Isothermal titration calorimetry (ITC) is a high-resolution method for complete characterization of the basic chemical details of a binding interaction. The method determines a binding constant, reaction stoichiometry, and total reaction energy of two or more compounds (small or large molecules) in solution. Isothermal titration calorimeters do this by measuring the heat that is released or absorbed when molecules interact with each other. Temperature conditions can be controlled to understand the temperature dependence of binding.
Affinity ITC
Standard Volume | Low Volume | |
---|---|---|
Minimum Detectable Heat | 0.1 µJ | 0.04 µJ /0.05 µJ |
Maximum Measurable Heat | 5,000 µJ | 5,000 µJ |
Low Noise Level | 0.0025 µWatt | 0.0013 μW/ 0.0014 µWatt |
Baseline Stability | 0.02 µWatt/hr | 0.02 µWatt/hr |
Nano ITC
Standard Volume | Low Volume | |
---|---|---|
Sample Cell Size | 1.0 mL | 190 µL |
Injection Syringe Volume | 100 µL & 250 µL | 50 µL |
Cell Geometry | Fixed Cylindrical | Fixed Cylindrical |
Cell Composition | 24K Gold / Hastelloy | 24K Gold |
The Affinity ITC and ITC Auto are designed for the most challenging life science laboratory environments that require high sensitivity, high productivity and the most advanced ITC technologies. The Affinity ITC, along with the Nano DSC, are used in gene therapy applications for characterizing serotype and drug loading in AAVs and LNPs, as well as target and off-target binding interactions. The Affinity ITC brings advanced engineering to all critical aspects of the measurement ensuring the highest quality ITC data.
Key Features
The Nano ITC Standard Volume and Nano ITC Low Volume isothermal titration calorimeters are designed to provide maximum sensitivity and flexibility for the study of biomolecular binding. Both instruments use solid state thermoelectric heating and cooling systems to precisely control temperature, and have the same flexible injection syringe assemblies for efficient and accurate delivery of titrant. The true isothermal power compensation design of the Nano ITC instruments provides the highest sensitivity and flexibility for an ultrasensitive ITC analyzing biological samples in-solution.
Key Features