The Agilent ZORBAX column family is one of the most popular HPLC column families for reversed-phase HPLC. ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution. From ZORBAX Eclipse Plus C18 to sub-2 micron UHPLC columms, a wide selection of stationary phases and particle sizes allows you to fine-tune your selectivity to match your application.
ZORBAX Eclipse Plus columns are the recommended starting point for method development and benefit from the ultimate performance from fully porous silica-based columns.
Agilent ZORBAX Eclipse Plus columns provide the ultimate in performance for silica-based columns.
Peak shape is excellent for the most challenging basic compounds, improving efficiency and resolution with these sample types. These results are achieved by improvements in the silica manufacturing and bonding technology, which is completely controlled by Agilent.
Because of their high level of performance, Eclipse Plus columns are the ideal first choice for method development of all samples. If you need to achieve fast method development and superior productivity, then choose a column with high-resolution 1.8 μm particles. For standard methods, conventional 5 μm and Rapid Resolution 3.5 μm columns are your best choice. With all particle sizes, easy method transfer is possible. With more rigorous QA and QC testing, column lot-to-lot reproducibility is also improved, resulting in long-term reliable results for all analyses.
High resolution separation of 16 PAHs in EPA Method 610
Agilent ZORBAX Eclipse PAH columns are recommended for the separation of polycyclic aromatic
hydrocarbons. PAHs are considered priority pollutants and the analysis of these potentially carcinogenic
compounds in water, soil and food is of major importance. Eclipse PAH columns separate all 16 PAHS in
EPA method 610 quickly and with high resolution.
Four selectivity choices for method development optimization
• Good peak shape for basic, acidic and neutral compounds
• High performance over a wide pH range – pH 2-9
• Particle sizes from 1.8 to 7 μm
• Long lifetime with eXtra Dense Bonding and double endcapping
Agilent ZORBAX Eclipse XDB columns – C18, C8, Phenyl and CN – provide four bonded phase choices for method development optimization. These columns provide good peak shape over a wide pH range (2-9) for additional method development flexibility with one family of columns. Eclipse XDB columns can be used for method development at low pH (2-3) and the same column can be used for method development in the mid pH (6-8) region. In the mid pH region residual silanols are more active and tailing interactions are more likely. To overcome these interactions, Eclipse XDB columns are eXtra Densely Bonded and double endcapped through a proprietary process to cover as many active silanols as possible. The result is superior peak shape of basic compounds from pH 2-9. Eclipse XDB columns are available in 1.8, 3.5, 5 and 7 μm particle sizes for high speed, high resolution, analytical and prep scale separations.
Agilent ZORBAX StableBond columns use patented, unique, nonfunctional silanes with bulky diisobutyl (SB-C18) or diisopropyl (SB-C8, SB-C3, SB-Phenyl, SB-CN, and SB-Aq) side chain groups that sterically protect the key siloxane bond to the silica surface from hydrolytic attack at low pH. StableBond packing materials are not endcapped in order to provide exceptional stability and to maximize lifetime and reproducibility under acidic mobile phase conditions. The high purity, low acidity silica provides excellent peak shape with acidic, basic and neutral compounds making StableBond columns an excellent choice for low pH method development. ZORBAX StableBond columns are compatible with all common mobile phases, including very high aqueous mobile phases.
The Agilent ZORBAX Extend-C18 column uses a novel bidentate C18-C18 bonding technology to make it possible to develop high-resolution separations at high pH with a silica-based column. At high pH, non-charged basic compounds will not interact with the underlying silica. The result is high efficiency separations with superior peak shape and improved resolution. High pH separations are also the best choice for compounds that are more stable or more soluble in high pH solutions.
Some of the mobile phase buffer options for high pH include triethylamine, pyrrolidine, glycine, borate and ammonium hydroxide. Ammonium hydroxide at pH 10.5 is an excellent mobile phase modifier for the LC/MS of peptides and small molecules with improved sensitivity compared with TFA containing mobile phase at low pH. The Extend-C18 column is stable from pH 2-11.5 with good peak shape for all types of compounds. Extend-C18 columns also provide an additional selectivity choice at low pH.
The Agilent ZORBAX Bonus-RP column has a polar amide group embedded in a long alkyl chain. This novel bonding reduces interactions between basic compounds and the silica support, improving peak shape for the most difficult basic compounds. Peak shape and column lifetime are further improved by triple endcapping. In addition, diisopropyl side groups provide steric protection against acid hydrolysis for good lifetime at low pH. The Bonus-RP column provides an alternate selectivity to C18 and C8 alkyl bonded phases.
Agilent Original ZORBAX columns are made with Type A silica and are useful for many applications of acidic or neutral compounds. These columns have a higher activity level and are therefore useful for separating isomers (e.g. cis-trans, geometric) or other compounds where silanol activity enhances selectivity. These columns are used in many established methods.