Waters - LC Columns

XBridge Premier Columns

Particle Stability with the Protection of MaxPeak HPS

XBridge Premier 2.5 µm Columns are the evolution of Waters XBridge 2.5 µm XP (eXtended Performance) columns. They combine the industry leading column stability of Ethylene Bridged Hybrid (BEH) particles with MaxPeak High Performance Surface (HPS) Technology. XBridge Premier Columns are available in a standard column format or a VanGuard FIT (Fully Integrated Technology) column format. The VanGuard FIT Column is an all-in-one guard and column design that’s easy-to-use, and extends column lifetimes when analyzing samples in challenging matrices.

XBridge Premier 2.5 µm Columns are part of the MaxPeak Premier Class of columns. Designed to be the most universal column platform, XBridge Premier Columns prevents undesired analyte/surface interactions by utilizing MaxPeak HPS Technology. MaxPeak HPS is designed to increase analyte recovery, sensitivity, and reproducibility by minimizing analyte/surface interactions that can lead to sample losses. In the MaxPeak Premier Class of columns, non-specific adsorption is dramatically reduced for analytes that may bind to metal surfaces, without sacrificing performance for those that do not.

With a reduction in these undesired interactions, you can expect:

• Reduced column conditioning and passivation, leading to higher productivity
• Improved sensitivity and peak shapes, leading to better quality data
• Increased reproducibility from analysis to analysis, leading to more confidence in analytical results

nanoACQUITY UPLC Columns

nanoACQUITY Trap and Separation Columns

nanoACQUITY Columns and trap columns are the previous generation of columns for nano- and microflow LC-MS applications. They are being replaced by the new generation of ACQUITY UPLC M-Class columns:

• Higher pressure rating (15,000 psi/1,034 bar) enable higher flow rates and thus shorter run times and higher sample turnover.
• M-Class Columns are compatible with nanoACQUITY UPLC systems.

XBridge BEH XP Columns

Universal Column Family

The common base particle for all XBridge BEH HPLC, eXtended Performance (XP) and ACQUITY UPLC Columns provide seamless scalability throughout the available particle sizes (1.7, 2.5, 3.5, 5, and 10 μm). Improve existing HPLC methods 4-fold by selecting a column configuration that provides the correct balance between resolution and sample throughput.

XBridge BEH XP Columns

Universal Column Family

The common base particle for all XBridge BEH HPLC, eXtended Performance (XP) and ACQUITY UPLC Columns provide seamless scalability throughout the available particle sizes (1.7, 2.5, 3.5, 5, and 10 μm). Improve existing HPLC methods 4-fold by selecting a column configuration that provides the correct balance between resolution and sample throughput.

Symmetry HPLC Columns

Silica-based columns that exceed HPLC column performance expectations

Reproducible HPLC columns are essential when developing analytical methods for pharmaceutical and biopharmaceutical products. Achieving the same chromatographic results over the lifetime of a new drug product is critical, and no other silica-based LC column brand can match the column-to-column and batch-to-batch reproducibility of the Symmetry family.

Available in column and guard formats, Symmetry Columns are manufactured using high purity silica and tightly controlled manufacturing processes to ensure that you receive a column that exceeds the standards for HPLC column performance.

Atlantis Columns

Reversed-phase and HILIC HPLC Columns for superior polar compound retention

Polar analytes are difficult to retain using traditional reversed-phase (RP) chromatography as they often elute in the void volume of the column. You can solve this challenge today with Atlantis Columns, which retain and separate polar analytes under reversed phase and HILIC conditions and provide quick, accurate, and reproducible quantification with increased retention and sensitivity.

Available in T3, dC18, and the unbonded HILIC chemistries, the Waters Atlantis Column family provides exceptional performance, versatility, retention, and chromatographic stability for polar compounds and balanced retention for broad analyte mixtures. 

SunFire Columns

Superior mass loading at low pH

High efficiency at a low pH can be challenging. Waters SunFire HPLC Columns, available in analytical and preparative dimensions, help you do just that with state-of-the-art bonded silica designed for high mass loading, excellent low pH stability, superior peak shapes, and high efficiency.

SunFire Analytical Columns are available in 2.5 µm to 10 µm particle sizes across two reversed-phase column chemistries, C18 and C8, sure to meet your silica-based HPLC column requirements. For preparative applications, in addition to the C18 and C8 chemistries, SunFire silica is offered in 5 and 10 µm particle sizes for preparative normal-phase purification.

