Amino acid analysis is a technique based on ion exchange liquid chromatography, used in a wide range of application areas to provide qualitative and quantitative compositional analysis. It is a key part of the ICH Q6B guidelines for characterization of biopharmaceuticals.
Detection System | Nninhydrin - post column derivatization |
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Reproducibility | Area: Better than 0.5% RSD at 10 nanomoles. Retention time: Better than 0.1%RSD |
Pumps | Two dual piston pumps with integrated pressure transducer and drain valve. Ceramic pump head, sapphire pistons and valves. Electronically controlled piston wash. |
Operating Pressures | Buffer pressure: maximum 150 bar. Ninhydrin pressure: maximum 24 bar |
Analytical Column | High pressure PEEK column packed with Ultropac 8 cation exchange resin. Peltier heating/cooling system. |
Sample Injection | 3 injection modes (full loop, partial loop and micro), 84 position autosampler. Sample volumes from 1μL to 5000μL. 200μL loop supplied as standard. |
Temperatures | Column temperature variable between 20°C and 99°C. Reaction Coil temperature adjustable between 40°C and 145°C (135°C is optimum). |
Software | BioSys v3.0 control software Biochrom Alias Manager autosampler control software Latest version of EZChrom Elite Data Handling 21 CFR part 11 compliant |
Photometer | Single flow cell with optical beam splitter with detection at 440nm and 570nm |
Weight and dimensions | Bench top Fluidics Cabinet: 50kg/110lbs, 48 x 59 x 57cm /19 x 23 x 22 inches Autosampler: 21kg/46lbs, 30 x 57.5 x 36cm /12 x 23 x 14in (cooled version) 19kg/42lbs, 30 x 51 x 36cm/12 x 20 x 14in (ambient version) |
Required Services | Oxygen free Nitrogen gas (99.99%) or Argon gas regulated to 5 bar. Drainage facility. 240V/100V, 50Hz/60Hz, 300VA mains supply. |
Safety Systems | Automatic shut-down and reaction coil flushing in the event of: Power failure Photometer lamp failure Low ninhydrin, buffer, coil and nitrogen pressures High coil and column temperatures, high ninhydrin, buffer and coil pressures. |
System
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Use
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Minutes (to Arginine)
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Biochrom 30+ Physiological Accelerated System
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When speed is a decisive factor, with little compromise on the peak resolution
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90min
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Biochrom 30+ Physiological
High Performance System
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The best compromise between speed, resolution and column life
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125min
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Biochrom 30+ Physiological
High Resolution System
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When 100% baseline separation and a maximum number of amino acid separated are required. Ideal for Research and low sample throughputs
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150min
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Biochrom launches the Biochrom 30+ Series of amino acid analyzers - the gold standard in dedicated amino acid analysis. Clinical laboratories worldwide trust this well documented classical amino acid analysis method based on ion exchange chromatography with post column derivatization with Ninhydrin.This allows both qualitative and quantitative analysis of up to 56 amino acids and derivatives, including Argininosuccinic acid, Alloisoleucine and Sulfocysteine.
The Biochrom 30+ Physiological system uses a combination of 5 lithium citrate buffers to achieve a full amino acid profile in less than 90 minutes using the new Accelerated method."
The Biochrom 31+ offers full automation for the fast and reliable quality/authenticity analysis of generic drugs and infusion fluids to a high standard that satisfies legislation. Long-life columns can tolerate various matrices and a high-salt content in the analysis of infusion liquids and recombinant or synthetic peptides. The ability of Biochrom 31+ to rapidly deal with complex samples, requiring virtually no sample preparation makes it a powerful quality-control technique for the analysis of soft drinks and beverages.
Protein hydrolysates are analysed using a sodium citrate-based buffer system. Protein hydrolysates are referred to as samples containing most or all of the 20 amino acids normally found in proteins. A buffer system using 3 or 4 sodium citrate buffers is sufficient for these samples.
This system is designed for the analysis of complex oxidised hydrolysates such as those obtained from food and feedstuffs.
Animal feedstuff, pet food, and the human food industry.
Dieticians need to know the nutritional value of food and amino acid analysis is essential for ensuring nutritional quality. It is the most direct and precise measurement of total protein in food and is a valuable consumer sales aid.
The complex matrix of the products puts high demands on the analysis method e.g. high salt concentration. Buffer systems and columns used in the Bio30+ systems can tolerate those high concentrations.
Limiting amino acids such as lysine, threonine, cystine and methionine may be rapidly identified to enable adjustments to the formulation of feedstuffs.
This system meets the requirements of the standard methods from AOAC and the EU Commission Directive 98/64/EC.
Superb separation, accurately identifies and quantifies free amino acids, as well as the amino acid composition of proteins and peptides.
Minimal matrix interference and near baseline separation helps to reduce ambiguities in interpretation and increase confidence in diagnosis of inborn errors of metabolism. Automated derivatization of amino acids maximizes reproducibility.
Rapid protocols that increase sample throughput by cutting analysis time.
The column cleaning and repacking service, gives columns an almost indefinite life and cuts the cost of ownership.
Total PC control allows 24/7 operations.
Wide variety of sample types compatible: Urine, plasma, serum, CSF, amniotic fluid, culture media, tissue extracts, protein hydrolysates from various food and feedstuff samples or synthetic drugs, nutraceuticals, beverages.
No lengthy method development required as this well characterized system is established worldwide. All customers have their instrument optimised to their application directly from the factory.
Interchangeable sample trays allow samples to be prepared in advance and stored refrigerated.
Advanced fluidics and robust hardware mean easy maintenance for users, backed by the reassurance of Biochrom's dedicated service and support team.
Integrated software, choice of different methods to adapt to the user's needs. Automatic sample set-up. Networking and exporting of data.
Bench top design requiring minimum lab space.