Virion\Serion Immunomat™ SERION ELISA Analyzer Fully automated SERION ELISA analyzer for medium to high throughput applications
Infectious diseases immunoassays are tests for the presence of an antibody or an antigen and are used in the clinical setting as immunodiagnostics. The protein expressed in the sera of a blood sample can be detected using immunoassay techniques such as ELISA. Immunodiagnostics has found common use in the medical laboratory setting as a tool to quickly and accurately test for infectious diseases inpatient samples. These tests are routinely found in labs but also contribute greatly to the management of epidemics such as hepatitis, Zika disease, HIV-AIDS, and other sexually transmitted diseases, and in pediatrics.
The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is particularly suited to the determination of antibodies and antigens in the field of infectious serology. The reaction is based on the specific interaction of antibodies with their corresponding antigens. The test strips of the SERION ELISA antigen microtiter plate are coated with specific antibodies directed against the pathogen of interest. If antigens in the patient sample are present, they bind to the fixed antibody. A secondary antibody, which has been conjugated with the enzyme peroxidase, detects and binds to the immune complex.
The colourless substrate hydrogen peroxide (H2O2) and the chromogen tetra-methylbenzidine (TMB) are converted into a blue coloured product. Addition of stopping solution changes the colour to yellow. The signal intensity of this reaction product is proportional to the concentration of the antigen in the sample and is measured photometrically.
Contact us to discuss your analytical needs