|Outer dimensions (w x l)||25.5 x 75.5 mm²|
|Number of wells||8|
|Dimensions of wells (w x l x h)||9.4 x 10.7 x 9.3 mm³|
|Volume per well||300 µl|
|Total height with/without lid||10.8/9.5 mm|
|Growth area per well||1.0 cm²|
|Coating area per well||2.2 cm²|
|Bottom: ibidi Polymer Coverslip|
The Coverslip Bottom
The µ-Slide 8 Well high comes with a thin ibidi Polymer Coverslip Bottom that has the highest optical quality (comparable to glass) and is ideally suitable for high-resolution microscopy. It is also available as a sticky-Slide 8 Well high without any bottom and the µ-Slide 8 Well high Glass Bottom for special microscopic applications.
The ibiTreat Surface
ibiTreat (tissue culture-treated) is our most recommended surface modification, because almost all adherent cells grow well on it without the need for any additional coating.
The ibidi µ-Slide 8 Well high allows for standard immunofluorescence protocols to be employed without the use of coverslips in an all-in-one chamber. All steps (e.g., cell cultivation, fixation, staining, and imaging) are carried out in the open well geometry. After staining, the sample can be observed through the coverslip bottom using high-resolution microscopy.
Fluorescence microscopy of HuH7 cells after mRNA transfection with eGFP (green). Actin cytoskeleton (Phalloidin, yellow), nuclei (DAPI, blue), mRNA (FISH,Quasar 670, red), 40x objective lens, oil immersion.
The µ-Slide 8 Well high enables high-resolution live cell imaging using different microscopy techniques.
Together with the ibidi Stage Top Incubation System, you can keep your cells happy for a long time by precisely controlling temperature, humidity, and CO2 concentration on your microscope.
Time-lapse phase contrast microscopy of rat fibroblast cells using the µ-Slide 8 Well high ibiTreat in combination with the ibidi Heating System and the ibidi Gas Incubation System. 63x objective lens, oil immersion, 3 min image intervals for 23 hours.