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Scalable Single-Use Centrifugation Systems

Sartorius patented Ksep® technology is the only current technology that provides significant advantages for users that want to either harvest cells as product or separate cells and debris-free media that contains the product.

Through the balance of centrifugal and fluid flow forces, the Ksep® retains particles, such as cells or microcarriers, as a concentrated fluidized bed under a continuous flow of media or buffer. Ksep® systems are currently being used by top biopharmaceutical companies for diverse bioprocessing applications.

Our current offerings include three models, designed to handle low to very high cell densities and with built-in scalability ranging from 0.1 L to 2000 L. Our high-quality, disposable sets are manufactured using class VI materials and gamma irradiated.

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Technical Specification

Ksep®400 Ksep®6000S
Max g-force 1,000 g 2,000 g
Max Flow Rate 114 L/hr 720 L/hr
Volume 400 mL (4 × 100 mL) 6000 mL (6 × 1,000 mL)
Cell Capacity per Cycle (CHO) Up to 80 Billion Up to 1200 Billion
Processing Volumes 0.1 –500 L 10–2,000 L
Height 140 cm 179 cm
Length 107.5 cm 225 cm
Width 72 cm 106 cm
Weight 350 kg 2141 kg
Voltage 208–240 V 1Ph 208–240 V 3Ph (US) | 400 V 3Ph (EU)
Current 20 A 60 A
Process Air Not required 90 psi – ½” NPT
Connection NEMA L6–20 Customer supplied
Chilled Water (Optional) ½” NPT ¾” NPT

Available models

Ksep® 400 concentrate cells with high recovery while maintaining high viability. Additionally, Ksep® systems can remove cell debris, light particulate impurities, all while significantly reducing any aggregation of cells.

Ksep® systems do not contain any rotary seals (providing completely closed system) or filters (for reduced issues from clogging). These features are critical for cell therapy manufacturing. Once captured and concentrated, the cells can efficiently be washed, manipulated, and harvested.

Ksep® is a breakthrough for applications requiring maintenance of cellular integrity during processing. This automated sequence is currently being used for cell therapy manufacturing, perfusion, cell banking, and vaccine manufacturing processes. This is the only perfusion technology where the bleeding of cells does not cause loss of recovery.

Ksep® 6000S concentrate cells with high recovery while maintaining high viability. Additionally, Ksep® systems can remove cell debris, light particulate impurities, all while significantly reducing any aggregation of cells.

Ksep® systems do not contain any rotary seals (providing completely closed system) or filters (for reduced issues from clogging). These features are critical for cell therapy manufacturing. Once captured and concentrated, the cells can efficiently be washed, manipulated, and harvested.

Ksep® is a breakthrough for applications requiring maintenance of cellular integrity during processing. This automated sequence is currently being used for cell therapy manufacturing, perfusion, cell banking, and vaccine manufacturing processes. This is the only perfusion technology where the bleeding of cells does not cause loss of recovery.

  • Biopharmaceutical and Biotechnology
Sartorius Lab InstrumentsThe Sartorius Group is a leading international partner of the biopharmaceutical industry and the research sector. We are helping biotech scientists and engineers across the entire globe to develop and manufacture medications from the first idea to production. So more people will have access to better medicine.
[Sartorius, harvest, harvest cell, separation, centrifuge, centrifugation]