Scalable Single-Use Centrifugation Systems
Sartorius patented Ksep® technology is the only current technology that provides significant advantages for users that want to either harvest cells as product or separate cells and debris-free media that contains the product.
Through the balance of centrifugal and fluid flow forces, the Ksep® retains particles, such as cells or microcarriers, as a concentrated fluidized bed under a continuous flow of media or buffer. Ksep® systems are currently being used by top biopharmaceutical companies for diverse bioprocessing applications.
Our current offerings include three models, designed to handle low to very high cell densities and with built-in scalability ranging from 0.1 L to 2000 L. Our high-quality, disposable sets are manufactured using class VI materials and gamma irradiated.
Ksep®400 | Ksep®6000S | |
Max g-force | 1,000 g | 2,000 g |
Max Flow Rate | 114 L/hr | 720 L/hr |
Volume | 400 mL (4 × 100 mL) | 6000 mL (6 × 1,000 mL) |
Cell Capacity per Cycle (CHO) | Up to 80 Billion | Up to 1200 Billion |
Processing Volumes | 0.1 –500 L | 10–2,000 L |
Height | 140 cm | 179 cm |
Length | 107.5 cm | 225 cm |
Width | 72 cm | 106 cm |
Weight | 350 kg | 2141 kg |
Voltage | 208–240 V 1Ph | 208–240 V 3Ph (US) | 400 V 3Ph (EU) |
Current | 20 A | 60 A |
Process Air | Not required | 90 psi – ½” NPT |
Connection | NEMA L6–20 | Customer supplied |
Chilled Water (Optional) | ½” NPT | ¾” NPT |
Ksep® 400 concentrate cells with high recovery while maintaining high viability. Additionally, Ksep® systems can remove cell debris, light particulate impurities, all while significantly reducing any aggregation of cells.
Ksep® systems do not contain any rotary seals (providing completely closed system) or filters (for reduced issues from clogging). These features are critical for cell therapy manufacturing. Once captured and concentrated, the cells can efficiently be washed, manipulated, and harvested.
Ksep® is a breakthrough for applications requiring maintenance of cellular integrity during processing. This automated sequence is currently being used for cell therapy manufacturing, perfusion, cell banking, and vaccine manufacturing processes. This is the only perfusion technology where the bleeding of cells does not cause loss of recovery.
Ksep® 6000S concentrate cells with high recovery while maintaining high viability. Additionally, Ksep® systems can remove cell debris, light particulate impurities, all while significantly reducing any aggregation of cells.
Ksep® systems do not contain any rotary seals (providing completely closed system) or filters (for reduced issues from clogging). These features are critical for cell therapy manufacturing. Once captured and concentrated, the cells can efficiently be washed, manipulated, and harvested.
Ksep® is a breakthrough for applications requiring maintenance of cellular integrity during processing. This automated sequence is currently being used for cell therapy manufacturing, perfusion, cell banking, and vaccine manufacturing processes. This is the only perfusion technology where the bleeding of cells does not cause loss of recovery.