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Live-cell imaging allows researchers to determine not only whether, but also how and when certain biological events occur in cell culture. In order to achieve the high image quality standards required for data analysis and publications, live-cell imaging is performed predominantly using a microscope with a stage top incubation box. However, the culture conditions in the incubation box are more prone to fluctuations, as compared to a dedicated cell culture incubator. Using the CytoSMART Lux3 BR, it is no longer necessary to choose between optimal cell culture conditions and image quality.

Live-cell imaging has become increasingly valuable in the fields of cancer research, drug discovery, immunology, tissue engineering, stem cell research, and 3D cell culture models. Various experimental applications, including cell viability and cell differentiation monitoring, spheroid and organoid characterization, microfluidic chip platforms, cell morphology analysis, single-cell tracking, and angiogenesis monitoring can benefit from detailed kinetic visualization.

Technical Specifications

Channel Brightfield with digital phase contrast
Camera 6.4 MP CMOS
Magnification 10× fixed objective – 20× digital zoom
Image resolution 2072 × 2072 pixels
Field of view 1.45 × 1.45 mm
Light source LED
Data formats JPG, TIFF, XLSX, MP4
Data storage Unlimited cloud storage
Computer requirements Windows 10, USB 3.0
Dimensions (L × W × H) 16.6 cm × 14.0 cm × 13.5 cm
Weight 1.3 kg
Culture vessels Well-plates, petri dishes, flasks, microfluidic chips, and custom culture vessels
Operating conditions 5 – 40°C, 20 – 95% humidity
Warranty 1-year parts and labor
Extra features Confluence analysis algorithm, scratch assay analysis algorithm

Key Features

Incubator-friendly live-cell imaging

The cell culture environment inside a stage top incubation box is generally sub-optimal for maintaining live cells. The CytoSMART Lux3 BR is designed to work inside a standard CO2 incubator, without disturbing the internal airflow and temperature. This allows you to perform long-term cell imaging experiments at the optimum culture conditions.

Digital images suitable for scientific publications

The CytoSMART Lux3 BR is ideal for capturing crisp brightfield images and videos of living cells. The image size of 2072×2072 pixels combined with the 1.45×1.45 mm field of view provide a resolution of 0.7 µm/pixel. Even at the commonly required image resolution of 300 dpi for printed scientific publications, these images can fill the entire page width if desired, without compromising the image quality.

Remote and automated analysis of cell cultures

Images and videos of running or completed experiments can be accessed, processed, and analyzed from any desired location using the CytoSMART Cloud-based environment. Therefore, cells can be monitored without having to open the incubator, or even be in the lab. The integrated cloud-based image analysis facilitates quantification of output parameters, e.g. cell confluence or wound healing rate.

Easy to expand to a multiple-device setup

A setup with the CytoSMART Lux3 BR can be easily expanded to two or even four devices that can be operated and controlled individually via a single laptop. Since all devices of the CytoSMART Lux3 BR Duo or Multi Lux3 BR Kits can be placed directly next to each other in the same incubator, the monitored cultures are maintained in an identical culture environment.

CytoSMART Lux3 BR Duo Kit - Accurate Tracking Of Chemotactic Cell Migration

  • Biopharmaceutical and Biotechnology
CytoSMARTCytoSMART is an innovator in kinetic live-cell imaging. Combining compact and fast imaging hardware with powerful image analysis algorithms supported by cloud computing. Automation in time-lapse microscopy and image based cell counting to generate high-quality and robust data.