Nova-Pak Columns

Scale-up from analytical to prep, simplified

Reducing solvent consumption while maintaining resolution for complex mixtures is important, but that’s a challenge with larger particle sizes. With the smaller particle sizes and shorter column lengths in Nova-Pak Columns, those problems are a thing of the past. The bonded phases of Nova-Pak Columns are available in 4 µm and 6 µm particle sizes, offering high resolution and faster, more efficient chromatography over columns packed with larger particle sizes.

Nova-Pak Analytical Columns with 4 µm particle size packing offer a range of length options with 75, 100, 150, and 300 mm length steel columns. The semi-preparative Prep Nova-Pak HR Columns are packed with 6 µm particle size packings for unparalleled separation possibilities. The high-efficiency packing of Prep Nova-Pak HR Columns provides faster separations with less solvent for the added advantage of more concentrated fractions—reducing your preparative chromatography cost. To ensure batch-to-batch reproducibility, all Nova-Pak C18, C8, Cyano and Phenyl Columns are packed according to stringent quality control (QC) procedures in Waters cGMP manufacturing facility.

Waters Spherisorb HPLC Columns 

HPLC Columns for diverse separation selectivities

When you’re developing your methods, you don’t want to leave anything to chance – especially whether your columns will work. That’s not a problem with Waters Spherisorb Columns, one of the most widely referenced HPLC columns in scientific literature. With more than 2,000 analytical abstracts published using these columns, Spherisorb Columns provide a tremendous range of validated methods and applications to assist in your method development process.

And with a wide range of particle sizes (3, 5, and 10 µm) and high-quality bonded phases for different and unique separation selectivities, there’s sure to be a Waters Spherisorb Column to meet your analytical and preparative column chromatography needs. 

Resolve Columns

High resolution spherical packings

Non-endcapped Resolve packings are significantly different from Waters other packing materials in that they typically provide higher retention of polar compounds and complement those of Nova-Pak and µBondapak chemistries. Resolve C18 and silica columns are available in 5 µm and 10 µm spherical packings for applications requiring higher resolution than what is achievable using irregularly shaped chromatographic particles. The non-endcapped silica surface offers a balance of retention for separation of polar compounds.

µBondapak and Bondapak Columns

The original µBondapak C18 packing material

Scale from analytical to prep and separate acidic, basic, and neutral compounds with µBondaPak and BondaPak Columns, the only columns with the original µBondaPak C18 packing material.

While many companies may claim “μBondapak-like” selectivity, none have passed Waters stringent quality control (QC) batch tests. By passing these tests, μBondapak and BondaPak packing materials have ensured reproducibility for Waters customers year after year since 1973 — becoming one of the most widely referenced HPLC column brands.

µPorasil/Porasil Columns

Normal-phase separations for a wide array of sample types

μPorasil and Porasil particles were one of the first commercially available, fully porous packing materials used for LC separations. In contrast to the reversed-phase separation ability of μBondapak C18, the non-bonded, silica-based material in μPorasil Columns was produced to provide normal-phase separations for a wide array of sample types.

With a range of different particle size offerings, you have the option of scalability for preparative application with µPorasil column as a QC tool.

Waters PAH Columns

Improved Analysis of PAH Compounds

Polyaromatic hydrocarbons (PAHs) are among the most frequently monitored environmental contaminants. Standard and official methods for the analysis of PAHs are found in compendia for air, drinking water, wastewater, solid waste, and food analysis. Many of these methods specify HPLC, usually with UV and fluorescence detection, as the recommended analytical procedure. Waters PAH columns are optimized for the HPLC analysis of PAHs. These columns achieve baseline resolution and excellent peak symmetry for 16 different target analytes in less than 25 minutes, while employing a simple water/acetonitrile binary gradient method. The resolving power of the PAH columns provides superior peak shape identification and quantitation for PAHs. They come in seven different dimensions (including a capillary format) and two particle sizes.

Protein-Pak Columns

Analysis and lab scale isolation of proteins or polypeptides

Protein-Pak LC Columns contain either well-performing silica or methacrylate-based, ion-exchange, size-exclusion, and hydrophobic interaction, and affinity column chemistries for the separation, lab-scale purification, and characterization of proteins and other biomolecules.

