|Outer dimensions (w x l)||25.5 x 75.5 mm²|
|Number of wells||8|
|Dimensions of wells (w x l x h)||9.4 x 10.7 x 9.3 mm³|
|Volume per well||300 µl|
|Total height with/without lid||10.8/9.5 mm|
|Growth area per well||1.0 cm²|
|Coating area per well||2.2 cm²|
|Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm|
The Coverslip Bottom
The µ-Slide 8 Well high Glass Bottom comes with a high precision D263 M Schott glass, No 1.5H (170 +/– 5 µm) Glass Coverslip Bottom. This Bottom is ideally suitable for high-resolution microscopy and special microscopic applications like TIRF or super-resolution.
The ibidi µ-Slide 8 Well high Glass Bottom allows for standard immunofluorescence protocols to be employed without the use of coverslips in an all-in-one chamber. All steps (e.g., cell cultivation, fixation, staining, and imaging) are carried out in the open well geometry. After staining, the sample can be observed through the coverslip bottom using high-resolution microscopy.
Fluorescence staining of adherent fibroblast cells.
Blue: nuclei (DAPI). Green: F-actin cytoskeleton (Alexa488-Phalloidin), widefield fluorescence, objective lens 40x, oil immersion.
The µ-Slide 8 Well high Glass Bottom enables high-resolution live cell imaging using different brightfield and fluorescence techniques including TIRF and super-resolution microscopy.
Together with the ibidi Stage Top Incubation System, you can keep your cells happy for a long time by precisely controlling temperature, humidity, and CO2 concentration on your microscope.
Live Cell Imaging of rat fibroblasts in the µ-Slide 8 Well high Glass Bottom.
The ibidi Stage Top Incubation System was used to keep the cells at 37°C and supply 5% CO2. Phase contrast and widefield fluorescence imaging at 3 min intervals, 60x objective lens, oil immersion. Nuclei in blue (NucBlue™ Live ReadyProbes™ Hoechst 33342).