ELISA & Immunoassays
Enzyme-linked immunoabsorbent assays (ELISAs) is one of the most popular applications of the microplate reader. An ELISA is used to measure the specific amount of an antigen, antibody, or other proteins. It is typically performed with colorimetric detection, but can be performed as well with luminescence, chemiluminescence, and fluorescence as well. As ELISAs are well characterized, easy to perform, and have many commercially available and bespoke variants, this technique is prevalent in most life science laboratories.