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Product outline:

  • A chambered coverslip with 8 individual wells and high walls for cell culture, immunofluorescence, and high-end microscopy
  • All-in-one 8 well chamber slide for cost-effective experiments with small cell numbers and low reagent volumes
  • High-resolution imaging through No. 1.5 polymer coverslip bottom with the highest optical quality
  • Suitable for various microscopy techniques (e.g., DIC, widefield fluorescence, confocal microscopy, two-photon microscopy, FRAP, FRET, FLIM, and LSFM)
  • Extra high individual walls to keep cross contamination between wells as low as possible

Applications

  • Cultivation and high-resolution microscopy of cells
  • Immunofluorescence staining and fluorescence microscopy of living and fixed cells
  • Live cell imaging over extended time periods
  • Transfection assays
  • Differential interference contrast (DIC) microscopy when used with a DIC lid

Technical specifications

Outer dimensions (w x l) 25.5 x 75.5 mm²
Number of wells 8
Dimensions of wells (w x l x h) 9.4 x 10.7 x 9.3 mm³
Volume per well 300 µl
Total height with/without lid 10.8/9.5 mm
Growth area per well 1.0 cm²
Coating area per well 2.2 cm²
Bottom: ibidi Polymer Coverslip

Technical features

  • Chambered coverslip with 8 independent wells and a non-removable polymer coverslip-bottom
  • ibiTreat (tissue culture-treated) surface for optimal cell adhesion
  • Imaging chamber slide with excellent optical quality for high-end microscopy
  • Individual well walls for minimizing well-to-well crosstalk and contaminations
  • Compatible with staining and fixation solutions
  • Biocompatible polymer material—no glue, no leaking
  • Also available as an adhesive version without a bottom: sticky-Slide 8 Well high
  • Also available with a Glass Coverslip Bottom: µ-Slide 8 Well high Glass Bottom for special microscopic applications
  • Additional version available with a 500 µm grid: µ-Slide 8 Well high Grid-500

The Principle of the µ-Slide 8 Well high

  • The Coverslip Bottom

    The µ-Slide 8 Well high comes with a thin ibidi Polymer Coverslip Bottom that has the highest optical quality (comparable to glass) and is ideally suitable for high-resolution microscopy. It is also available as a sticky-Slide 8 Well high without any bottom and the µ-Slide 8 Well high Glass Bottom for special microscopic applications.

  • The ibiTreat Surface

    ibiTreat (tissue culture-treated) is our most recommended surface modification, because almost all adherent cells grow well on it without the need for any additional coating.

Application examples

Immunofluorescence

The ibidi µ-Slide 8 Well high allows for standard immunofluorescence protocols to be employed without the use of coverslips in an all-in-one chamber. All steps (e.g., cell cultivation, fixation, staining, and imaging) are carried out in the open well geometry. After staining, the sample can be observed through the coverslip bottom using high-resolution microscopy.

Fluorescence microscopy of HuH7 cells after mRNA transfection with eGFP (green). Actin cytoskeleton (Phalloidin, yellow), nuclei (DAPI, blue), mRNA (FISH,Quasar 670, red), 40x objective lens, oil immersion.

Live cell imaging

The µ-Slide 8 Well high enables high-resolution live cell imaging using different microscopy techniques. 

Together with the ibidi Stage Top Incubation System, you can keep your cells happy for a long time by precisely controlling temperature, humidity, and CO2 concentration on your microscope.

Time-lapse phase contrast microscopy of rat fibroblast cells using the µ-Slide 8 Well high ibiTreat in combination with the ibidi Heating System and the ibidi Gas Incubation System. 63x objective lens, oil immersion, 3 min image intervals for 23 hours.

Ibidiibidi is a leading supplier for functional cell-based assays and advanced products for cellular microscopy. ibidi is located in Gräfelfing, Germany, close to Munich, and the US headquarters, ibidi USA Inc., is located in Fitchburg, Wisconsin.
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