Ask an Expert: Quantifying rAAV and Other Viral Vector Quality Attributes with Multi-angle DLS and Nanoparticle Tracking Analysis Listen with ReadSpeaker Webinar

Quick, label-free methods with minimal sample prep for simultaneously measuring rAAV aggregation, capsid charge, and particle concentration.

Date recorded: July 15, 2021

Viral vectors, such as Recombinant Adeno-Associated Viruses (rAAV) Adenoviruses (AV) are increasingly being used in the development of gene therapies and vaccines. The efficient development of viral vectors order to develop effective therapies and vaccines presents a number of challenges that we will address in this webinar.

Viral vectors are typically produced in engineered cell lines in large bioreactors and then isolated from the bulk harvest through cell-lysis and multiple rounds of purification for example, via density gradient centrifugation, ion-exchange chromatography, and tangential flow filtration. Multiple assays are performed during this process to determine key analytical attributes for the determination of yield, efficacy, and safety, as well as to enable process understanding and control. For AAV vectors, these parameters include capsid/particle size and count, often measured using assays such as Elisa, AUC and TEM.

This webinar discusses the use of Multi-angle Dynamic Light Scattering (MADLS) and Nanoparticle Tracking Analysis as  label-free 5-minute methods to assess capsid yield and aggregation across the various viral vector processing steps. Real world examples will be elaborated on, including the use of MADLS to reduce the development time of rAAV-vectored gene therapies. Bringing effective, stable vaccines and therapeutics to market in a rapid and cost-effective manner has never been more important, and we look forward to using this time to describe how Malvern Panalytical delivers the information essential to this process in a simple and efficient manner.