Agilent - HPLC Columns - Zorbax

Agilent ZORBAX Eclipse Plus columns provide the ultimate in performance for silica-based columns.

Peak shape is excellent for the most challenging basic compounds, improving efficiency and resolution with these sample types. These results are achieved by improvements in the silica manufacturing and bonding technology, which is completely controlled by Agilent.

Because of their high level of performance, Eclipse Plus columns are the ideal first choice for method development of all samples. If you need to achieve fast method development and superior productivity, then choose a column with high-resolution 1.8 μm particles. For standard methods, conventional 5 μm and Rapid Resolution 3.5 μm columns are your best choice. With all particle sizes, easy method transfer is possible. With more rigorous QA and QC testing, column lot-to-lot reproducibility is also improved, resulting in long-term reliable results for all analyses.

High resolution separation of 16 PAHs in EPA Method 610

• Extensive range of particle sizes (1.8, 3.5 and 5 μm) and sizes for fast and high resolution separations
• Each batch of material is specifically tested with PAHs for maximum reproducibility under expected operating conditions
• Excellent performance using the high quality, improved silica of Eclipse Plus columns
• Good for applications requiring "shape selectivity" or the separation of geometric isomers

Agilent ZORBAX Eclipse PAH columns are recommended for the separation of polycyclic aromatic

hydrocarbons. PAHs are considered priority pollutants and the analysis of these potentially carcinogenic

compounds in water, soil and food is of major importance. Eclipse PAH columns separate all 16 PAHS in

EPA method 610 quickly and with high resolution.

Four selectivity choices for method development optimization

• Good peak shape for basic, acidic and neutral compounds

• High performance over a wide pH range – pH 2-9

• Particle sizes from 1.8 to 7 μm

• Long lifetime with eXtra Dense Bonding and double endcapping

Agilent ZORBAX Eclipse XDB columns – C18, C8, Phenyl and CN – provide four bonded phase choices for method development optimization. These columns provide good peak shape over a wide pH range (2-9) for additional method development flexibility with one family of columns. Eclipse XDB columns can be used for method development at low pH (2-3) and the same column can be used for method development in the mid pH (6-8) region. In the mid pH region residual silanols are more active and tailing interactions are more likely. To overcome these interactions, Eclipse XDB columns are eXtra Densely Bonded and double endcapped through a proprietary process to cover as many active silanols as possible. The result is superior peak shape of basic compounds from pH 2-9. Eclipse XDB columns are available in 1.8, 3.5, 5 and 7 μm particle sizes for high speed, high resolution, analytical and prep scale separations.

• Longest column lifetime and best reproducibility for low pH separations – down to pH 1
• Patented stable column chemistry allows use at high temperature and low pH without degradation
• Six different bonded phases provide broad selectivity – SB-C18, SB-C8, SB-CN, SB-Phenyl, SB-C3, and SB-Aq
• High purity (Type B) silica for good peak shape

Agilent ZORBAX StableBond columns use patented, unique, nonfunctional silanes with bulky diisobutyl (SB-C18) or diisopropyl (SB-C8, SB-C3, SB-Phenyl, SB-CN, and SB-Aq) side chain groups that sterically protect the key siloxane bond to the silica surface from hydrolytic attack at low pH. StableBond packing materials are not endcapped in order to provide exceptional stability and to maximize lifetime and reproducibility under acidic mobile phase conditions. The high purity, low acidity silica provides excellent peak shape with acidic, basic and neutral compounds making StableBond columns an excellent choice for low pH method development. ZORBAX StableBond columns are compatible with all common mobile phases, including very high aqueous mobile phases.

• Recommended for alternate selectivity at low pH relative to Eclipse Plus C18, Eclipse XDB-C18 and
• StableBond SB-C18; for higher temperature applications, StableBond is recommended
• Higher carbon load than SB-C18 columns (12% vs. 10%)
• High stability and good peak shape for low pH applications (up to pH 8)
• Manufactured using dimethyloctadecylsilane and non-endcapped
• ZORBAX Rx-C8 is the same product as SB-C8

• Excellent peak shape for challenging basic compounds at low and mid pH
• Unique reversed-phase selectivity
• Novel bonding technology with embedded polar group and steric protection
• Usable in 100% aqueous mobile phases

The Agilent ZORBAX Bonus-RP column has a polar amide group embedded in a long alkyl chain. This novel bonding reduces interactions between basic compounds and the silica support, improving peak shape for the most difficult basic compounds. Peak shape and column lifetime are further improved by triple endcapping. In addition, diisopropyl side groups provide steric protection against acid hydrolysis for good lifetime at low pH. The Bonus-RP column provides an alternate selectivity to C18 and C8 alkyl bonded phases.

Agilent Original ZORBAX columns are made with Type A silica and are useful for many applications of acidic or neutral compounds. These columns have a higher activity level and are therefore useful for separating isomers (e.g. cis-trans, geometric) or other compounds where silanol activity enhances selectivity. These columns are used in many established methods.