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Surface Modification: #1.5 (170µm + 20/-10µm) D 263 M Schott glass sterilized.

Product outline:

  • A chambered coverslip with 8 individual wells, high walls, and a #1.5H glass coverslip bottom, suitable for use in TIRF and single molecule applications
  • A cell culture chamber for observation of the sample through a glass coverslip bottom using high resolution microscopy
  • Brilliant cell imaging thanks to the low thickness variability of the coverslips’ glass
  • Cost-effective experiments using small numbers of cells and low volumes of reagents
  • Extra high individual walls to keep cross contamination between wells as low as possible

Applications

  • Cultivation and high-resolution microscopy of cells
  • TIRF and single molecule applications of living and fixed cells
  • Super-resolution microscopy
  • Immunofluorescence staining and fluorescence microscopy of living and fixed cells
  • Live cell imaging over extended time periods
  • Transfection assays
  • Differential interference contrast (DIC) microscopy when used with a DIC lid

Technical specifications

Outer dimensions (w x l) 25.5 x 75.5 mm²
Number of wells 8
Dimensions of wells (w x l x h) 9.4 x 10.7 x 9.3 mm³
Volume per well 300 µl
Total height with/without lid 10.8/9.5 mm
Growth area per well 1.0 cm²
Coating area per well 2.2 cm²
Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Technical features

  • Chambered coverslip with 8 independent wells and a non-removable glass coverslip-bottom
  • Bottom made from D 263 M Schott glass, No. 1.5H (170 +/- 5 µm)
  • May require coating to promote cell attachment
  • Individual well walls for minimizing well-to-well crosstalk and contaminations
  • Compatible with staining and fixation solutions
  • Also available as a µ-Slide 8 Well high with an ibidi Polymer Coverslip Bottom for superior cell growth
  • Also available as an adhesive version without a bottom: sticky-Slide 8 Well high
  • Additional version available with a 500 µm grid: µ-Slide 8 Well high Grid-500

The Principle of the µ-Slide 8 Well high

  • The Coverslip Bottom

    The µ-Slide 8 Well high Glass Bottom comes with a high precision D263 M Schott glass, No 1.5H (170 +/– 5 µm) Glass Coverslip Bottom. This Bottom is ideally suitable for high-resolution microscopy and special microscopic applications like TIRF or super-resolution.

Application examples

Immunofluorescence

The ibidi µ-Slide 8 Well high Glass Bottom allows for standard immunofluorescence protocols to be employed without the use of coverslips in an all-in-one chamber. All steps (e.g., cell cultivation, fixation, staining, and imaging) are carried out in the open well geometry. After staining, the sample can be observed through the coverslip bottom using high-resolution microscopy.

Fluorescence staining of adherent fibroblast cells.
Blue: nuclei (DAPI). Green: F-actin cytoskeleton (Alexa488-Phalloidin), widefield fluorescence, objective lens 40x, oil immersion.

Live cell imaging

The µ-Slide 8 Well high Glass Bottom enables high-resolution live cell imaging using different brightfield and fluorescence techniques including TIRF and super-resolution microscopy.

Together with the ibidi Stage Top Incubation System, you can keep your cells happy for a long time by precisely controlling temperature, humidity, and CO2 concentration on your microscope.

Live Cell Imaging of rat fibroblasts in the µ-Slide 8 Well high Glass Bottom.
The ibidi Stage Top Incubation System was used to keep the cells at 37°C and supply 5% CO2. Phase contrast and widefield fluorescence imaging at 3 min intervals, 60x objective lens, oil immersion. Nuclei in blue (NucBlue™ Live ReadyProbes™ Hoechst 33342).

Ibidiibidi is a leading supplier for functional cell-based assays and advanced products for cellular microscopy. ibidi is located in Gräfelfing, Germany, close to Munich, and the US headquarters, ibidi USA Inc., is located in Fitchburg, Wisconsin.
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