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View details of the full range of BTX Electroporation systems

Available models

Display Type
16-character liquid crystal LCD backlit
Power Source Voltage
100 to 240 VAC, 50 to 60 Hz, CAT I
Operating Temperature
10°C to 40°C
Cooling
Convection through metal case
Relative Humidity
60%
Pollution Degree
Not to be operated in conductive pollutants atmosphere
Altitude
2,000 m (operating)

Economical electroporation system for gram negative bacteria and yeast applications

ECM 399 Generator

The ECM 399 is an exponential decay wave electroporaton generator specifically designed to deliver the field strengths and pulse lengths required for the simple transformation of bacteria and yeast cells. In low voltage mode the ECM 399 has a limited capability for transfecting some mammalian cell lines.

The ECM 399 is ideal for basic transformation in research and academic environments.

The ECM 399 is intended For Research Use Only.  Not for use in diagnostic, pre-clinical, or clinical procedures.

Key features

  • Highest transformation efficiencies of basic bacteria and yeast strains
  • Easy to operate
  • Cost effective
  • Compact in size and portable
  • Available as complete system with cuvettes and cuvette rack
User Interface
Touch Screen Display, Footswitch
Voltage Range
50 to 1,000 Volts
Pulse Width Range
0.050 to 10 ms
Pulse Interval
0.200 to 1,000 ms (5 kHz to 1 Hz)
Pulse Amplitude
50 to 1,200 Volts
Data Export
USB Flash Drive
Dimensions (W x H x D)
32 cm x 20 cm x 40 cm
(12.6 x 7.9 x 15.7 in)
(12.6 x 7.9 x 15.7 in)
Weight
25 lb (11.3 kg)

Advanced electroporation solution for fast, efficient transfection of up to 10 mL of cell suspension

The AgilePulse MAX System is an advanced electroporation solution for fast, efficient transfection of up to 10 ml of cell suspension. Specifically engineered for large-volume applications, our system maximizes cellular uptake with minimal heating and short cycle-time to ensure high cell viability in further cell processing.

The AgilePulse MAX System is simple to use. Cells and polynucleotide are suspended in our proprietary BTXpress Cytoporation Medium T or Cytoporation Medium T4 and transferred via sterile syringe to the large volume electroporation chamber where a programmed sequence of electric pulses is applied. First, a sequence of short, high-intensity pulses opens pores in the cell membranes. These are followed by long, low-intensity pulses that drive the material into cells via electrophoresis. Our patented PulseAgile technology optimizes these pulse parameters to maximize efficiency and cell viability.

The system includes a user-friendly, programmable waveform generator with patented PulseAgile technology, our patent-pending large-volume electroporation chamber, and proprietary BTXpress Cytoporation Medium T, optimized for large volume electroporation. The system is engineered to provide uniform electric fields in a stable temperature environment for excellent cell viability.

Key features

  • Scale-up—transfection protocols readily scale-up from standard laboratory cuvettes to large-volume transfection in the AgilePulse MAX system.
  • Maximal Efficiency with Cytoporation Medium—BTXpress Cytoporation Medium T used with the AgilePulse MAX system has been optimized for maximal efficiency with a number of cell lines, including K562, A20, HEK293 and CHO-KI. It is compatible with a large range of transfectants including DNA, RNA, siRNA, and olignonucleotides. It can be directly diluted in complete growth medium for post-electroporation cell culture.
  • Simple User Interface—all controls are operated with the simple touch screen on the front panel. Data is quickly retrieved by USB key and can be analyzed for detailed pulse characteristics including pulse and pulse current.
  • Pulse Agile® Advantage—transfection efficiency and cell viability are enhanced by specialized, programmable electrical pulse waveforms, particularly important for larger polynucleotide delivery such as DNA plasmids. The patented Pulse Agile technology combines a unique sequence of short high-intensity pulses to porate cell membranes, followed by long low-intensity pulses to further drive transfectants into cells via electrophoresis, while maintaining cell viability.
Model
GEMINI X2
Voltage Range: LV Mode
5 to 500 V in 1 V steps
Voltage Range: HV Mode
505 to 3000 V in 5 V steps
Voltage Accuracy
5%
Pulse Length Range: LV Mode
10 μs to 999 μs in 1 μs steps 1 ms to 999 ms in 1 ms steps
Pulse Length Range: HV Mode
10 μs to 600 μs in 1 μs steps

The Gemini Twin Wave Electroporators are flexible systems allowing both square wave and exponential decay wave electroporation in a single unit. Ideal for all of your electroporation needs, including CRISPR, in vivo, in vitro, in ovo and more. Systems for cuvettes or plates.

