Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. Capturing multiple two-dimensional images at different depths in a sample enables the reconstruction of three-dimensional structures within an object. Confocal microscopes are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented precision in three-dimensional imaging and exact examination of subcellular structures and dynamic processes.
Confocal microscopes support the imaging needs of the life science research community with advanced innovation and technical expertise for the visualization, measurement and analysis of subcellular structures and biomolecules
Imaging live cells and specimens requires a “gentle” imaging technique which preserves physiological conditions and specimen integrity. Confocal microscopes integrate high-performance detectors and cameras, specialized illumination systems, imaging software and other dedicated components to make a live-cell imaging solution that meets the needs of scientists.
Cancer is a complex and heterogeneous disease caused by cells deficient in growth regulation, disrupting normal function and resulting in uncontrolled cell growth and proliferation. Confocal microscopes help researchers reveal insights into the mechanisms of cancer at the subcellular and molecular levels.
In order to better understand the nervous system, neuroscientists often investigate complex specimens using confocal microscopy to overcome challenges.
Fluorescence lifetime imaging provides additional information that can help you improve the quality of your confocal imaging. It can be particularly useful to discriminate fluorescence probes that have overlapping fluorescent emission spectra or to eliminate unwanted background fluorescent signals.
FLIM is a crucial application for measuring and quantifying imaging data. As lifetime information is independent of fluorophore concentration, it is extremely well suited for functional imaging. Functional imaging goes beyond the traditional recording of the location and concentration of molecular species and enables further investigation of molecular function, interactions, and their environment. super-resolution in three dimensions. Underlining the impact of super-resolution.
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