The Protein-Pak HPLC and UPLC Column family includes:

• Hi Res HIC columns
• Hi Res ion-exchange (IEX) columns
• Ion-exchange glass columns
• Affinity epoxy-activated columns

AccQ•Tag Ultra, AccQ•Tag and Pico•Tag Columns

UPLC, UHPLC, and HPLC-Based Methods for Accurate Amino Acid Composition Analysis

Waters AccQ•Tag and Pico•Tag technologies enable your laboratory to obtain accurate amino acid composition from protein/peptide hydrolysates, physiologic fluids, feeds, foods, pharmaceutical preparations, and myriad other samples using traditional HPLC or UHPLC  instrumentation. The Waters AccQ•Tag Ultra Column is designed for use on Waters ACQUITY UPLC platforms to deliver superior amino acid component resolution and in less time compared to use of more traditional HPLC or UHPLC methods.

AccQ•Tag and Pico•Tag products include:

• Pre-column derivatization reagents
• Dedicated reversed-phase columns QC tested in application
• HPLC / UHPLC or UPLC based eluents and standards
• Documented methods

Amino Acid Analysis

Amino Acid Analysis

Determination and control of critical nutrients in cell culture is important to maximize protein yield and provide a consistent manufacturing process. The Waters UPLC^®Amino Acid Analysis (AAA) Solution is a turnkey solution that provides accurate, high throughput amino acid analysis.

The UPLC Amino Acid Analysis Solution meets the specific demands of analyzing amino acids for protein characterization, cell culture monitoring, and nutritional analysis of foods and feeds. This end-to-end system is designed to deliver the accuracy and reliability you need to be successful.

HILIC for Released Glycans

Robust LC or LC-MS Analyses of Biotherapeutic Glycoproteins

The analysis of complex N- and 0-linked glycans composed of frequently similar and repeating sugar moieties requires a highly discriminating chromatographic method. Hydrophilic liquid interaction chromatography (HILIC), using UPLC, HPLC, or UHPLC columns containing Waters amide bonded, Ethylene Bridged Hybrid (BEH) Technology particles separates compounds based on differing hydrophilicity.

Sialic Acid and Monosaccharide Analyses

Orthogonal Techniques for Quantitation

Evidence for reliable and consistent glycosylation of glycoprotein therapeutics is typically obtained through LC or LC-MS analysis of released N-linked glycans. However, drug discovery and development organizations, as well as regulatory agencies, frequently find value using orthogonal techniques to determine and/or confirm quantitative levels of sialic acids and monosaccharides contained in a therapeutic protein due to potential effect on biological activity and stability of the drug.

Waters offers UPLC, UHPLC, and HPLC-based C18 columns and defined application conditions that can assist scientists with the analysis and quantitation of these important glycan derived components.

Glycan Standard & Kits

Specially formulated and high-quality

Waters Glycan Standards and Kits are for released N-glycan labeling.  We offer standards in a wide variety of labels, 2-AB, 2-AA and RapiFluor-MS.  In addition, we offer complete kits for both 2-AB and RapiFluor-MS labeling in a variety of  sample sizes for low throughput to high throughput.

Automation Workflow Kits and Scripts

Realize the full potential of your automation workstations with Waters sample preparation kits. Compatible with a variety of industry-standard platforms, seamlessly automate your Waters workflow to gain laboratory efficiency without encountering the issues inherent in scripting complex manual methods. 

• Transition to routine, high-throughput analysis with Waters easily customizable, application-specific base scripts for Andrew Alliance, Hamilton, and Tecan platforms
• Automation-ready application kits designed for various automation platforms
• Verified platform scripts and detailed user guides to ensure seamless deployment and robust analytical method performance 

Enzymate Pepsin Column

Faster, more efficient online protein digestion

Fast, efficient, and reproducible online pepsin digestion is critical in experiments that require more peptide coverage. With the Waters Enzymate BEH Pepsin Column, you can easily incorporate online protein digestion into existing HDX- MS workflows.

The Enzymate BEH Pepsin Column efficiently digests intact proteins into peptides in an online HDX system, typically in about 30 seconds. The peptic peptides are then retained on a trapping column and refocused onto a sub-2-μm ACQUITY UPLC Column for elution into high-resolution mass spectrometry.