These waveform combinations enable researchers to easily and efficiently electroporate eukaryotic and prokaryotic cells in cuvettes or plates with one easy-to-use setup. Prokaryotic cells typically respond well to exponential decay wave pulses and eukaryotic cells are transfected most efficiently with square wave pulses. Combining these two waveforms gives researchers total flexibility to achieve the highest efficiency for their applications.

Key features

  • Square wave and exponential decay waves power in a single unit
  • Multiple pulsing capabilities in both square and exponential decay waveforms (X2 systems)
  • Large, easy-to-use touch screen interface
  • Universal electroporation – transfects cells in vitro, in vivo, in ovo and adherent forms
  • Preset protocols – includes the most common eukaryotic and prokaryotic cell types
  • User-defined protocols – unlimited ability to add and modify protocols
  • Safety – displays resistance measurements for each pulse with three layers of arc protection
  • Data management – stores logs of every pulse delivered for QC and troubleshooting
Display Type
7 inch color touchscreen interface
Power Source Voltage
100 to 240 VAC, 50 to 60 Hz, CAT II Power 350 W (Pulsing), 35 W (Idle)
Fusing
Type 3AG, 5 A, 250 V 1/4 x 1 1/4 in; Type T (time delay)
Intended Use
Indoor use only
Operating Temperature
4° C to 40° C
Cooling
Convection through metal case
Relative Humidity
20% to 80% RH, non-condensing

Electroporation system primarily used for bacteria and yeast transformation. High throughput (25- and 96-well) models available.

ECM 630 Generator

The ECM 630 is an exponential decay wave electroporation generator providing a broad range of voltage and time constants for full flexibility in varying transfection applications. The ability to select the resistance and capacitance values, and adjust the range of voltages is the key to achieving the optimal time constants and field strengths needed for efficient transformation of prokaryote and eukaryote transfection.  The ECM 630 can perform the widest range of electroporation applications among commercially available electroporation generators.

Flexibility is important, so BTX has designed a plug and play system for our ECM 630 system to transition between standard cuvettes and to 25/96-well plates using High Throughput Plate Handlers.

Legacy ECM 630 Systems are non RoHS compliant, and thus not available for purchase in the European Union. While supplies last, Legacy ECM 630 systems are available to customers in the Americas and Asia as we transition to new ECM 630 instruments.

The ECM 630 is intended For Research Use Only.  Not for use in diagnostic, pre-clinical, or clinical procedures.

Key features: New ECM 630

  • Large, easy to use color touchscreen interface
  • Preset protocols for transforming the most common bacteria, yeast, and microorganisms
  • High-Throughput Plate Handler setting for automatic processing of up to 96 samples at a time with an HT-200 Plate Handler
  • Create, modify, and store up to 1,000 user defined protocols
  • Enhanced Safety Features: Pre-pulse sample resistance check, arc protection, and over-current pulse abort
  • Data Management: Experimental data log stores parameters of all pulses delivered and every sample resistance for QC and troubleshooting purposes
  • Voltage range of 5 V to 3,000
  • Capacitance range of 25 µF to 3,275 µF in 25 µF steps in LV mode; 10, 25, 35, 50, 60, 75, 85 µF in HV mode.
  • Resistance range of 25 ohm to 1,575 ohm in 25 ohm steps.

