BMG Labtech - VANTAstar

Detection Modes	Fluorescence intensity (incl. FRET) Fluorescence polarization Luminescence (flash and glow) - incl. BRET Time-resolved fluorescence TR-FRET UV/vis absorbance spectra
Measurement Modes	Top and bottom reading Endpoint and kinetic Sequential multi-excitation Sequential multi-emission Spectral scanning (fluorescence, luminescence, absorbance) Ratiometric measurements Well scanning
Microplate Formats	6 to 1536-well plates, user-definable LVis Plate with 16 low-volume microspots (2 μL)
Microplate Carrier	Robot compatible
Light Sources	High energy xenon flash lamp
Detectors	Low-noise photomultiplier tube CCD spectrometer
Wavelength Selection	Dual Linear Variable Filter (LVF) Monochromators™ Linear Variable Dichroic Mirror Separates ex & em LVF Monochromators Optical filters: Ex and em slides hold up to 5 filters each LVF Monochromators + optical filters Use one for ex and the other for em UV/Vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec/well
Spectral Range	Filters FI, LUM, TRF, FP, TR-FRET: 240 - 740 nm LVF Monochromators™ FI, LUM: 320 - 740 nm Linear Variable Dichroic 340 - 740 nm Spectrometer ABS: 220 - 1000 nm; wavelength precision: ≤ 0.5 nm
Sensitivity	FI filters (top) < 0.5 pM (< 10 amol/well FITC, 384sv, 20 μL). FI filters (bottom) < 2.5 pM (< 125 amol/well FITC, 384g, 50 μL). FI monochromator (top) < 0.8 pM (< 16 amol/well FITC, 384sv, 20 μL). FI monochromator (bottom) < 3.5 pM (< 175 amol/well FITC, 384g, 50 μL). Dynamic Range: 8 decades in a single measurement. FP < 1.2 mP SD at 1 nM FITC (384sv, 20 μL). HTRF^® (black and white microplates) Reader Control Kit (Eu) after 18h (384sv, 20 μL). Delta F > 700% (High Calibrator). Delta F > 25% (Low Calibrator). TRF < 30 fM europium (< 2.4 amol/well, 384, 80 μL). LUM < 0.8 pM (< 15 amol/well ATP, 384sv, 20 μL). Dynamic range: 8 decades in a single measurement. ABS with spectrometer Selectable spectral resolution: 1, 2, 5, and 10 nm. OD range: 0 to 4 OD; photometric resolution: 0.001 OD. Accuracy: < 1% at 2 OD. Precision: < 0.5% at 1 OD and < 0.8% at 2 OD. Linearity: ≤ 0.8% at 2.0 OD.
Read Times	Flying mode (1 flash) 9 sec (96), 16 sec (384)
Shaking	Linear, circular and double-circular with user-definable time and speed
Incubation	+4 °C above ambient up to 45 °C or 60 °C Minimum condensation concept: the upper heating plate of the incubation chamber operates at +0.5 °C than the lower plate, heated optic system
Software	Multi-user Smart Control and MARS Data Analysis Software included FDA 21 CFR Part 11 compliant Integrated fluorophore library
Dimensions	Width: 35 cm, depth: 52 cm, height: 36 cm; weight: 27 kg

VANTAstar Microplate Reader

The VANTAstar is a compact multi-mode microplate reader conceived for ease-of-use and flexibility. It provides the best possible data without the need to make any adjustments, and comes equipped with LVF Monochromators, filter-based detection and a UV/vis spectrometer.