory requirements, whilst allowing easy integration into existing data handling and transfer systems. Unattended operation of the instrument after sample loading frees operator time. This complete system requires no additional accessories, reagents or consumables.
Technical Specifications
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Measurement
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Enthalpy,Affinity,Entropy,Stoichiometry
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Temperature range
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2°C to 80°C
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Temperature stability
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± 0.00012°C
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Sample capacity
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384 (four 96 well plates)
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Sample volume
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370 µL
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Cell volume
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200 µL
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Injection syringe volume
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40 µL
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Injection volume precision
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<1% @ 2µL
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Throughput
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Up to 42 per 24 hr
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Cell material
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Hastelloy
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Noise
|
0.15 ncal/s
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Response time
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8 s
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The MicroCal PEAQ-ITC Automated is a configurable, low volume, highly sensitive isothermal titration calorimeter which offers the convenience of unattended operation. It delivers direct, label-free in solution measurement of all binding parameters in a single experiment. Applications include characterizing molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules. It can also be used to measure enzyme kinetics
Technical Specifications
|
|
---|---|
Measurement
|
Enthalpy,Affinity,Entropy,Stoichiometry
|
Temperature range
|
2°C to 80°C
|
Temperature stability
|
± 0.00012°C
|
Sample capacity
|
384 (four 96 well plates)
|
Sample volume
|
280 µL
|
Cell volume
|
200 µL
|
Injection syringe volume
|
40 µL
|
Injection volume precision
|
<1% @ 2µL
|
Throughput
|
8-12 per 8 hr
|
Cell material
|
Hastelloy
|
Noise
|
0.15 ncal/s
|
Response time
|
8 s
|
The MicroCal PEAQ-ITC Automated is a configurable, low volume, highly sensitive isothermal titration calorimeter which offers the convenience of unattended operation. It delivers direct, label-free in solution measurement of all binding parameters in a single experiment. Applications include characterizing molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules. It can also be used to measure enzyme kinetics
Technical Specifications
|
|
---|---|
Sample capacity
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288 (6 × 96-well plates)
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Sample volume
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325 µL
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Typical sample concentration
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0.01 mg/mL – 10 mg/mL 1
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Sample throughput
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≤50 samples/day
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Sample storage temperature range
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4°C - 40°C
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Operating temperature (°C)
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+10°C to +28°C
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Storage temperature
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-20°C to +50°C
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Humidity
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10% to 70%, non-condensing (10% to 90% for storage)
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Ingress Protection (IP) rating
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IP21
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Power
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100-240 V A/C, 50/60 Hz, 70 W (cell), 400 W (max, autosampler), PC as supplied
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Certification
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CE (EN61010-1), EMC (EN61326-2-1, EN61326-1, FCC, ICES, VCCI), ISO9001:2008
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Dimensions (W x D x H)
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101 cm × 68 cm × 70 cm
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Weight
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Approx. 25 kg
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Gold standard protein stability analysis for the regulated environment
The MicroCal PEAQ-DSC Automated system delivers high throughput, highly sensitive protein stability analysis with low sample consumption, in an automated, integrated platform for increased productivity. All cell filling and cleaning functions are fully automated for walk-away operation.
Key Features
Technical Specifications
|
|
---|---|
Sample volume
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250 µL (manual filling)
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Typical sample concentration
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0.01 mg/mL – 10 mg/mL 1
|
Sample throughput
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≤6 samples analyzed/8h
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Operating temperature (°C)
|
+10°C to +28°C
|
Storage temperature
|
-20°C to +50°C
|
Humidity
|
10% to 70%, non-condensing (10% to 90% for storage)
|
Ingress Protection (IP) rating
|
IP21
|
Power
|
100-240 V A/C, 50/60 Hz, 70 W (cell), PC as supplied
|
Certification
|
CE (EN61010-1), EMC (EN61326-2-1, EN61326-1, FCC, ICES, VCCI), ISO9001:2008
|
Dimensions (W x D x H)
|
20 cm × 44 cm × 19 cm
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Weight
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8.2 kg
|
|
1 Sample dependent
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Gold standard protein stability analysis for research applications
The MicroCal PEAQ-DSC system provides highly sensitive, easy-to-use microcalorimetry that helps reduce the time and cost associated with stability testing and comparability analysis. The MicroCal PEAQ-DSC is a manual instrument with a cleaning device, which can be upgraded to an automated version upon request.
Key Features
Measurements of binding affinity and thermodynamic parameters in a single experiment
High sensitivity with options for automation to minimize ‘hands on’ time
Low sample consumption
Malvern Instruments MicroCal DSC team introduce what Differential Scanning Calorimetry (DSC) is and why it is the gold standard in characterizing the stability of a protein or other biomolecules.
The Malvern MicroCal team details how Differential Scanning Calorimetry (DSC) works and why you should be using it for testing protein stability.