Key features: LEGACY ECM 630

  • Digital user interface; push button rotary knob to set parameters
  • Voltage range of 10 V to 2,500 V
  • Capacitance Range of 25 µF to 3,275 µF in 25 µF steps
  • Resistance range of 25 ohm to 1,525 ohm in 25 ohm steps. The additional resistor selection "None" allows researchers to reproduce protocols from competitive systems
Display Type
7 inch color touchscreen
Power Source Voltage
100 to 240 VAC, 50 to 60 Hz, CAT II Power 500 W (Pulsed), 50 W (Idle)
Fusing
Type 3AG, 5 A, 250 V; Type T (time delay)
Intended Use
Indoor use only
Operating Temperature
10°C to 40°C
Cooling
Convection through metal case
Relative Humidity
60%
Altitude
2,000 m (operating)

The ECM 830 is a versatile electroporation system suitable for applications for gene, drug and protein delivery including: CRISPR, mammalian cells, in vivo, ex vivo, in ovo tissue, ex plant and nuclear transfer. 

ECM 830 Generator

The ECM 830 is a square wave pulse generator designed for in vitro and in vivo applications. The versatility of the ECM 830 applications for gene, drug and protein delivery include; mammalian cells, in vivo, ex vivo tissue, zebra fish tissue and embryos, nuclear transfer, embryo manipulation, plant protoplast and basic bacteria and yeast transformations.

The ECM 830 can be used in combination with a wide array of BTX specialty electrodes and accessories to enhance your molecular and drug delivery experiments.

The ECM 830 is intended For Research Use Only.  Not for use in diagnostic, pre-clinical, or clinical procedures.

Key features

  • A wide range of voltages from 5 to 3,000 V
  • Pulse durations from 10 μsec to 999 ms
  • Pre-Pulse Resistance Check, Over-Current Pulse Abort, and Instrument Arc Protection Safety Features
  • Easy to use touchscreen user interface
  • Logs of every pulse delivered for QC and troubleshooting
Frequency
1 MHz
Voltage
0 - 75 Vrms
Duration
0 - 99 sec
Post fusion AC
1/10 of pre-fusion amplitude
Post fusion ramp
1 - 9 sec
Pause between AC/DC
50 µsec

The Legacy ECM 2001 multi-purpose electro cell manipulation generator produces a proprietary AC waveform for electrophoretic alignment of cells and a square DC waveform for cell fusion.

LEGACY ECM 2001 Generator

The LEGACY ECM 2001 is a multipurpose electro cell manipulation pulse generator capable of performing both electrofusion for hybridoma production, hybrid cell formation and nuclear transfer applications in addition to electroporation of cells in suspension and in vivo applications. This versatile platform combines microprocessor-controlled precision with a wide range of possible parameters for a wide range of applications when combined with standard cuvettes, high throughput plates, specialty electrodes and other available accessories.

The Legacy ECM 2001 is intended For Research Use Only.  Not for use in diagnostic, pre-clinical, or clinical procedures.

Key features

  • Wide range of voltages from 5 to 3000 V
  • Finer voltage discrimination
  • Pulse durations from 10 µsec to 10 sec
  • Arc quenching
  • Digital display of actual voltage and pulse length delivered
User Interface
Touch Screen Display, Footswitch
Voltage Range
50 to 1,000 volts
Pulse Width Range
0.050 to 10 ms
Pulse Interval
0.200 to 1,000 ms (5 kHz to 1 Hz)
Data Export
USB Flash Drive
Dimensions (W x H x D)
Dimensions (W x H x D)
(with handle)
32 cm x 20 cm x 40 cm
(12.6 in x 7.9 in x 15.7 in)
(12.6 in x 7.9 in x 15.7 in)
Weight
25 lb (11.3 kg)
Operating Temperature
10 to 40oC
Mains Voltage
100 to 250 VAC
Fuse
5 Amp Slo-Blo, 5 mm x 20 mm
Software
IM (Intra-muscular) or ID (Intra-dermal)

The AgilePulse In Vivo System, used for animal immunization, vaccine development, and gene therapy, provides an intra-muscular, intra-dermal, or intra-tumor electroporation solution to produce maximum transfection efficiency.

Traditional in vivo nucleic acid delivery systems, such as gene gun delivery, suffer from poor efficiency. The AgilePulse In Vivo System effectively introduces DNA or RNA into the target tissue, representing a powerful and safe means for stimulating an immune response that recognizes and eliminates target molecules in the body.

