Exploring Biological Buffers and Their Uses in the Pharmaceutical Industry

In the past, different inorganic substances, such as phosphate, cacodylate, borate, and bicarbonate, were used as buffers, with organic acids being added to the list to conduct biological experiments. However, the disadvantage of these buffers was that they were not inert, because these substances were enzymes substrates or enzyme inhibitors and could react with metabolites or other components. For a buffer to be effective it should not be subject to either enzymatic or non-enzymatic changes and therefore be inert.

The right buffering system can protect the integrity of biomolecules and reagents and provide solution stability and pH control without interfering with biological processes. Buffer systems also need to supply critical salts and nutrients for cells and tissues to thrive. The right system can bring stability to cell culture, polymerase chain reaction (PCR), drug screening, bioprocessing, purification, and final formulation applications.

Essential properties of an effective buffer:

• Solubility
• Permeability through biological membranes is an important requirement
• pKa value at the mid-point of the range of the test system
• Change in pKa value should be dependent on temperature
• Change in pKa value should also be dependent on dilution
• Interaction with other components (e.g. metal ions, enzymes)
• UV absorption
• Non-toxic

The concentration of your buffer must be sufficient to account for the amount of acid or base you plan to use in your experiment. The more the pH-altering component you plan to use, the higher the buffer concentration you will need.

Buffer Toxicity

Buffer toxicity is highly important because some buffers can be toxic to the cells you are working with. If unsure about the toxicity, you can test the buffer on the cells before using it in the experiment.

Temperature

As temperature can alter the buffering capacity of your buffer, checking the temperature for your experiment is essential.

Reactivity

Finally, ensuring that your chosen buffer does not produce unwanted reactions in your experiment is important to conduct a reactivity test.

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