MassPREP™ on-line desalting cartridges are an effective tool for desalting acidic, basic, and large globular proteins.

The MassPREP desalting strategy provides an easy, fast (less than 3 minutes), and reproducible approach for the effective desalting of proteins stored in physiological salt buffers prior to high resolution ESI-MS structural analysis.

• Effectively desalts proteins yielding improved ESI-MS results
• Fast on-line method for high-throughput applications
• Excellent protein recoveries and no detectable carryover
• More than 100 injections from a single cartridge

MassPREP Micro Desalting Cartridges

MassPREP micro desalting cartiridges contains a polymer-based, reversed-phase packing material for desalting protein samples prior to mass spectrometry. The phenyl-phase material in these on-line devices successfully retains proteins, allowing the salts to be washed to waste prior to elution of the desalted pro­tein into the mass spectrometer. With an optimized LC/MS method, you may have cycle times as low as 4 minutes.

Nano- and Microflow LC-MS Columns

Separation and Trap Columns

Waters nano- and microflow LC-MS columns are specifically designed for low-dispersion nano LC systems.

The selected stationary phases fully leverage the separation power of sub-2-µm particle technology. This delivers the efficiency and selectivity required for complex separations such as discovery of low abundance species, peptide mapping, protein identification, and biomarker discovery.

• Leverage the efficiency of sub-2-µm particle technology at high flow rates
• Stationary phases with a variety of characteristics suitable for biomolecule separations
• Easy interface with LC-MS systems

nanoACQUITY UPLC Columns

nanoACQUITY Trap and Separation Columns

nanoACQUITY Columns and trap columns are the previous generation of columns for nano- and microflow LC-MS applications. They are being replaced by the new generation of ACQUITY UPLC M-Class columns:

• Higher pressure rating (15,000 psi/1,034 bar) enable higher flow rates and thus shorter run times and higher sample turnover.
• M-Class Columns are compatible with nanoACQUITY UPLC systems.

Peptide Standards and Kits

Saving Valuable Time

Waters peptide standards and kits ensure productivity through reduction of instrument downtime.  These products also assist in the development, validation, and troubleshooting peptide applications.

• Consistent Chromatography:  Recommended to be used on a regular basis for monitoring column and system performance
• Quality:  Manufactured with strict quality control for batch-batch reproducibility
• Ease of Use: Packaged in Waters vials for direct solubilization

DNA/RNA Oligonucleotide Separations

Addressing Challenges in LC and LC-MS Based Analyses

Reversed-phase and ion-exchange chromatography for the analysis of synthetic oligonucleotides and related DNA/RNA species is well documented in their ability to purify and analyze these heterogeneous entities (e.g., full-length oligonucleotide product from undesired shorter and longer length sequences). With increased use of MS detection, Waters introduced the XTerra C18 family of "hybrid particles" that are MS compatible with increased high pH tolerance based on various application needs. Waters second-generation of XBridge C18 hybrid columns were introduced to expand performance characteristics.

• Scalable offering in 1.7 μm UPLC particles,  2.5 μm HPLC/UHPLC particles, and 5 µm lab-scale preparative particles in various column dimensions.
• Increased confidence in results: MaxPeak Premier Columns with MaxPeak HPS are available for those who have more challenging samples that are prone to metal affinity; providing improved sensitivity, recovery, reproducibility, and less time-consuming column conditioning. ACQUITY Premier and XBridge Premier Oligonucleotide Columns are available in 1.7 µm and 2.5 µm particle sizes.
• Exceptional sample resolution and superior column life.
• Confidence in results: All columns are QC tested with relevant application standards to help ensure consistent batch-to-batch and column-to-column performance.

Oligonucleotides Standards & Kits

Quality Control Reference Materials

Oligonucletotide separation columns deliver exceptional sample resolution and superior column life.  Now this chemistry has a powerful counterpart, application specific standards developed with the same methods used to run the column chemistry.  Designed in-house by our scientific experts, the standards in this portfolio are developed specifically with the column and system technologies.    

These standards are typically the same standards our quality control department uses to test the columns.  They are now offered directly to our customers to save valuable sample preparation and development time.

