Live cell imaging that requires incubator conditions on the microscope, for example:
High-resolution imaging applications that require extra focus stability, for example:
Live cell imaging using the ibidi Stage Top Incubation System shows the gap closure of MCF7 cells in a wound healing assay.
Cells react sensitively to changes in their environment. Factors such as temperature, humidity, and CO2/O2 levels significantly influence the outcome of cell culture assays. It is therefore crucial to precisely maintain the optimal conditions throughout the whole live cell imaging experiment.
The ibidi Stage Top Incubation Systems are available for one slide/dish (Universal Fit) and for multiwell plates (K-Frame). They allow for the accurate and reliable control of temperature, CO2 and O2 concentration, and relative humidity during short-term and long-term live cell imaging assays on any inverted microscope.
(Figure: With fast and precise regulation of important environmental parameters, the ibidi Stage Top Incubators provide stable conditions for live cell imaging directly on the microscope.)
The Heated Lid of the ibidi Heating System reliably prevents condensation, which can reduce the imaging quality during live cell microscopy.
By heating the lid to a temperature higher than the plate, a vertical temperature gradient is created. This gradient, plus the humidity control, prevent lid condensation of the cell culture dish. The required temperature of the cell environment is constantly maintained.