Robust Higher-Order Multiplexing in Digital PCR by Color-Combination Application note

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There is a growing need in molecular biology to interrogate samples for higher number of biomarkers, beyond the 2 to 5 biomarkers typically addressable with standard PCR technologies. Here, we demonstrate a novel approach to increase the level of multiplexing in digital PCR up to 15-plex by detecting each target with 2 distinct fluorophores with a 6-color digital PCR system, a method called digital PCR by color combination.

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