Peptide Reversed-Phase Columns

Reversed-Phase Chromatography

Reversed-phase chromatography using ion-pairing reagents such as TFA and formic acid can deliver highly resolved separations of complex peptide mixtures, (e.g., tryptic protein digests or lengthy synthetic peptide sequences) whose sequences may differ by a single amino acid. In general, the hydrophobicity of the peptide determines the elution order, with the least hydrophobic peptides eluting first.  

Factors such as particle composition (silica vs hybrids), pore size (130 Å vs 300 Å), ligand density, as well as separation conditions (e.g., gradient duration, separation temperature, flow rate) all play an important role in obtaining a separation that meets application requirements.

As with other bioseparations, when using smaller particle sizes, UPLC/UHPLC column offerings provide superior component resolution and in less time compared to HPLC-based columns for this demanding application.

Peptide Ion-Exchange Columns

Ion-Exchange Chromatography

Ion-exchange chromatography separates peptides based on charge differences and is used when a different / orthogonal separation mechanism is desired vs the more traditional use of  reversed-phase chromatography.

Separations are most commonly performed using a gradient of increasing salt concentration with the smaller, less charged peptide species eluting prior to the longer peptide fragments.   Unlike reversed-phase peptide separtions, eluent pH can significantly affect the separation so method development is required in order obtain satisfactory chromatography.

As with other bioseparations, use smaller particle size, UPLC/UHPLC offerings provide superior component resolution and in less time compared to our HPLC-based columns.

Peptide SEC Columns

Size-Exclusion Chromatography

Size exclusion, also known as gel filtration, chromatography separates proteins based on their sizes (hydrodynamic radii) in solution and not by absolute molecular weight with larger sized species eluting before smaller peptides.  The primary driving mechanism for this isocratic based technique is based on the pore size and volume of the SEC particles used in the packed column.   SEC columns containing comparatively larger pore size particles are selected as the molecular weight of the peptides of interest increase.

As with other bioseparations, use smaller particle size, UPLC/UHPLC offerings provide superior component resolution and in less time compared to our HPLC-based columns for this demanding application.

RapiGest SF Surfactant

Enhance the quality of in-solution enzymatic protein digestions

Labs around the globe are trying to simplify digestion protocols and improve throughput of analysis. Waters patented RapiGest SF Surfactant was designed with your lab’s efficiency in mind, significantly decreasing proteolytic digestion time, and enabling same-day digestions with mass spectrometry (MS) compatibility.

RapiGest SF radically enhances the speed and percent recovery of protein enzymatic digestions. This patented anionic surfactant accelerates the in-solution production of peptides generated by proteases, such as Trypsin, Asp-N, Glu-C, Lys-C, and PNGase F. Many hydrophobic proteins are resistant to proteolysis because their cleavage sites are inaccessible to endoproteases. RapiGest SF facilitates the digestion of proteolytically resistant proteins and increases the solubility of hydrophobic proteins and peptides. RapiGest SF is an MS-compatible mild protein denaturant that helps solubilize and unfold proteins, making them more amenable to cleavage without denaturing or inhibiting common proteolytic enzymes like conventional denaturants. This enables use of lesser amounts of expensive endoproteases.

Protein Standards

Specially Formulated

Waters Protein Standards and Protein Mixes are specially formulated and designed to aid in the evaluation and benchmarking of columns designed and used for protein separations. Waters line of Protein Standards provide:

• Consistent Chromatography:  Recommended to be used on a regular basis for monitoring column and system performance
• Quality:  Manufactured with strict quality control for batch-batch reproducibility
• Ease of Use:  Packaged in Waters Vials for direct solubilization

Oasis Sample Extraction Products

Faster, cleaner, and simpler sample preparation starts here

Laboratories worldwide would like simple sample preparation for robust method development. Get method development options with Waters Oasis sample extraction products, which come in a broad range of sorbents and an array of formats. With Oasis products, you can meet a wide variety of sample preparation needs, from simple and fast sample matrix clean-up to more complex sample preparation challenges.

Oasis Sample Extraction Products deliver unmatched purity, consistency, and quality for sample analysis. Available in seven different solid-phase extraction (SPE) chemistries, Oasis sorbents provide the range of options you need for method development.

