ATTO - Luminometer

Available models

WSL-2300 Phelios AL - ABS/LUMI/NANO Multimode Microplate reader

Technical Specifications
Measurement type	Absorbance (ABS)/Luminescence
Measurement method	Absorbance measurement: Photodiode Luminescence measurement: PMT
Measurement mode	Absorbance measurement: ① Endpoint ② Kinetics ③ Spectrum ④ Well-area scan Luminescence measurement: ① Endpoint ② Kinetics Microvolume measurement: ① Endpoint ③ Spectrum
Detection sensitivity	Absorbance measurement: 0 - 4.0 O.D. Luminescence measurement: 10-18 mole ATP
Measurement plate (Micro plate)	6 / 12 / 24 / 48 / 96 / 384 well plate
Measurement plate（Nano Volume Plate)	3 × 8（24 samples）
Size / Weight	335mm（W）× 305mm（D）× 232mm（H）/ 7.0kg（AC adapter 0.5kg）
	View the entire technical specifications in the brochure.

Multimode microplate reader for multi-purpose

A multimode microplate reader measuring absorbance (ABS), kinetics, spectrum, area scan and luminescence (LUMI), with a wavelength range of 200 to 999 nm. It is also capable of measuring microvolume samples (NANO: 2L).

It is a multi-plate format compatible with 6/12/24/48/96/384 well plates. The "quantitative analysis mode" of quantitative analysis automatically establishes calibration curves and converts concentrations during the measurement, and the analysis results can be saved in Excel.

"Qualitative mode" enables users to establish cutoff values and determine whether target molecules are positive or negative. Furthermore, the optional "Nano Volume plate" can be used to measure absorbance and spectral properties of 24 samples in a minimum volume of 2 µL.

Visit the brochure to find out more about the application.

AB-2350 Phelios - Luminometer for 96/384 well plate

Technical Specifications
Sample format	96/384 well plate format
Detector	Photomultiplier (PMT) Photon counting methods
Spectral range	350～670 nm
Filters	F0: no filter, F1: 560nm LP, F2: 600nm LP
Color separation	Up to three luminescence colors can be separated with automatic filter change mechanism
Injectors	Built-in plunger type 96 well: 20 to 250 uL 384 well: 20 to 100 uL Additional pump system (optional, available for only 96 well plate)
Temperature control	Ambient＋5 ºC～40ºC
Counting times	96 well 1 to 1200 sec, 384 well 1 to 600 sec
Size	398 (W) × 460 (D) × 286 (H) mm/16.5 kg
Power	100-240V 50/60Hz 100VA
	View the entire technical specifications in the brochure.

Phelios(AB-2350) is a microplate luminometer for measuring multiple samples with high efficiency of light detection. Phelios is available to measure the diverse wavelength of bioluminescence from 350 to 670nm in 96,384 well microplate. It is designed to achieve high sensitivity with an optical system that is extremely desirable for bioluminescent detection in low noise photon counting.

Additionally, it is capable of reproducible measurement by means of an internal temperature controller and an accurate pump in repeated injection. It is also possible to measure the samples by automatic filtering even when different multi-colors are generated.

Key Features

Wide wavelength range detection between 350-670 nm - Red luminescence can be detected with high sensitivity
Wide dynamic range (8 logs)
High sensitivity - Detection limit is 1zmole (10-21 moles) of luciferase molecules.
Temperature control system (Ambient+5ºC～40ºC) - Offer appropriate condition to measure light from living cells and tissue samples.
Auto injection system - Programmable injector condition which enable to detect flash-based luminescence
Color separation system - Tricolor of luminescence can be separated and analyzed automatically with filter system (560LP, 600LP)
Measuring samples from flash-based light (20 msec) to long period observation (7 days)

Visit the brochure to find out more about the application.

Method	Mode		Plate type
Absorbance measurement(ABS)	Endpoint	1-6 wavelengths can be set. The absorbance of a selected well is measured once at each wavelength. The measured value is displayed as Optical Density (O.D.).	384/96/48/24/12/6
	Kinetics	Measured over time, the readings for each time are displayed. The measurement is repeated at set intervals until it's reached to the set measurement time.	384/96/48/24/12/6
	Spectrum	Measure the absorbance spectrum in a set wavelength range of 200 - 999 nm and wavelength interval (2 nm<).	384/96/48/24/12/6
	Area scan	Set the scan pattern (3 x 3 to 13 x 13 area) in each well of the microplate and measure the absorbance for each well area	96/48/24/12/6
Luminescent measurement (LUMI)	Endpoint	Measures a luminescence of a specifi ed well once in a set measurement time (10-1000ms). It is displayed in relative luminescence units (RLU).	384/96/48/24/12/6
	Kinetics	Measured over time, the readings for each time are displayed. The measurement is repeated at set intervals until it's reached to the set measurement time.	384/96/48/24/12/6
Micro-volume measurement (NANO)	End point	Use 2 - 5μL of nucleic acid or protein as a sample, and measure the wavelengths 260 nm and 280 nm. The 260/280nm ratio and sample concentration are converted from the measured values.	Nano Volume Plate
	Spectrum	Use 2 - 5 µL of nucleic acid or protein as a sample, and measure the spectrum in a wavelength of 240 - 320 nm (2 nm interval).	Nano Volume Plate
	*To use Micro-volume measurement (NANO), 'Nano Volume Plate' is required as an option.

Method

Mode

Plate type

Absorbance measurement(ABS)

Endpoint

1-6 wavelengths can be set. The absorbance of a selected well is measured once at each wavelength. The measured value is displayed as Optical Density (O.D.).

384/96/48/24/12/6

Kinetics

Measured over time, the readings for each time are displayed. The measurement is repeated at set intervals until it's reached to the set measurement time.

384/96/48/24/12/6

Spectrum

Measure the absorbance spectrum in a set wavelength range of 200 - 999 nm and wavelength interval (2 nm<).

384/96/48/24/12/6

Area scan

Set the scan pattern (3 x 3 to 13 x 13 area) in each well of the microplate and
measure the absorbance for each well area

96/48/24/12/6

Luminescent measurement (LUMI)

Endpoint

Measures a luminescence of a specifi ed well once in a set measurement time (10-1000ms). It is displayed in relative luminescence units (RLU).

384/96/48/24/12/6

Kinetics

Measured over time, the readings for each time are displayed. The measurement is repeated at set intervals until it's reached to the set measurement time.

384/96/48/24/12/6

Micro-volume measurement (NANO)

End point

Use 2 - 5μL of nucleic acid or protein as a sample, and measure the wavelengths 260 nm and 280 nm. The 260/280nm ratio and sample concentration are converted from the measured values.

Nano Volume Plate

Spectrum

Use 2 - 5 µL of nucleic acid or protein as a sample, and measure the spectrum in a wavelength of 240 - 320 nm (2 nm interval).

Nano Volume Plate

*To use Micro-volume measurement (NANO), 'Nano Volume Plate' is required as an option.

WSL-2300 Phelios AL Offers Simple and Easy ABS Operation

WSL-2300 Phelios AL is a multimode microplate reader for measuring absorbance (ABS), luminescence (LUMI), and microvolume samples (NANO: 2µL). Phelios AL enables the use of a wide range of applications and is also superior in terms of performance and economy.