For vaccine applications, DNA vaccination through the dermal layer is preferred since it is an easily accessible site that is immunologically active. After direct injection of plasmid DNA in the dermal layer, a programmed sequence of electric pulses is applied through a miniature parallel needle electrode array to promote cellular uptake and transfection. Cells in the surrounding tissue are transfected, including dendritic antigen-presenting cells and mesenchymal origin cells. Gene expression simulates the immune system to respond to the secreted antigen. Gene expression in skin is 100-fold higher when delivery is enhanced by electroporation compared to simply injecting plasmid DNA (Roos, et. al. 2009).

Key features

  • Simple User Interface—touch screen user interface, USB key data storage, and Windows® Mobile 6.0 operating system. The system automatically stores a digitized record of all pulse parameters for quality control. EN 60601-1, EN 60601-1-1 EN 60601-1-2, UL60601-1 and CSA601.1 compliant.
  • Pulse Agile® Advantage—patented Pulse Agile technology combines a unique sequence of short, high-intensity pulses to open pores in cell membranes, followed by long, low-intensity pulses to further drive the DNA into cells via electrophoresis. The adjustable pulses improve efficiency while maintaining cell viability. The specialized use of the Pulse Agile waveform has shown to significantly enhance antigen specific CD8+ T-cell response when standard protocols do not.
  • Uniform, Reliable Electroporation—AgilePulse parallel-needle arrays produce uniform electric fields across the treatment area for a more thorough transfection of the tissue. Electrode impedance is monitored through software analysis to ensure reliable electrode placement in tissue each and every time.
  • Simple, Fast Treatment—simple yet effective intra-dermal or intramuscular electrode design allows for shorter delivery times of less than a second. The electrodes provide a uniform pulse to cover and target larger areas of tissue using a single pulsing application.
  • Optimal Design—miniature, 2-mm length needles easily penetrate the layers of skin or muscle and target cells for high efficiency gene delivery.
  • Safety—Each needle electrode array comes with a safety cover and easy grip sides to make the electrode insertion procedure simple and safe.
Voltage Range: LV Mode
5 to 500 V in 1 V steps
Voltage Range: HV Mode
505 to 3000 V in 1 V steps
Voltage Accuracy
5%
Pulse Length Range: LV Mode
10 to 999 µs in 1 µs steps
Pulse Length Range: HV Mode
10 to 600 µs in 1 µs steps

The ECM 2001+ Electrofusion and Electroporation System provides fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications, as well as square wave electroporation for transfecting genes and other molecules into mammalian cell lines.

ECM 2001 Generator

The ECM 2001+ is a multifunctional electrofusion and square wave electroporation generator. The ability to generate both AC and DC waves allows for fast and efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer applications. This system is powerful enough to yield high transfection efficiencies for cell lines and difficult to transfect cell types including stem cells and primary cells. The gentle square wave pulse also allows for high cell viability of these cell types.

The ECM 2001+ is intended For Research Use Only. Not for use in diagnostic, pre-clinical, or clinical procedures.

Waveforms

AC sine wave aligns cells by dielectrophoresis for electrofusion applications. Square DC waveform provides the fusion pulse for electrofusion or is utilized in mammalian electroporation applications.

Key features

  • AC waveform of 0.2 – 2.0 MHz
  • Square wave electroporation capabilities
  • A wide range of voltages from 5 V to 3000 V
  • Fine voltage discrimination
  • Advanced programming capability to combine multiple AC steps pre- and post-DC fusion step
  • Capable of operating at low impedance loads
  • Large touchscreen interface
BTX

BTX has been at the forefront of electroporation technology since we introduced the first commercially available electroporator in 1983. For over 30 years, we have made it our priority to focus only on electroporation and electrofusion. This focus has allowed us to develop the experience and expertise to supply you with a broad selection of innovative in vivo and in vitro tools to advance your research.

Whatever your need, our range of electroporation systems and accessories covers virtually every electroporation and electrofusion application, including high throughput. Additionally BTX provides exceptional applications support and service before, during and after your purchase to ensure your success.

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