Protein Ion-Exchange Columns

Ion-Exchange Chromatography

IEX separations are most commonly performed using gradients of increasing salt, changing pH, or simultaneous salt increases and pH changes with less charged protein species eluting prior to more highly charged molecules. Based on protein type and separation pH, either an anion or cation exchanger is selected for the separation. In addition, gradient duration, buffer composition and pH, flow rate, as well as separation temperature, all play an important part in obtaining desired protein separations.

Protein SEC Columns

Size-Exclusion Chromatography

Size-exclusion chromatography (SEC), also known as gel filtration, separates proteins based on their sizes (hydrodynamic radii versus absolute molecular weight) in solution with larger sized species eluting before smaller proteins. The primary driving mechanism for this isocratic based technique is based on the pore size and volume of the SEC particles used in the packed column. As the molecular weight of the proteins of interest increases, SEC columns containing comparatively larger pore size particles are selected. Why choose BEH SEC column technology?

• Less ionic interactions with stable Diol-coating for confidence in recovery and reproducibility
• Unlike silica-based particles, BEH is pH stable >pH 10 for method flexibility
• Outstanding column lifetime
• Scalable chemistries for method transfer
• Pore size options include 125 Å, 200 Å, and 450 Å depending on the protein of interest
• Synthesized batches are QC tested with relevant samples to help ensure consistency

Protein SEC Columns

Size-Exclusion Chromatography

Size-exclusion chromatography (SEC), also known as gel filtration, separates proteins based on their sizes (hydrodynamic radii versus absolute molecular weight) in solution with larger sized species eluting before smaller proteins. The primary driving mechanism for this isocratic based technique is based on the pore size and volume of the SEC particles used in the packed column. As the molecular weight of the proteins of interest increases, SEC columns containing comparatively larger pore size particles are selected. Why choose BEH SEC column technology?

• Less ionic interactions with stable Diol-coating for confidence in recovery and reproducibility
• Unlike silica-based particles, BEH is pH stable >pH 10 for method flexibility
• Outstanding column lifetime
• Scalable chemistries for method transfer
• Pore size options include 125 Å, 200 Å, and 450 Å depending on the protein of interest
• Synthesized batches are QC tested with relevant samples to help ensure consistency

GPC & SEC Columns

Gel permeation chromatography (GPC) is a chromatographic technique that separates molecules based on their relative size in solution. As such, gel permeation and size exclusion chromatography (SEC) are synonymous terms along with gel-filtration chromatography (GFC) and gel chromatography. However, the terms "gel permeation chromatography” or “GPC” are often used by scientists who analyze polymers (plastics or other synthetic polymers) using either organic or aqueous eluents. In some cases, GFC may be used to refer to GPC analysis for aqueous soluble polymers (e.g., polysaccharides, cationic, anionic, and amphoteric polymers). Waters offers GPC/SEC columns for both organic and aqueous polymer separations, and SEC columns for monoclonal antibodies, proteins, peptides, and other biologics. 

Nano- and Microflow LC-MS Columns

Separation and Trap Columns

Waters nano- and microflow LC-MS columns are specifically designed for low-dispersion nano LC systems.

The selected stationary phases fully leverage the separation power of sub-2-µm particle technology. This delivers the efficiency and selectivity required for complex separations such as discovery of low abundance species, peptide mapping, protein identification, and biomarker discovery.

• Leverage the efficiency of sub-2-µm particle technology at high flow rates
• Stationary phases with a variety of characteristics suitable for biomolecule separations
• Easy interface with LC-MS systems

nanoACQUITY UPLC Columns

nanoACQUITY Trap and Separation Columns

nanoACQUITY Columns and trap columns are the previous generation of columns for nano- and microflow LC-MS applications. They are being replaced by the new generation of ACQUITY UPLC M-Class columns:

• Higher pressure rating (15,000 psi/1,034 bar) enable higher flow rates and thus shorter run times and higher sample turnover.
• M-Class Columns are compatible with nanoACQUITY UPLC systems.

Preparative (Purification) HPLC Columns

Bridging the Gap from Analytical to Prep

As a medicinal chemist working on a challenging drug discovery project, or as purification scientist attempting to maximize yield, your background in chromatographic separations has meant that up until recently you have mainly worked with other chemists to successfully isolate compounds. However, the decline in the number of drugs approved for release onto the market in recent years has put pressure on you and your team to refine the drug discovery process or better refine your purification process and use more effective methods to increase throughput.